Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LK431_RS00840 Genome accession   NZ_CP085951
Coordinates   159814..161208 (+) Length   464 a.a.
NCBI ID   WP_111695393.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_1561     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 154814..166208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK431_RS00815 (LK431_00815) rsmE 154829..155566 (-) 738 WP_111695394.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LK431_RS00820 (LK431_00820) lnt 155616..157145 (-) 1530 WP_111695739.1 apolipoprotein N-acyltransferase -
  LK431_RS00825 (LK431_00825) corC 157168..158067 (-) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  LK431_RS00830 (LK431_00830) ampD 158699..159253 (-) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LK431_RS00835 (LK431_00835) pilA 159368..159817 (+) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LK431_RS00840 (LK431_00840) pilB 159814..161208 (+) 1395 WP_111695393.1 GspE/PulE family protein Machinery gene
  LK431_RS00845 (LK431_00845) pilC 161205..162425 (+) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  LK431_RS00850 (LK431_00850) pilD 162422..163114 (+) 693 WP_041175383.1 prepilin peptidase Machinery gene
  LK431_RS00855 (LK431_00855) rho 163168..164430 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  LK431_RS00860 (LK431_00860) metJ 164678..164995 (+) 318 WP_021035707.1 met regulon transcriptional regulator MetJ -
  LK431_RS00865 (LK431_00865) cueR 165009..165395 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  LK431_RS00870 (LK431_00870) - 165472..165678 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  LK431_RS00875 (LK431_00875) - 165752..165958 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52911.37 Da        Isoelectric Point: 5.6520

>NTDB_id=621536 LK431_RS00840 WP_111695393.1 159814..161208(+) (pilB) [Haemophilus influenzae strain FDAARGOS_1561]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=621536 LK431_RS00840 WP_111695393.1 159814..161208(+) (pilB) [Haemophilus influenzae strain FDAARGOS_1561]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGTAATCAGCAACAACAGTCTCTGCTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTTTGGT
TAGGTGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.336

100

0.963
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.018

98.276

0.56
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.373
  pilF Thermus thermophilus HB27

35.611

100

0.371