Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   SCD_RS17015 Genome accession   NC_022357
Coordinates   2429746..2430192 (+) Length   148 a.a.
NCBI ID   WP_009205393.1    Uniprot ID   S6AIV9
Organism   Sulfuricella denitrificans skB26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2424746..2435192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCD_RS11770 (SCD_00663) - 2424775..2426310 (-) 1536 WP_023506985.1 CYTH and CHAD domain-containing protein -
  SCD_RS11775 (SCD_00664) - 2426307..2426930 (-) 624 WP_009205387.1 ParA family protein -
  SCD_RS11780 (SCD_00665) sixA 2426927..2427379 (-) 453 WP_009205388.1 phosphohistidine phosphatase SixA -
  SCD_RS11785 (SCD_00666) - 2427480..2427728 (+) 249 WP_009205389.1 hypothetical protein -
  SCD_RS11790 (SCD_00667) - 2427728..2428546 (+) 819 WP_009205390.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SCD_RS11795 (SCD_00668) - 2428568..2429347 (+) 780 WP_009205391.1 FHA domain-containing protein -
  SCD_RS11800 (SCD_00669) - 2429414..2429638 (+) 225 WP_009205392.1 hypothetical protein -
  SCD_RS17015 (SCD_00670) pilE 2429746..2430192 (+) 447 WP_009205393.1 pilin Machinery gene
  SCD_RS11810 (SCD_00671) - 2430256..2432184 (+) 1929 WP_009205394.1 type IV pilus biogenesis/stability protein PilW -
  SCD_RS11815 (SCD_00672) - 2432347..2433234 (+) 888 WP_009205395.1 glycosyltransferase family 2 protein -
  SCD_RS11820 (SCD_00673) - 2433236..2434333 (+) 1098 WP_009205396.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15369.77 Da        Isoelectric Point: 9.0088

>NTDB_id=62203 SCD_RS17015 WP_009205393.1 2429746..2430192(+) (pilE) [Sulfuricella denitrificans skB26]
MNKVQQGFTLIELMIVIAIIGILAAVALPAYQDYMIRAKVSEAVLAASSGRTAVSETFSQKGTMALSQASMGVQTQDSKY
VASVTYTGTSPTVGDITVLTKTLTDLGGASNTTMILRGTGTVGTGSVAWQCGKGTMPAKYLPSSCKDF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=62203 SCD_RS17015 WP_009205393.1 2429746..2430192(+) (pilE) [Sulfuricella denitrificans skB26]
ATGAATAAAGTACAACAAGGTTTTACCCTGATCGAACTGATGATCGTTATCGCGATTATCGGCATTCTGGCTGCAGTGGC
ACTGCCGGCCTACCAGGACTACATGATCCGCGCCAAGGTTTCCGAGGCTGTGCTGGCAGCATCTTCCGGCCGTACTGCGG
TTTCTGAAACATTCTCCCAAAAAGGAACGATGGCACTATCGCAAGCTTCCATGGGTGTGCAAACGCAAGATTCTAAATAT
GTTGCTTCAGTAACGTACACGGGAACCAGTCCGACAGTCGGTGACATTACCGTACTTACCAAGACCCTCACCGACTTGGG
CGGCGCGTCAAATACCACCATGATTCTGCGCGGAACGGGAACCGTAGGTACCGGCTCGGTTGCGTGGCAGTGCGGCAAGG
GCACCATGCCCGCAAAGTACCTGCCGAGCTCCTGCAAAGACTTCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AIV9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.053

100

0.527
  pilE Neisseria gonorrhoeae MS11

40.116

100

0.466
  comP Acinetobacter baylyi ADP1

44.156

100

0.459
  pilE Neisseria gonorrhoeae strain FA1090

37.853

100

0.453
  pilA2 Legionella pneumophila str. Paris

45.833

97.297

0.446
  pilA2 Legionella pneumophila strain ERS1305867

44.138

97.973

0.432
  pilA Ralstonia pseudosolanacearum GMI1000

37.349

100

0.419
  pilA Haemophilus influenzae 86-028NP

40.132

100

0.412
  pilA/pilA1 Eikenella corrodens VA1

36.943

100

0.392
  pilA Pseudomonas aeruginosa PAK

35.714

100

0.372

Multiple sequence alignment