Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY55_RS26040 Genome accession   NZ_CP087176
Coordinates   5694637..5696337 (+) Length   566 a.a.
NCBI ID   WP_223522737.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-040     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5689637..5701337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY55_RS26020 (LOY55_25980) - 5689771..5692755 (+) 2985 WP_258667091.1 DUF748 domain-containing protein -
  LOY55_RS26025 (LOY55_25985) - 5692791..5693051 (+) 261 WP_109786361.1 DUF2845 domain-containing protein -
  LOY55_RS26030 (LOY55_25990) - 5693327..5693680 (-) 354 WP_077431250.1 BON domain-containing protein -
  LOY55_RS26035 (LOY55_25995) - 5693989..5694411 (-) 423 WP_109786282.1 pilin -
  LOY55_RS26040 (LOY55_26000) pilB 5694637..5696337 (+) 1701 WP_223522737.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY55_RS26045 (LOY55_26005) pilC 5696340..5697557 (+) 1218 WP_223522736.1 type II secretion system F family protein Machinery gene
  LOY55_RS26050 (LOY55_26010) pilD 5697559..5698431 (+) 873 WP_223522735.1 A24 family peptidase Machinery gene
  LOY55_RS26055 (LOY55_26015) coaE 5698428..5699051 (+) 624 WP_046028514.1 dephospho-CoA kinase -
  LOY55_RS26060 (LOY55_26020) yacG 5699048..5699248 (+) 201 WP_046028515.1 DNA gyrase inhibitor YacG -
  LOY55_RS26065 (LOY55_26025) - 5699252..5699467 (-) 216 WP_046028516.1 hypothetical protein -
  LOY55_RS26070 (LOY55_26030) - 5699531..5700220 (-) 690 WP_077431246.1 energy-coupling factor ABC transporter permease -
  LOY55_RS26075 (LOY55_26035) - 5700217..5700690 (-) 474 WP_077432188.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62740.68 Da        Isoelectric Point: 6.0089

>NTDB_id=627359 LOY55_RS26040 WP_223522737.1 5694637..5696337(+) (pilB) [Pseudomonas sp. B21-040]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNKISLVSYLVQNNLAKSRQIAEIASEHFGMALLDLYCLDKETQPKGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHASGLEDMADVDLDG
LDIESVDDNKKDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPTQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYESDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVTLIIAQRLARKLCNHCKQSIDVPRETLLKEGFPE
ERIGSFAIYEPVGCDQCNNGYKGRVGIYEVVKNTKDLQRLIMAEGNSLQIDSQMRKDGFNDLRTSGLLKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627359 LOY55_RS26040 WP_223522737.1 5694637..5696337(+) (pilB) [Pseudomonas sp. B21-040]
ATGAACGACATCGCCCTAAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGCTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGCAACAAGATTTCCCTGGTCAGTTACCTGGTACAGAACAATCTGGCGAAGAGTCGACAGATCG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTGGACCTCTATTGCCTGGACAAGGAAACCCAGCCCAAAGGACTG
GTCAGCGAAAAGCTGATCCGCCAGCACCATGCGCTGCCTCTCTGGCGACGCGGCAACAAGTTGTTCGTGGGGATTTCCGA
CCCGACCAATCATCAGGCGATCAATGACATCCAGTTCAGCACCGGGCTGAACACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAGAAATTCTTCGACAGCCATGCCAGCGGCCTGGAAGACATGGCAGATGTCGATCTCGACGGG
CTGGACATCGAGTCGGTCGATGACAACAAAAAGGACGCTATCGCCGGGCAGGATGCCGACGATGCGCCCGTCGTGCGTTT
CGTCCACAAAATGCTGCTCGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCCTATGAAAAAACATACCGGG
TGCGGATGCGCACCGACGGCATGTTGCGCGAAGTGGCCAAGCCGCCCACTCAATTGGCCAACCGCATTGCGGCGCGGCTC
AAAGTCATGGCCAGCCTCGACATTTCCGAGCGCCGCAAACCTCAGGACGGGCGGATAAAAATGCGCCTGTCCAAGAGCAA
GTCGATTGATTTCCGGGTCAATACCCTGCCGACGCTATGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATTGATGCCCTCGGCTACGAGTCCGACCAGAAAGACTTGTACATGGCCGCCCTGAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCCACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGCTTGAATATCCTCAACACCGTGGA
CATCAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GGCTGGATTTCGCCCAGGCGCTGCGTTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAAATCCGCGATCTG
GAAACCGCCGAAATCGCCATCAAAGCCGCGCAGACCGGGCATCTGGTGCTGTCCACCCTGCACACCAACAGCGCCGCCGA
AACCTTGACCCGTCTGCATAACATGGGCATTCAGGGCTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGTC
TGGCGCGCAAATTGTGCAACCACTGCAAGCAATCCATCGACGTCCCTCGTGAAACGCTGCTCAAGGAAGGTTTTCCCGAG
GAACGCATCGGCTCATTCGCGATCTATGAGCCGGTCGGTTGCGATCAGTGCAACAACGGCTACAAAGGGCGCGTCGGGAT
TTATGAAGTGGTGAAGAACACCAAGGACCTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGCAAATCGACAGCCAGA
TGCGCAAGGACGGTTTCAACGACTTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCGTCACTAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.615

99.47

0.583
  pilB Acinetobacter baumannii D1279779

58.333

99.647

0.581
  pilB Legionella pneumophila strain ERS1305867

56.637

99.823

0.565
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.454

97.35

0.491
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.486
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

36.691

98.233

0.36