Detailed information NTG0641656

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LXN51_RS24435
• Genome accession: NZ_CP090355
• Coordinates: 5315195..5316304 (-)
• Length: 369 a.a.
• NCBI ID: WP_085701916.1
• Uniprot ID: -
• Organism: Pseudomonas kribbensis strain H38E-23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5310195..5321304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXN51_RS24420 (LXN51_24420)	-	5310997..5312388 (+)	1392	WP_085701918.1	GntP family permease	-
LXN51_RS24425 (LXN51_24425)	hbdH	5312396..5313169 (+)	774	WP_007952053.1	3-hydroxybutyrate dehydrogenase	-
LXN51_RS24430 (LXN51_24430)	-	5313233..5315188 (+)	1956	WP_085701917.1	acetoacetate--CoA ligase	-
LXN51_RS24435 (LXN51_24435)	pilU	5315195..5316304 (-)	1110	WP_085701916.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LXN51_RS24440 (LXN51_24440)	-	5316432..5318969 (+)	2538	WP_085701915.1	PAS domain-containing protein	-
LXN51_RS24445 (LXN51_24445)	-	5319020..5319295 (+)	276	WP_085695956.1	peptidylprolyl isomerase	-
LXN51_RS24450 (LXN51_24450)	-	5319409..5320341 (-)	933	WP_085701914.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41064.95 Da
• Isoelectric Point: 6.8358

>NTDB_id=641656 LXN51_RS24435 WP_085701916.1 5315195..5316304(-) (pilU) [Pseudomonas kribbensis strain H38E-23]
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLSEQPFKPGEVATVAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPSVLLESVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHALGNHLKAFVSQRLVRTRDGQRRAAAEVMLGTPTIADLIRRNDFGELKTIMEKSAELGMQTFDGAL
FALVAEGVIDEDEALKNADSVNNLRLRLKLHADTMSSPPPSTGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=641656 LXN51_RS24435 WP_085701916.1 5315195..5316304(-) (pilU) [Pseudomonas kribbensis strain H38E-23]
ATGGAAATCGATGCATTGCTCAAACAACTGGCGAGCCGCCACGGTTCGGATCTGTTCCTGTCCACGGGCGCACCGCCCAG
TGCGCGGTTCGACGGGGTGTTGACGCCGTTGAGCGAGCAGCCCTTCAAACCCGGTGAGGTGGCCACTGTCGCCGCTTCCC
TGATGGACGCTGAACAACGTCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCCCGCACCGGCATCGGTCGC
TTTCGGGTGAATATCTTCAAGCAACGCAACGACGTGTCAATCGTGATCCGTAACGTCAAACTCGATATTCCGCGATTCGA
AGACTTGAAACTGCCGTCGGTATTGCTCGAATCGGTGATGCTCAAGCAAGGGCTGATCCTGTTCGTCGGCGCCACCGACT
CGGGTAAATCCACGTCGCTGGCGGCGCTGATCGATCATCGCAATCGCCACAGTAGCGGTCACATCATCACCATTGAAGAC
CCGGTGGAATACATCCATCGCCATAAGAAGTCGATCATCAACCAGCGCGAAGTCGGAGTCGACACGCGCAGCTTTCACGC
TGCGCTGAAAAACACCCTGCGCCAGGCCCCCGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAGACCATGGAGCATG
CGCTGGCTTTCGCCGAAACCGGGCACCTTGTGTTGTCGACATTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCCGAAGAACGCCGGCCACAATTGCTGCATGCCTTGGGCAATCATCTCAAGGCGTTCGTTTCCCAGCG
TCTGGTCCGAACCCGGGACGGTCAGCGCCGGGCCGCTGCCGAGGTGATGCTCGGTACGCCGACGATTGCTGACCTGATCC
GGCGCAATGACTTTGGCGAGCTGAAAACCATCATGGAGAAATCGGCCGAACTGGGCATGCAGACCTTTGACGGTGCACTG
TTTGCGCTGGTGGCAGAAGGGGTGATCGATGAAGACGAGGCCCTGAAAAACGCAGATTCGGTCAACAACCTGCGCCTGCG
TCTGAAGCTCCACGCCGACACCATGTCGTCCCCACCGCCATCGACTGGCGAGTGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	96.206	0.542
pilU	Acinetobacter baylyi ADP1	53.693	95.393	0.512
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.823	91.87	0.366
pilT	Pseudomonas aeruginosa PAK	39.244	93.225	0.366
pilT	Legionella pneumophila strain Lp02	39.82	90.515	0.36
pilT	Legionella pneumophila strain ERS1305867	39.82	90.515	0.36