Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I533_RS14375 Genome accession   NC_023045
Coordinates   3270606..3271718 (-) Length   370 a.a.
NCBI ID   WP_012519492.1    Uniprot ID   F2GD64
Organism   Alteromonas mediterranea MED64     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3265606..3276718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I533_RS14350 (I533_14380) - 3267163..3267891 (+) 729 WP_012519487.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I533_RS14355 (I533_14385) gshB 3268111..3269061 (+) 951 WP_024016795.1 glutathione synthase -
  I533_RS14360 (I533_14390) - 3269173..3269739 (+) 567 WP_043960686.1 YqgE/AlgH family protein -
  I533_RS14365 (I533_14395) ruvX 3269732..3270163 (+) 432 WP_012519490.1 Holliday junction resolvase RuvX -
  I533_RS14370 (I533_14400) - 3270254..3270580 (-) 327 WP_015067844.1 DUF2007 domain-containing protein -
  I533_RS14375 (I533_14405) pilU 3270606..3271718 (-) 1113 WP_012519492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I533_RS14380 (I533_14410) pilT 3271727..3272770 (-) 1044 WP_012519493.1 type IV pilus twitching motility protein PilT Machinery gene
  I533_RS14385 (I533_14415) - 3272798..3273496 (+) 699 WP_012519494.1 YggS family pyridoxal phosphate-dependent enzyme -
  I533_RS14390 (I533_14420) proC 3273548..3274369 (+) 822 WP_012519495.1 pyrroline-5-carboxylate reductase -
  I533_RS14395 (I533_14425) - 3274390..3274926 (+) 537 WP_012519496.1 YggT family protein -
  I533_RS14400 (I533_14430) - 3275011..3275451 (+) 441 WP_015067845.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40798.64 Da        Isoelectric Point: 6.0479

>NTDB_id=64079 I533_RS14375 WP_012519492.1 3270606..3271718(-) (pilU) [Alteromonas mediterranea MED64]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTDSATSEEASLALVHEAMTDKQRDEFHTSKECNFAIVRDGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGRIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=64079 I533_RS14375 WP_012519492.1 3270606..3271718(-) (pilU) [Alteromonas mediterranea MED64]
ATGCTAGATTCACTACTTCAAAAAATGGTTGATACCAACGCGTCAGATTTATTTGTCACTGCGGGCTTTCCGGTTAGCGC
TAAAATAAACGGTAAACTTACCCCTGTCACTGATAGCGCAACGTCAGAAGAGGCATCACTCGCGCTGGTTCATGAAGCCA
TGACCGATAAGCAGCGTGACGAGTTTCATACGTCGAAGGAATGCAACTTTGCTATCGTGCGTGATGGCATTGGTCGTTTT
CGTTGTTCTGCATTTTGGCAAAGAGACCAAGCGGGTATGGTTATCCGTCGTATTGTGACGCAAATACCGAAAGCCGATGA
CTTGGGGCTGCCCCGAGTATTGAAAGAAATTATCATGTCTAAGCGCGGTCTTGTTCTATTTGTAGGTGGGACAGGTACGG
GTAAATCAACCTCGCTAGCTGCACTTATTGGGCATAGGAATGAAAACTCTCATGGGCATATACTGACCATTGAGGACCCT
ATTGAATTTGTGCACGAGCATAAAAACTGTGTGATTACACAGCGTGAGGTGGGTATTGATACCCAGTCATTCGACGATGC
GCTTAAGAGCTCGTTGCGACAAGCGCCAGACGTTATTCTAATTGGTGAGATTCGGTCTATGGAAACCATGGAATATGCCA
TGTCGTTCGCCGATACGGGCCACTTGTGTGTGGCAACGCTGCACGCGAACAATGCGAACCAAGCCATAGAGCGTATTATG
CACTTAGCGCCAAAAGATCAGCACGACAAGCTGCGCTTTGACTTAAGCTTGAACATTCGTGCTATTGTTGCCCAGCAACT
GGTACCGACGCCAGACGGTAAAGGGCGTGTGGCTGCCATCGAGATACTGCTTAATTCACCATTGGTGCGTGACCTTATTC
AGCGCAATGAAATAGGCGCGTTGAAAGAAGCCATGAAAAAAGGCAAAGAGCAGGGCATGCAAAGTTTTGATATGGCGCTT
TACGACCTTTACAAGGCCGGCCGAATTGACATGGATCAGGCGCTGCATCATGCTGACTCACCGAATGATTTACGCTTAAT
GATTAAGCTGGATACCAGCGATGGCTCAAGCCTAGGTACGCTGTCTAATGTGTCGATTGATATGGACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2GD64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

57.26

98.649

0.565
  pilU Vibrio cholerae strain A1552

55.435

99.459

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

91.892

0.384
  pilT Acinetobacter nosocomialis M2

39.65

92.703

0.368
  pilT Acinetobacter baumannii D1279779

39.65

92.703

0.368
  pilT Acinetobacter baumannii strain A118

39.65

92.703

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.067

92.703

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.729

88.919

0.362
  pilT Vibrio cholerae strain A1552

40.729

88.919

0.362

Multiple sequence alignment