Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LK431_RS00835 Genome accession   NZ_CP085951
Coordinates   159368..159817 (+) Length   149 a.a.
NCBI ID   WP_021035703.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_1561     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 154368..164817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK431_RS00815 (LK431_00815) rsmE 154829..155566 (-) 738 WP_111695394.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LK431_RS00820 (LK431_00820) lnt 155616..157145 (-) 1530 WP_111695739.1 apolipoprotein N-acyltransferase -
  LK431_RS00825 (LK431_00825) corC 157168..158067 (-) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  LK431_RS00830 (LK431_00830) ampD 158699..159253 (-) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LK431_RS00835 (LK431_00835) pilA 159368..159817 (+) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LK431_RS00840 (LK431_00840) pilB 159814..161208 (+) 1395 WP_111695393.1 GspE/PulE family protein Machinery gene
  LK431_RS00845 (LK431_00845) pilC 161205..162425 (+) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  LK431_RS00850 (LK431_00850) pilD 162422..163114 (+) 693 WP_041175383.1 prepilin peptidase Machinery gene
  LK431_RS00855 (LK431_00855) rho 163168..164430 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15478.84 Da        Isoelectric Point: 8.4623

>NTDB_id=621535 LK431_RS00835 WP_021035703.1 159368..159817(+) (pilA) [Haemophilus influenzae strain FDAARGOS_1561]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTSCTGGKNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANIEYILQATGNAAAGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=621535 LK431_RS00835 WP_021035703.1 159368..159817(+) (pilA) [Haemophilus influenzae strain FDAARGOS_1561]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACCATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAGCTGTACAGGGGGAAAAAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTTATCACTCAATCAGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATAGAATATATTTTGCAAGCTACAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

97.315

100

0.973
  pilA Haemophilus influenzae 86-028NP

93.289

100

0.933
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

41.727

93.289

0.389
  pilA Vibrio campbellii strain DS40M4

40.426

94.631

0.383