Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LOY48_RS22025 Genome accession   NZ_CP087186
Coordinates   5006843..5007265 (-) Length   140 a.a.
NCBI ID   WP_041477820.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain B21-063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5001843..5012265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY48_RS22010 (LOY48_21975) - 5002711..5005662 (+) 2952 WP_258707210.1 DUF748 domain-containing protein -
  LOY48_RS22015 (LOY48_21980) - 5005659..5005958 (+) 300 WP_252878910.1 DUF2845 domain-containing protein -
  LOY48_RS22020 (LOY48_21985) - 5006194..5006547 (-) 354 WP_071173629.1 BON domain-containing protein -
  LOY48_RS22025 (LOY48_21990) pilA/pilAI 5006843..5007265 (-) 423 WP_041477820.1 pilin Machinery gene
  LOY48_RS22030 (LOY48_21995) pilB 5007492..5009192 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY48_RS22035 (LOY48_22000) pilC 5009195..5010412 (+) 1218 WP_252884847.1 type II secretion system F family protein Machinery gene
  LOY48_RS22040 (LOY48_22005) pilD 5010414..5011286 (+) 873 WP_258707211.1 A24 family peptidase Machinery gene
  LOY48_RS22045 (LOY48_22010) coaE 5011283..5011906 (+) 624 WP_258707212.1 dephospho-CoA kinase -
  LOY48_RS22050 (LOY48_22015) yacG 5011903..5012103 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14144.32 Da        Isoelectric Point: 8.4918

>NTDB_id=627607 LOY48_RS22025 WP_041477820.1 5006843..5007265(-) (pilA/pilAI) [Pseudomonas siliginis strain B21-063]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPSLAAVKGDNVTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVLGKTITLTRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=627607 LOY48_RS22025 WP_041477820.1 5006843..5007265(-) (pilA/pilAI) [Pseudomonas siliginis strain B21-063]
ATGAAAAAACAACAAGGCTTTACATTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGCATTCTGGCGACTATCGCCCT
GCCGCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAAGCTTGGCGGCGGTGAAAGGCGATAACGTCACCACTACCTCCAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCAGACGGAGCTGGAACGATTGTATGTACGTTGGCCAACGCGCCAGCGCCTGTATTGGG
CAAAACCATCACGCTGACACGTACAGCTGCGGGTGGTTGGAGCTGTGGCACTACGGTAGCTCCTGACTATTCACCGAAAG
GCTGTAACGCTACTGGTACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.365

97.857

0.493
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  comP Acinetobacter baylyi ADP1

41.447

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila str. Paris

39.041

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

42.222

96.429

0.407
  pilA2 Legionella pneumophila strain ERS1305867

36.986

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

39.259

96.429

0.379