Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KW3_RS0111295 Genome accession   NZ_AKGH01000001
Coordinates   2482756..2483346 (-) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2477756..2488346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW3_RS0111275 aroB 2478683..2479768 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  KW3_RS0111280 aroK 2479796..2480320 (-) 525 WP_000818616.1 shikimate kinase AroK -
  KW3_RS0111285 pilQ 2480507..2482222 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  KW3_RS0111290 pilP 2482248..2482766 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  KW3_RS0111295 pilO 2482756..2483346 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  KW3_RS0111300 pilN 2483339..2483923 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  KW3_RS0111305 pilM 2483913..2484929 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  KW3_RS0111310 - 2485072..2487549 (+) 2478 WP_000668733.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=64862 KW3_RS0111295 WP_000157690.1 2482756..2483346(-) (pilO) [Vibrio cholerae H1]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=64862 KW3_RS0111295 WP_000157690.1 2482756..2483346(-) (pilO) [Vibrio cholerae H1]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGTGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694

Multiple sequence alignment