Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   KW3_RS0111300 Genome accession   NZ_AKGH01000001
Coordinates   2483339..2483923 (-) Length   194 a.a.
NCBI ID   WP_000902745.1    Uniprot ID   A0A0H6BVQ2
Organism   Vibrio cholerae H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2478339..2488923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW3_RS0111275 aroB 2478683..2479768 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  KW3_RS0111280 aroK 2479796..2480320 (-) 525 WP_000818616.1 shikimate kinase AroK -
  KW3_RS0111285 pilQ 2480507..2482222 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  KW3_RS0111290 pilP 2482248..2482766 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  KW3_RS0111295 pilO 2482756..2483346 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  KW3_RS0111300 pilN 2483339..2483923 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  KW3_RS0111305 pilM 2483913..2484929 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  KW3_RS0111310 - 2485072..2487549 (+) 2478 WP_000668733.1 penicillin-binding protein 1A -
  KW3_RS0111315 oxyR 2487623..2488516 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 21964.42 Da        Isoelectric Point: 9.9503

>NTDB_id=64863 KW3_RS0111300 WP_000902745.1 2483339..2483923(-) (pilN) [Vibrio cholerae H1]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQAGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=64863 KW3_RS0111300 WP_000902745.1 2483339..2483923(-) (pilN) [Vibrio cholerae H1]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCAGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAACAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGCGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H6BVQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

100

100

1
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552

Multiple sequence alignment