Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1O00_RS18625 Genome accession   NZ_CP091140
Coordinates   4114272..4115381 (-) Length   369 a.a.
NCBI ID   WP_278174304.1    Uniprot ID   -
Organism   Aeromonas veronii strain b1-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4109272..4120381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1O00_RS18595 (L1O00_18570) - 4109736..4110743 (-) 1008 WP_278174302.1 Fe(3+) ABC transporter substrate-binding protein -
  L1O00_RS18600 (L1O00_18575) glnK 4111002..4111340 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  L1O00_RS18605 (L1O00_18580) arfB 4111542..4111958 (+) 417 WP_278169279.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  L1O00_RS18610 (L1O00_18585) - 4111990..4112481 (+) 492 WP_278169281.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  L1O00_RS18615 (L1O00_18590) - 4112831..4113256 (-) 426 WP_005340360.1 hypothetical protein -
  L1O00_RS18620 (L1O00_18595) yaaA 4113451..4114224 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  L1O00_RS18625 (L1O00_18600) pilU 4114272..4115381 (-) 1110 WP_278174304.1 type IVa pilus ATPase TapU Machinery gene
  L1O00_RS18630 (L1O00_18605) pilT 4115410..4116444 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  L1O00_RS18635 (L1O00_18610) - 4116484..4117185 (+) 702 WP_278169286.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1O00_RS18640 (L1O00_18615) proC 4117327..4118151 (+) 825 WP_278169288.1 pyrroline-5-carboxylate reductase -
  L1O00_RS18645 (L1O00_18620) - 4118163..4118714 (+) 552 WP_047436998.1 YggT family protein -
  L1O00_RS18650 (L1O00_18625) yggU 4118714..4119016 (+) 303 WP_076495786.1 DUF167 family protein YggU -
  L1O00_RS18655 (L1O00_18630) - 4119060..4119479 (+) 420 WP_278169293.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41090.14 Da        Isoelectric Point: 6.5045

>NTDB_id=647983 L1O00_RS18625 WP_278174304.1 4114272..4115381(-) (pilU) [Aeromonas veronii strain b1-3]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=647983 L1O00_RS18625 WP_278174304.1 4114272..4115381(-) (pilU) [Aeromonas veronii strain b1-3]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGTGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGTTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATTTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATTTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTTGGGATCGACACCGAGTCGTTCGATGT
AGCGCTAAAAAGCTCGCTGCGTCAGGCGCCAGACGTGATCCTGATCGGCGAAATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGTATC
CTCCATCTGGTGCCGCAAGAGAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTTAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAGTCCACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACAGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36