Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   L1A13_RS00220 Genome accession   NZ_CP090889
Coordinates   36313..37254 (+) Length   313 a.a.
NCBI ID   WP_000249552.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain CH2439     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 31313..42254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1A13_RS00200 (L1A13_00200) - 31379..33196 (+) 1818 WP_001220922.1 acyltransferase family protein -
  L1A13_RS00205 (L1A13_00205) nagA 33349..34500 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  L1A13_RS00210 (L1A13_00210) - 34663..35721 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  L1A13_RS00215 (L1A13_00215) - 35872..36237 (+) 366 WP_000286415.1 DUF1033 family protein -
  L1A13_RS00220 (L1A13_00220) comGA/cglA/cilD 36313..37254 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  L1A13_RS00225 (L1A13_00225) comGB/cglB 37202..38218 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  L1A13_RS00230 (L1A13_00230) comGC/cglC 38220..38546 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  L1A13_RS00235 (L1A13_00235) comGD/cglD 38539..38943 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  L1A13_RS00240 (L1A13_00240) comGE/cglE 38906..39208 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  L1A13_RS00245 (L1A13_00245) comGF/cglF 39171..39632 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  L1A13_RS00250 (L1A13_00250) comGG/cglG 39610..40023 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  L1A13_RS00255 (L1A13_00255) - 40160..40754 (+) 595 Protein_35 methyltransferase domain-containing protein -
  L1A13_RS00260 (L1A13_00260) comYH 40815..41768 (+) 954 WP_000345099.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35587.57 Da        Isoelectric Point: 6.0083

>NTDB_id=646100 L1A13_RS00220 WP_000249552.1 36313..37254(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain CH2439]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=646100 L1A13_RS00220 WP_000249552.1 36313..37254(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain CH2439]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGTCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACATCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364