Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LY388_RS07805 Genome accession   NZ_CP090387
Coordinates   484451..484882 (-) Length   143 a.a.
NCBI ID   WP_057563244.1    Uniprot ID   -
Organism   Vibrio cholerae strain GXFL1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479451..489882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY388_RS07785 (LY388_07785) coaE 479944..480552 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  LY388_RS07790 (LY388_07790) pilD 480549..481424 (-) 876 WP_000418748.1 A24 family peptidase Machinery gene
  LY388_RS07795 (LY388_07795) pilC 481482..482708 (-) 1227 WP_148504744.1 type II secretion system F family protein Machinery gene
  LY388_RS07800 (LY388_07800) pilB 482761..484449 (-) 1689 WP_057563243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LY388_RS07805 (LY388_07805) pilA 484451..484882 (-) 432 WP_057563244.1 pilin Machinery gene
  LY388_RS07810 (LY388_07810) nadC 485127..486017 (-) 891 WP_000665277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LY388_RS07815 (LY388_07815) ampD 486319..486864 (+) 546 WP_000567314.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LY388_RS07820 (LY388_07820) fldB 487013..487531 (-) 519 WP_000690112.1 flavodoxin FldB -
  LY388_RS07825 (LY388_07825) xerD 487669..488577 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  LY388_RS07830 (LY388_07830) dsbC 488594..489346 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14653.68 Da        Isoelectric Point: 9.4343

>NTDB_id=641920 LY388_RS07805 WP_057563244.1 484451..484882(-) (pilA) [Vibrio cholerae strain GXFL1-4]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLENGSFPTSSAVPVPSTS
TGAITYSSGASGAGSIIFTFAASGASPSVVTKNVTLARDGDGKWICTSSIGSDFRPKGCAASQ

Nucleotide


Download         Length: 432 bp        

>NTDB_id=641920 LY388_RS07805 WP_057563244.1 484451..484882(-) (pilA) [Vibrio cholerae strain GXFL1-4]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCGTGTT
GGCTGCCATTGCAGTACCTCAGTACCAAAAATACGTTGCGAAAAGTGAAGCAGCAGCAGCACTGGCTTCAATCACCGGGC
ATAGAACAAACGTTGAAACATACGTATTGGAAAATGGTTCGTTCCCAACATCATCTGCCGTGCCTGTTCCATCAACAAGT
ACAGGAGCAATTACTTACAGCTCAGGGGCATCTGGTGCAGGCAGCATCATCTTCACATTTGCAGCCAGTGGTGCAAGCCC
TAGCGTTGTGACTAAAAACGTCACTTTAGCTCGAGACGGAGATGGCAAATGGATCTGTACATCTTCTATTGGCTCAGATT
TCAGACCCAAAGGCTGTGCGGCCTCCCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.658

100

0.538
  pilA Vibrio cholerae strain A1552

50.658

100

0.538
  pilA Vibrio cholerae C6706

50.658

100

0.538
  pilA Pseudomonas aeruginosa PAK

45.946

100

0.476
  pilA Acinetobacter baumannii strain A118

47.482

97.203

0.462
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.254

99.301

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.845

99.301

0.406
  pilA Vibrio parahaemolyticus RIMD 2210633

44.615

90.909

0.406
  comP Acinetobacter baylyi ADP1

37.762

100

0.378