Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LIS66_RS22765 Genome accession   NZ_CP085885
Coordinates   5145721..5146938 (+) Length   405 a.a.
NCBI ID   WP_065615667.1    Uniprot ID   A0A423NHN1
Organism   Pseudomonas sp. HN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5140721..5151938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS66_RS22745 (LIS66_22745) - 5142134..5142433 (+) 300 WP_227973084.1 DUF2845 domain-containing protein -
  LIS66_RS22750 (LIS66_22750) - 5142701..5143054 (-) 354 WP_064364127.1 BON domain-containing protein -
  LIS66_RS22755 (LIS66_22755) - 5143354..5143791 (-) 438 WP_227973085.1 pilin -
  LIS66_RS22760 (LIS66_22760) pilB 5144018..5145718 (+) 1701 WP_042610478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LIS66_RS22765 (LIS66_22765) pilC 5145721..5146938 (+) 1218 WP_065615667.1 type II secretion system F family protein Machinery gene
  LIS66_RS22770 (LIS66_22770) pilD 5146940..5147812 (+) 873 WP_227973086.1 prepilin peptidase Machinery gene
  LIS66_RS22775 (LIS66_22775) coaE 5147809..5148432 (+) 624 WP_227973087.1 dephospho-CoA kinase -
  LIS66_RS22780 (LIS66_22780) yacG 5148429..5148629 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  LIS66_RS22785 (LIS66_22785) - 5148679..5149368 (-) 690 WP_083353590.1 energy-coupling factor ABC transporter permease -
  LIS66_RS22790 (LIS66_22790) - 5149558..5150184 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  LIS66_RS22795 (LIS66_22795) - 5150181..5150708 (+) 528 WP_042610484.1 MOSC domain-containing protein -
  LIS66_RS22800 (LIS66_22800) - 5150760..5150933 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44075.72 Da        Isoelectric Point: 10.1214

>NTDB_id=621133 LIS66_RS22765 WP_065615667.1 5145721..5146938(+) (pilC) [Pseudomonas sp. HN2]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATSLRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=621133 LIS66_RS22765 WP_065615667.1 5145721..5146938(+) (pilC) [Pseudomonas sp. HN2]
ATGGCGGTCAAGGCAGCAAAAGTCAGCGTTTACGCCTGGGAAGGCACGGATCGCAAAGGCAGCAAAGTCAGCGGCGAGTT
GAGTGGGCAGAATCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CCTCCCTGCTGAGCTTCGGCAAACGCATCAAGCCGCAGGACATCGCCTTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAACTCGTCGACGA
GGTCAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCACGTCATTGCGCAAAAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCTGGCGAGCAGTCCGGTGCCCTCGATACCTTGCTCGAACGCGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCGATGACTTACCCTACTGCCGTGGTGCTGGTGGCGGCAGTGGTGACCGGTAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCCGAATTGCCGGGCTTCACTCTGATGA
TCATCAGCCTGTCGGAGTTCATGCAGCAGTGGTGGTGGGCGATTCTCTTTGCCTTGGTCGCGGCGTTTTTCGGCACTCGC
CACGCGCTGAAGACTTCGCAGGCCCTGCGCGACCGTCGCGATGCCTGGCTACTGAAGCTGCCGCTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTTGCACGTTTTGCCCGCACCTTGTCGACTACATTCGCAGCCGGTGTGCCGTTGGTGGAAGCGCTCG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGTGCGGTGCTGCGGGTCCGGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTCTCGATGCGCAGCACCGGTGTATTCCCCAACATGGCGGTACAAATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTTGCCAGTTTCTATGAGGCCGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTTATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A423NHN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

39.348

98.519

0.388