Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N1705_RS18800 Genome accession   NZ_CP104123
Coordinates   3899122..3900507 (+) Length   461 a.a.
NCBI ID   WP_219411723.1    Uniprot ID   -
Organism   Escherichia coli strain USDA-ARS-USMARC-49610     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3894122..3905507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1705_RS18775 (N1705_18775) aroP 3894670..3896043 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N1705_RS18780 (N1705_18780) ampE 3896086..3896940 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N1705_RS18785 (N1705_18785) ampD 3896937..3897488 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N1705_RS18790 (N1705_18790) nadC 3897576..3898469 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N1705_RS18795 (N1705_18795) pilA 3898672..3899112 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N1705_RS18800 (N1705_18800) pilB 3899122..3900507 (+) 1386 WP_219411723.1 type II secretion system protein GspE Machinery gene
  N1705_RS18805 (N1705_18805) hofC 3900497..3901699 (+) 1203 WP_000157234.1 protein transport protein HofC -
  N1705_RS18810 (N1705_18810) guaC 3901734..3902777 (-) 1044 WP_001217338.1 GMP reductase -
  N1705_RS18815 (N1705_18815) - 3902933..3902977 (-) 45 WP_120795372.1 protein YacM -
  N1705_RS18820 (N1705_18820) coaE 3903002..3903622 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  N1705_RS18825 (N1705_18825) zapD 3903622..3904365 (+) 744 WP_001194734.1 cell division protein ZapD -
  N1705_RS18830 (N1705_18830) yacG 3904375..3904572 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  N1705_RS18835 (N1705_18835) mutT 3904679..3905068 (-) 390 WP_219411724.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50532.23 Da        Isoelectric Point: 6.5784

>NTDB_id=627360 N1705_RS18800 WP_219411723.1 3899122..3900507(+) (pilB) [Escherichia coli strain USDA-ARS-USMARC-49610]
MNIPQLTALCLSYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=627360 N1705_RS18800 WP_219411723.1 3899122..3900507(+) (pilB) [Escherichia coli strain USDA-ARS-USMARC-49610]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGAGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCAGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Vibrio campbellii strain DS40M4

48.579

83.948

0.408
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.831

88.72

0.362