Detailed information NTG0628118

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY68_RS14175
• Genome accession: NZ_CP087205
• Coordinates: 3096783..3097892 (+)
• Length: 369 a.a.
• NCBI ID: WP_163909856.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-053
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3091783..3102892

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY68_RS14160 (LOY68_14140)	-	3092729..3093664 (+)	936	WP_265045330.1	sugar kinase	-
LOY68_RS14165 (LOY68_14145)	-	3093786..3094061 (-)	276	WP_265045332.1	peptidylprolyl isomerase	-
LOY68_RS14170 (LOY68_14150)	-	3094114..3096651 (-)	2538	WP_265045333.1	PAS domain-containing sensor histidine kinase	-
LOY68_RS14175 (LOY68_14155)	pilU	3096783..3097892 (+)	1110	WP_163909856.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY68_RS14180 (LOY68_14160)	-	3098002..3099957 (-)	1956	WP_265045335.1	acetoacetate--CoA ligase	-
LOY68_RS14185 (LOY68_14165)	hbdH	3100080..3100853 (-)	774	WP_265045337.1	3-hydroxybutyrate dehydrogenase	-
LOY68_RS14190 (LOY68_14170)	-	3100861..3102252 (-)	1392	WP_265045338.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41374.26 Da
• Isoelectric Point: 6.0972

>NTDB_id=628118 LOY68_RS14175 WP_163909856.1 3096783..3097892(+) (pilU) [Pseudomonas sp. B21-053]
MEIDLLLRQLASQEGSDLYLSTGAPPSARFDGVLKPLSEQRFKPGEIAAIAESIMDAEQRLEFDRELEMNLAISLAGVGR
FRINLFKQRNDVSIVARNIKLDIPRFEDLNLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVISTLHAHNANQALDRV
INFFPQERREQLLHDLANNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIADLIRRNEFDELKGIMEKSVELGMQTFDGAL
YALVVEGAISEDEALKHADSLNNLRLRLKLHADTSPSPQPPSGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=628118 LOY68_RS14175 WP_163909856.1 3096783..3097892(+) (pilU) [Pseudomonas sp. B21-053]
ATGGAAATCGATTTATTGTTGCGGCAACTGGCCAGCCAAGAGGGCTCCGATCTTTACCTCTCCACCGGCGCGCCACCCAG
TGCACGGTTCGATGGCGTGCTCAAACCCTTGTCCGAACAGCGGTTCAAACCGGGGGAAATCGCGGCCATCGCCGAGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGGGAACTGGAAATGAACTTGGCGATCTCCCTGGCCGGGGTCGGACGC
TTTCGGATCAACCTGTTCAAACAGCGCAATGACGTGTCGATCGTCGCGCGTAACATCAAGCTCGACATCCCGCGCTTCGA
AGACCTCAACCTGCCGCCGGTGCTGCTCGAAACCGTGATGCTCAAGCAAGGCCTGATGCTGTTTGTCGGCGCCACCGACT
CGGGCAAGTCCACGTCCCTGGCGGCGCTGATCGATTACCGCAACCGCCACAGCAGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAAAAGTCGATTATCAACCAGCGTGAGGTCGGCGTTGATACCCGCAGTTTTCATGC
AGCGTTGAAAAACACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAAATTCGCGACCGCGAGACCATGGAGCACG
CGCTGACCTTTGCTGATACCGGCCATTTGGTGATCTCCACCCTGCATGCGCACAACGCCAATCAGGCGCTGGACCGCGTC
ATCAACTTCTTCCCCCAGGAGCGCCGCGAACAACTGCTGCACGACCTGGCCAACAACCTCAAGGCGTTCGTATCCCAGCG
CCTGGTGCGCACCCGCGACGGCCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGGACGCCGACCATTGCCGATTTGATCC
GGCGCAATGAGTTTGATGAGCTTAAAGGGATTATGGAGAAGTCGGTGGAATTGGGGATGCAGACATTTGACGGGGCGTTG
TACGCGTTGGTCGTGGAGGGGGCGATCAGTGAGGATGAGGCGTTGAAGCATGCGGATTCGTTGAACAATCTGCGGTTACG
GTTGAAGTTGCATGCCGATACGTCGCCAAGCCCCCAACCACCTTCCGGCGAATGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	96.206	0.539
pilU	Acinetobacter baylyi ADP1	53.125	95.393	0.507
pilU	Vibrio cholerae strain A1552	52	94.851	0.493
pilT	Pseudomonas stutzeri DSM 10701	40.988	93.225	0.382
pilT	Pseudomonas aeruginosa PAK	40.407	93.225	0.377
pilT	Legionella pneumophila strain ERS1305867	40.351	92.683	0.374
pilT	Legionella pneumophila strain Lp02	40.351	92.683	0.374
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36