Detailed information NTG0627756

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY31_RS15835
• Genome accession: NZ_CP087192
• Coordinates: 3549395..3550504 (-)
• Length: 369 a.a.
• NCBI ID: WP_258711817.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-021
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3544395..3555504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY31_RS15820 (LOY31_15805)	-	3545197..3546588 (+)	1392	WP_007952055.1	GntP family permease	-
LOY31_RS15825 (LOY31_15810)	hbdH	3546596..3547369 (+)	774	WP_085603292.1	3-hydroxybutyrate dehydrogenase	-
LOY31_RS15830 (LOY31_15815)	-	3547433..3549388 (+)	1956	WP_258711816.1	acetoacetate--CoA ligase	-
LOY31_RS15835 (LOY31_15820)	pilU	3549395..3550504 (-)	1110	WP_258711817.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY31_RS15840 (LOY31_15825)	-	3550632..3553169 (+)	2538	WP_085603285.1	PAS domain-containing protein	-
LOY31_RS15845 (LOY31_15830)	-	3553220..3553495 (+)	276	WP_007952044.1	peptidylprolyl isomerase	-
LOY31_RS15850 (LOY31_15835)	-	3553621..3554553 (-)	933	WP_085603283.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41074.99 Da
• Isoelectric Point: 6.8358

>NTDB_id=627756 LOY31_RS15835 WP_258711817.1 3549395..3550504(-) (pilU) [Pseudomonas sp. B21-021]
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLSEQPFKPGEVATVAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPSVLLESVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHGLGNHLKAFVSQRLVRTRDGQRRAAAEVMLGTPTIADLIRRNDFGELKTIMEKSAELGMQTFDAAL
FALVAEGVIDEDEALKNADSVNNLRLRLKLHADTMPSPPPSTGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=627756 LOY31_RS15835 WP_258711817.1 3549395..3550504(-) (pilU) [Pseudomonas sp. B21-021]
ATGGAAATCGATGCATTGCTCAAACAACTGGCGAGCCGCCACGGTTCGGATCTGTTCCTGTCCACGGGCGCACCGCCCAG
TGCGCGGTTCGACGGGGTGCTGACGCCCTTGAGCGAGCAGCCGTTCAAACCGGGCGAAGTGGCGACTGTCGCCGCCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCCCGCACGGGTATCGGGCGC
TTCCGGGTCAACATCTTCAAACAGCGCAACGACGTGTCGATCGTGATCCGCAACGTCAAACTCGACATTCCTCGATTCGA
AGACCTGAAACTGCCGTCGGTATTGCTCGAATCGGTGATGCTCAAGCAAGGCCTGATCCTGTTCGTCGGCGCCACCGACT
CGGGCAAATCCACGTCGCTGGCGGCACTGATCGATCATCGCAATCGCCACAGCAGCGGGCACATCATCACCATTGAAGAC
CCGGTGGAATACATCCATCGCCACAAGAAATCGATCATCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTTCACGC
AGCGCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAGACCATGGAACATG
CGCTGGCCTTCGCCGAAACCGGGCATCTGGTGCTGTCGACATTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGAAGAGCGCCGCCCGCAATTGCTGCATGGCTTGGGCAATCACCTCAAGGCGTTCGTCTCCCAGCG
TCTGGTACGAACCCGGGACGGTCAGCGACGCGCCGCCGCCGAGGTGATGCTCGGTACGCCGACGATTGCCGATCTGATCC
GGCGCAATGACTTTGGCGAGCTGAAAACCATCATGGAAAAATCGGCGGAACTGGGCATGCAGACCTTTGACGCTGCACTG
TTCGCGCTGGTAGCAGAAGGGGTGATCGATGAAGACGAGGCACTGAAAAATGCTGATTCGGTCAACAACCTGCGCCTGCG
TCTGAAGCTGCACGCCGACACCATGCCGTCCCCACCGCCCTCGACCGGCGAGTGGGGACTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	96.206	0.542
pilU	Acinetobacter baylyi ADP1	53.693	95.393	0.512
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.118	91.87	0.369
pilT	Pseudomonas aeruginosa PAK	39.244	93.225	0.366
pilT	Legionella pneumophila strain Lp02	39.82	90.515	0.36
pilT	Legionella pneumophila strain ERS1305867	39.82	90.515	0.36