Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N1705_RS18795 Genome accession   NZ_CP104123
Coordinates   3898672..3899112 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain USDA-ARS-USMARC-49610     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3893672..3904112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1705_RS18775 (N1705_18775) aroP 3894670..3896043 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N1705_RS18780 (N1705_18780) ampE 3896086..3896940 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N1705_RS18785 (N1705_18785) ampD 3896937..3897488 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N1705_RS18790 (N1705_18790) nadC 3897576..3898469 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N1705_RS18795 (N1705_18795) pilA 3898672..3899112 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N1705_RS18800 (N1705_18800) pilB 3899122..3900507 (+) 1386 WP_219411723.1 type II secretion system protein GspE Machinery gene
  N1705_RS18805 (N1705_18805) hofC 3900497..3901699 (+) 1203 WP_000157234.1 protein transport protein HofC -
  N1705_RS18810 (N1705_18810) guaC 3901734..3902777 (-) 1044 WP_001217338.1 GMP reductase -
  N1705_RS18815 (N1705_18815) - 3902933..3902977 (-) 45 WP_120795372.1 protein YacM -
  N1705_RS18820 (N1705_18820) coaE 3903002..3903622 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=627359 N1705_RS18795 WP_000360904.1 3898672..3899112(+) (pilA) [Escherichia coli strain USDA-ARS-USMARC-49610]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=627359 N1705_RS18795 WP_000360904.1 3898672..3899112(+) (pilA) [Escherichia coli strain USDA-ARS-USMARC-49610]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363