Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IUZ65_RS13785 Genome accession   NZ_CP087590
Coordinates   3027892..3029139 (+) Length   415 a.a.
NCBI ID   WP_195704264.1    Uniprot ID   -
Organism   Vibrio sp. VB16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3022892..3034139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUZ65_RS13760 (IUZ65_013760) pdhR 3024379..3025146 (-) 768 WP_195704260.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IUZ65_RS13765 (IUZ65_013765) ampD 3025430..3025981 (-) 552 WP_195704261.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IUZ65_RS13770 (IUZ65_013770) nadC 3026168..3027055 (+) 888 WP_195704262.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IUZ65_RS23415 - 3027332..3027835 (+) 504 WP_195704263.1 pilin -
  IUZ65_RS13785 (IUZ65_013785) pilB 3027892..3029139 (+) 1248 WP_195704264.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  IUZ65_RS13790 (IUZ65_013790) - 3029176..3030399 (+) 1224 WP_195704265.1 type II secretion system F family protein -
  IUZ65_RS13795 (IUZ65_013795) pilD 3030436..3031305 (+) 870 WP_195704266.1 A24 family peptidase Machinery gene
  IUZ65_RS13800 (IUZ65_013800) coaE 3031307..3031909 (+) 603 WP_195704267.1 dephospho-CoA kinase -
  IUZ65_RS13805 (IUZ65_013805) zapD 3031948..3032688 (+) 741 WP_195704268.1 cell division protein ZapD -
  IUZ65_RS13810 (IUZ65_013810) yacG 3032713..3032910 (+) 198 WP_195705124.1 DNA gyrase inhibitor YacG -
  IUZ65_RS13815 (IUZ65_013815) rplS 3033029..3033382 (-) 354 WP_195704269.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 46094.09 Da        Isoelectric Point: 6.7467

>NTDB_id=630233 IUZ65_RS13785 WP_195704264.1 3027892..3029139(+) (pilB) [Vibrio sp. VB16]
MDIIRNISNDQLSELVPLSDEELNITSAELQRNDPISRYINQVLLEAIQKRSSDIHFEPYEHKYRIRLRCDGVLVEIDSP
PSQLANRLSSRIKVMANLDIAEKRLPQDGRIKHTLTSKQSVDIRVSTLPTLWGEKIVLRLLKTQSVPLDITALGLSQTQL
DLYVSAISKPQGLILITGPTGSGKTISLYTALTYLNTPEKNISTAEDPIEINLLGINQVQMNDKIDLNFTSTLRALLRQD
PDIIMVGEIRDIESAEIVIKAAQTGHLVLSTLHTNSASEAVTRLLNMGVDKFSLASCLSLVVAQRLTRALCPVCKEFDLE
TSSNRKLNPRGSLQIYKANPHGCSDCLNGYSGLIGIFELLNIDKTLMRTIERQNSASKINDIALENGFIPLSESGKNKLI
EGITSIKELQRTLVF

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=630233 IUZ65_RS13785 WP_195704264.1 3027892..3029139(+) (pilB) [Vibrio sp. VB16]
ATGGATATAATTAGAAACATCAGCAATGACCAACTATCTGAATTGGTGCCTCTTTCTGATGAAGAACTAAACATAACTAG
TGCTGAGTTGCAAAGAAATGACCCTATTAGCCGTTATATCAATCAGGTTTTGTTAGAGGCAATACAGAAAAGATCCTCAG
ATATTCATTTCGAGCCTTATGAACATAAATACCGTATTCGCTTACGATGCGATGGTGTATTAGTCGAGATAGATTCTCCG
CCCTCCCAATTAGCAAATAGGCTGTCTTCTAGAATAAAAGTAATGGCCAACTTGGATATTGCAGAGAAAAGACTTCCGCA
AGATGGGCGGATTAAACACACGCTAACGTCTAAACAGAGCGTTGATATTCGCGTCTCTACATTGCCAACATTATGGGGAG
AAAAGATCGTCCTTAGATTGCTCAAAACACAATCAGTCCCATTAGACATAACAGCCCTAGGTTTATCCCAAACGCAATTG
GACCTCTATGTGTCTGCAATTAGTAAACCTCAAGGATTAATATTAATCACCGGCCCTACAGGAAGTGGTAAAACCATTTC
ACTATATACCGCGCTCACCTATCTCAATACACCAGAAAAGAACATATCAACGGCTGAAGATCCGATCGAGATTAACCTAT
TAGGTATCAATCAAGTACAAATGAACGACAAAATCGACCTGAACTTCACCTCCACCCTAAGAGCCCTTCTAAGACAAGAC
CCTGACATTATTATGGTCGGAGAAATTCGTGATATTGAGAGCGCTGAGATTGTAATTAAAGCGGCACAAACTGGTCACCT
TGTACTTTCCACACTGCATACCAACTCAGCTAGCGAAGCAGTTACTCGACTGCTTAACATGGGGGTAGATAAATTTAGCC
TAGCCTCATGCTTATCATTAGTCGTCGCGCAGAGACTTACACGGGCATTATGTCCTGTATGCAAAGAATTCGATTTGGAA
ACCTCTTCGAATCGAAAGCTAAACCCTCGAGGTTCTCTACAAATATACAAAGCTAATCCTCACGGATGCTCCGACTGTCT
CAACGGTTACTCAGGGTTGATTGGTATATTCGAATTGCTAAACATAGACAAAACCCTTATGAGAACAATTGAACGCCAAA
ACAGTGCTTCTAAAATAAACGACATAGCGTTAGAGAATGGTTTTATTCCTCTGTCTGAATCAGGGAAAAATAAATTAATT
GAAGGGATAACGAGTATCAAAGAATTGCAACGAACCTTAGTCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

57.108

100

0.571
  pilB Vibrio campbellii strain DS40M4

56.311

99.277

0.559
  pilB Vibrio parahaemolyticus RIMD 2210633

55.825

99.277

0.554
  pilB Legionella pneumophila strain ERS1305867

51.117

97.108

0.496
  pilB Acinetobacter baylyi ADP1

53.646

92.53

0.496
  pilB Acinetobacter baumannii D1279779

52.604

92.53

0.487
  pilF Neisseria gonorrhoeae MS11

49.361

94.217

0.465
  pilF Thermus thermophilus HB27

43.198

100

0.436
  pilB Haemophilus influenzae 86-028NP

46.134

93.494

0.431
  pilB Haemophilus influenzae Rd KW20

45.876

93.494

0.429
  pilB Glaesserella parasuis strain SC1401

44.703

93.253

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.194

92.048

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40.806

95.663

0.39
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.722

93.494

0.381