Detailed information NTG0627785

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY36_RS11485
• Genome accession: NZ_CP087193
• Coordinates: 2536721..2537830 (+)
• Length: 369 a.a.
• NCBI ID: WP_258673583.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2531721..2542830

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY36_RS11470 (LOY36_11460)	-	2532592..2533527 (+)	936	WP_258673581.1	sugar kinase	-
LOY36_RS11475 (LOY36_11465)	-	2533723..2533998 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
LOY36_RS11480 (LOY36_11470)	-	2534065..2536602 (-)	2538	WP_258673582.1	PAS domain S-box protein	-
LOY36_RS11485 (LOY36_11475)	pilU	2536721..2537830 (+)	1110	WP_258673583.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY36_RS11490 (LOY36_11480)	-	2537957..2539912 (-)	1956	WP_258673586.1	acetoacetate--CoA ligase	-
LOY36_RS11495 (LOY36_11485)	hbdH	2540122..2540895 (-)	774	WP_258673587.1	3-hydroxybutyrate dehydrogenase	-
LOY36_RS11500 (LOY36_11490)	-	2540903..2542294 (-)	1392	WP_258673589.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40817.62 Da
• Isoelectric Point: 6.5709

>NTDB_id=627785 LOY36_RS11485 WP_258673583.1 2536721..2537830(+) (pilU) [Pseudomonas sp. B21-019]
MEIDALLQTLASRNGSDLYLSTGAPPSAKFDGVLKHLTDQPFKPGEVAAIAASIMDAEQRLEFDRDLEMNLALSLPGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLNLPAVLLESVMLKQGLMLFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRAQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFGELKGIMEKSSEAGMQTFDGAL
FALFAEGAIDEAEALKHADSVNNLRLRLKMHAEATPAPHTPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=627785 LOY36_RS11485 WP_258673583.1 2536721..2537830(+) (pilU) [Pseudomonas sp. B21-019]
ATGGAAATCGATGCACTGCTGCAGACTCTGGCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGATGGTGTGCTCAAACACTTGACCGATCAACCGTTCAAGCCGGGAGAAGTGGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGACCTGGAAATGAACCTGGCACTTTCCCTGCCCGGGGTCGGGCGT
TTCCGGCTCAATATCTTCAAACAGCGCAATGACGTGTCGATTGTGGCACGCAACATCAAACTCGACATTCCACGCTTCGA
AGATCTCAACTTGCCAGCGGTGCTGCTCGAATCCGTGATGCTCAAACAAGGGCTGATGCTGTTCGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGTTGGCGGCGCTGATCGATTACCGCAACCGCCATAGCAGCGGTCATATCATCACTATTGAAGAC
CCGGTCGAGTTTATCCATCGGCACAAGAAGTCGATCGTCAATCAGCGCGAGGTTGGCGTCGATACCCGCAGTTTCCATGC
GGCGCTGAAAAACACGTTGCGCCAGGCGCCGGACGTGGTGTTGATCGGCGAAATCCGCGACCGCGAGACTATGGAACACG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTGATTTCCACGTTGCATGCGCACAACGCCAATCAGGCGCTGGATCGGGTG
ATCAATTTCTTTCCCGAAGAACGCCGGGCACAACTGCTCAATGACTTGGGTAACAACCTCAAGGCGTTTGTGTCCCAGCG
TCTGGTGCGTACCCGCGACGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCACACCAACCATCGGTGACCTGATCC
GGCGCAATGAGTTTGGCGAACTCAAGGGGATCATGGAGAAGTCTTCAGAGGCAGGGATGCAGACGTTTGACGGGGCGCTG
TTCGCGCTGTTTGCCGAGGGTGCGATCGATGAGGCAGAGGCGTTGAAACATGCGGATTCTGTGAACAACTTGCGCTTGCG
CCTGAAAATGCACGCCGAGGCCACACCCGCCCCCCATACGCCGCCGGGTGAATGGGGGCTAATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.348	99.729	0.542
pilU	Acinetobacter baylyi ADP1	52.247	96.477	0.504
pilU	Vibrio cholerae strain A1552	50.286	94.851	0.477
pilT	Pseudomonas stutzeri DSM 10701	40.988	93.225	0.382
pilT	Pseudomonas aeruginosa PAK	40.988	93.225	0.382
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36