Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LT988_RS13610 Genome accession   NZ_CP089309
Coordinates   3003165..3004322 (+) Length   385 a.a.
NCBI ID   WP_232406110.1    Uniprot ID   -
Organism   Thiocapsa bogorovii strain BBS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998165..3009322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT988_RS13585 (LT988_13585) - 2998587..2999996 (-) 1410 WP_232406105.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
  LT988_RS13590 (LT988_13590) - 3000181..3000618 (-) 438 WP_232406106.1 type II toxin-antitoxin system VapC family toxin -
  LT988_RS13595 (LT988_13595) - 3000605..3000865 (-) 261 WP_232406107.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  LT988_RS13600 (LT988_13600) - 3001024..3001800 (-) 777 WP_232406108.1 (Fe-S)-binding protein -
  LT988_RS13605 (LT988_13605) pilT 3001971..3003008 (+) 1038 WP_232406109.1 type IV pilus twitching motility protein PilT Machinery gene
  LT988_RS13610 (LT988_13610) pilU 3003165..3004322 (+) 1158 WP_232406110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LT988_RS13615 (LT988_13615) - 3004386..3005291 (+) 906 WP_232406111.1 TIGR00730 family Rossman fold protein -
  LT988_RS13620 (LT988_13620) - 3005305..3007575 (+) 2271 WP_232406112.1 TonB-dependent receptor -
  LT988_RS13625 (LT988_13625) - 3007600..3008352 (-) 753 WP_232406113.1 Fic family protein -
  LT988_RS13630 (LT988_13630) ppk2 3008371..3009219 (-) 849 WP_232406114.1 polyphosphate kinase 2 -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42994.27 Da        Isoelectric Point: 7.1153

>NTDB_id=636351 LT988_RS13610 WP_232406110.1 3003165..3004322(+) (pilU) [Thiocapsa bogorovii strain BBS]
MDLNRAIEQLLSMVVERKASDLFITAGWPPSVKIDGAIYPATKHPLSAEQAHDMVLGLMNDRQREEFQATKECQFAIDRP
HLGRFRVSAFVQREATGMVLRRIETDIPTIEALKLPSILRELALTKRGMVIFVGGTGTGKSTSLAALVGYRNRHSSGHII
TVEDPIEFVHEHHGCIITQREVGVDTESYEVALKNTLRQAPDVILIGEIRTRATMEYAIAFAETGHLVLATLHANNANQA
LDRIISFFPDDSRNQLLLDLSLNLKAVVAQQLAPRKSGQGRRAVVEILLNTPLASDLIRKGEVHKLKELMKKSNEQGMIT
FDQALFNLYREGEISYEDALRYADSANEVRLVIKLQGGAAQREATDRMIDGVSLVHDNEYRGRRP

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=636351 LT988_RS13610 WP_232406110.1 3003165..3004322(+) (pilU) [Thiocapsa bogorovii strain BBS]
ATGGACCTGAATCGTGCAATCGAGCAACTGCTGAGCATGGTGGTCGAGCGCAAGGCGTCCGACCTCTTCATCACCGCGGG
CTGGCCGCCCAGCGTCAAGATCGACGGGGCTATCTATCCCGCGACAAAGCATCCCTTGTCGGCCGAGCAAGCGCACGACA
TGGTGCTCGGTCTCATGAACGATCGCCAGCGCGAGGAGTTTCAGGCGACCAAGGAATGTCAGTTCGCGATCGATCGGCCT
CATCTCGGTCGCTTTCGCGTCAGTGCATTCGTCCAGCGCGAGGCAACCGGGATGGTCCTGCGCCGGATCGAGACCGACAT
TCCGACGATCGAGGCACTCAAGCTCCCCTCGATCCTGCGCGAGCTGGCATTGACCAAGCGCGGCATGGTGATCTTTGTCG
GCGGCACCGGGACGGGTAAATCGACCTCGCTCGCCGCGCTGGTGGGATATCGCAACCGGCACAGCTCGGGTCACATCATC
ACGGTCGAGGATCCGATCGAGTTCGTCCACGAGCATCACGGCTGCATCATCACCCAGCGCGAGGTGGGCGTGGATACCGA
GTCCTACGAGGTCGCGCTCAAGAACACACTGCGTCAGGCCCCGGATGTCATCCTGATCGGCGAGATCCGCACCCGCGCGA
CGATGGAATACGCCATCGCCTTCGCCGAGACCGGCCATCTCGTTTTGGCGACCCTGCACGCCAACAACGCCAACCAAGCA
CTGGACCGCATCATCAGCTTCTTTCCGGACGACTCGCGCAACCAGCTTCTGCTCGATCTCTCGCTGAATCTCAAGGCCGT
CGTGGCGCAACAGCTCGCCCCGCGCAAGAGCGGTCAGGGCCGTCGGGCCGTCGTAGAGATCCTGTTGAACACGCCGTTGG
CCTCCGATCTCATCCGCAAGGGCGAGGTGCACAAACTCAAAGAGCTGATGAAGAAGTCCAACGAGCAGGGCATGATCACC
TTCGATCAGGCCCTCTTCAATCTCTACCGAGAGGGCGAGATCAGCTACGAGGATGCGCTGCGTTATGCAGACTCGGCCAA
CGAGGTGCGGCTCGTCATCAAGCTCCAAGGCGGTGCCGCGCAGCGCGAGGCGACCGATCGTATGATCGACGGGGTTTCTC
TCGTGCACGACAACGAGTACCGCGGGCGGCGACCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.048

97.922

0.647
  pilU Acinetobacter baylyi ADP1

62.921

92.468

0.582
  pilU Vibrio cholerae strain A1552

58.939

92.987

0.548
  pilT Acinetobacter baylyi ADP1

41.399

89.091

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

87.532

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

86.234

0.366
  pilT Vibrio cholerae strain A1552

42.47

86.234

0.366
  pilT Legionella pneumophila strain Lp02

41.742

86.494

0.361
  pilT Legionella pneumophila strain ERS1305867

41.742

86.494

0.361