Detailed information NTG0627814

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY28_RS11350
• Genome accession: NZ_CP087194
• Coordinates: 2491772..2492881 (+)
• Length: 369 a.a.
• NCBI ID: WP_086946288.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2486772..2497881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY28_RS11335 (LOY28_11330)	-	2487766..2488701 (+)	936	WP_192351068.1	sugar kinase	-
LOY28_RS11340 (LOY28_11335)	-	2488773..2489048 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
LOY28_RS11345 (LOY28_11340)	-	2489115..2491652 (-)	2538	WP_258613848.1	PAS domain S-box protein	-
LOY28_RS11350 (LOY28_11345)	pilU	2491772..2492881 (+)	1110	WP_086946288.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY28_RS11355 (LOY28_11350)	-	2492942..2494897 (-)	1956	WP_258613849.1	acetoacetate--CoA ligase	-
LOY28_RS11360 (LOY28_11355)	hbdH	2495050..2495823 (-)	774	WP_018927665.1	3-hydroxybutyrate dehydrogenase	-
LOY28_RS11365 (LOY28_11360)	-	2495831..2497222 (-)	1392	WP_258613850.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40826.72 Da
• Isoelectric Point: 6.5709

>NTDB_id=627814 LOY28_RS11350 WP_086946288.1 2491772..2492881(+) (pilU) [Pseudomonas sp. B21-017]
MEIDALLQILASRHGSDLYLSTGAPPSAKFDGVLKALTDQPFKPGEVAAIAASIMDAEQRLEFDRDLEMNLALSLPGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLNLPPVLLETVMLKQGLMLFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRGQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFGELKGIMEKSSEVGMQTFDGAL
FALFAEGAIDEAEALKHADSVNNLRLRLKMHAEATPGPHTPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=627814 LOY28_RS11350 WP_086946288.1 2491772..2492881(+) (pilU) [Pseudomonas sp. B21-017]
ATGGAAATCGATGCACTGTTGCAGATTCTGGCCAGCCGCCATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGACGGTGTGCTCAAAGCCCTGACCGATCAACCGTTCAAGCCGGGAGAAGTGGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGACCTGGAAATGAACCTGGCACTTTCCCTGCCCGGGGTCGGGCGT
TTCCGGCTCAATATCTTCAAGCAGCGCAATGATGTGTCCATCGTGGCGCGCAACATCAAGCTCGACATTCCACGCTTCGA
AGACCTCAACTTGCCACCGGTGCTGCTCGAAACCGTGATGCTCAAACAAGGGCTGATGCTGTTCGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCGGCGCTGATCGATTACCGCAACCGCCATAGCAGTGGCCATATCATCACTATCGAAGAC
CCGGTCGAGTTTATCCATCGGCACAAGAAGTCGATCGTCAATCAGCGCGAGGTCGGCGTCGATACCCGCAGTTTCCACGC
TGCGCTGAAAAACACGTTGCGCCAGGCACCGGACGTGGTGTTGATCGGCGAAATCCGTGACCGCGAGACTATGGAACATG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTGATTTCCACGTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGCGTG
ATTAACTTCTTTCCCGAAGAGCGCCGGGGGCAGTTGCTCAACGACCTGGGCAACAACCTCAAAGCGTTTGTGTCCCAGCG
TCTGGTGCGTACCCGCGACGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCACACCAACCATCGGTGACCTGATCC
GGCGCAATGAGTTTGGCGAACTCAAGGGGATCATGGAGAAGTCTTCAGAGGTGGGGATGCAGACGTTTGACGGGGCGCTG
TTCGCGCTGTTTGCCGAGGGCGCAATTGATGAGGCAGAGGCGTTGAAACATGCTGATTCGGTGAACAATCTGCGCTTGCG
CCTGAAAATGCACGCCGAGGCCACACCCGGCCCCCATACGCCGCCGGGTGAATGGGGGTTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.62	99.729	0.545
pilU	Acinetobacter baylyi ADP1	52.528	96.477	0.507
pilU	Vibrio cholerae strain A1552	50.857	94.851	0.482
pilT	Pseudomonas stutzeri DSM 10701	40.988	93.225	0.382
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Vibrio cholerae strain A1552	41.441	90.244	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	90.244	0.374
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.528	91.87	0.363