Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LOY48_RS22035 Genome accession   NZ_CP087186
Coordinates   5009195..5010412 (+) Length   405 a.a.
NCBI ID   WP_252884847.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain B21-063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5004195..5015412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY48_RS22015 (LOY48_21980) - 5005659..5005958 (+) 300 WP_252878910.1 DUF2845 domain-containing protein -
  LOY48_RS22020 (LOY48_21985) - 5006194..5006547 (-) 354 WP_071173629.1 BON domain-containing protein -
  LOY48_RS22025 (LOY48_21990) pilA/pilAI 5006843..5007265 (-) 423 WP_041477820.1 pilin Machinery gene
  LOY48_RS22030 (LOY48_21995) pilB 5007492..5009192 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY48_RS22035 (LOY48_22000) pilC 5009195..5010412 (+) 1218 WP_252884847.1 type II secretion system F family protein Machinery gene
  LOY48_RS22040 (LOY48_22005) pilD 5010414..5011286 (+) 873 WP_258707211.1 A24 family peptidase Machinery gene
  LOY48_RS22045 (LOY48_22010) coaE 5011283..5011906 (+) 624 WP_258707212.1 dephospho-CoA kinase -
  LOY48_RS22050 (LOY48_22015) yacG 5011903..5012103 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  LOY48_RS22055 (LOY48_22020) - 5012111..5012326 (-) 216 WP_016773148.1 hypothetical protein -
  LOY48_RS22060 (LOY48_22025) - 5012392..5013081 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  LOY48_RS22065 (LOY48_22030) - 5013272..5013898 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  LOY48_RS22070 (LOY48_22035) - 5013895..5014422 (+) 528 WP_160768590.1 MOSC domain-containing protein -
  LOY48_RS22075 (LOY48_22040) - 5014474..5014647 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44071.77 Da        Isoelectric Point: 10.1214

>NTDB_id=627609 LOY48_RS22035 WP_252884847.1 5009195..5010412(+) (pilC) [Pseudomonas siliginis strain B21-063]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAVFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=627609 LOY48_RS22035 WP_252884847.1 5009195..5010412(+) (pilC) [Pseudomonas siliginis strain B21-063]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTGTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAATCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCACAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGTGTGGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAAAAAGCCATGACCTATCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCGGTGTTTTTCGGCACTCGC
CACGCGCTGAAGACATCGCAGGCCCTGCGTGACCGCCGCGATGCCTGGCTGTTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCACGTTTTGCCCGCACCCTGTCGACCACATTCGCAGCCGGTGTGCCGCTGGTGGAAGCGCTGG
ATTCGGTCGCCGGTGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAGCTCAATTTCTCAATGCGCAGCACCGGTGTGTTTCCGAACATGGCAGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.599

98.519

0.39