Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LIS66_RS22760 Genome accession   NZ_CP085885
Coordinates   5144018..5145718 (+) Length   566 a.a.
NCBI ID   WP_042610478.1    Uniprot ID   -
Organism   Pseudomonas sp. HN2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5139018..5150718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS66_RS22740 (LIS66_22740) - 5139186..5142137 (+) 2952 WP_227973083.1 DUF748 domain-containing protein -
  LIS66_RS22745 (LIS66_22745) - 5142134..5142433 (+) 300 WP_227973084.1 DUF2845 domain-containing protein -
  LIS66_RS22750 (LIS66_22750) - 5142701..5143054 (-) 354 WP_064364127.1 BON domain-containing protein -
  LIS66_RS22755 (LIS66_22755) - 5143354..5143791 (-) 438 WP_227973085.1 pilin -
  LIS66_RS22760 (LIS66_22760) pilB 5144018..5145718 (+) 1701 WP_042610478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LIS66_RS22765 (LIS66_22765) pilC 5145721..5146938 (+) 1218 WP_065615667.1 type II secretion system F family protein Machinery gene
  LIS66_RS22770 (LIS66_22770) pilD 5146940..5147812 (+) 873 WP_227973086.1 prepilin peptidase Machinery gene
  LIS66_RS22775 (LIS66_22775) coaE 5147809..5148432 (+) 624 WP_227973087.1 dephospho-CoA kinase -
  LIS66_RS22780 (LIS66_22780) yacG 5148429..5148629 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  LIS66_RS22785 (LIS66_22785) - 5148679..5149368 (-) 690 WP_083353590.1 energy-coupling factor ABC transporter permease -
  LIS66_RS22790 (LIS66_22790) - 5149558..5150184 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  LIS66_RS22795 (LIS66_22795) - 5150181..5150708 (+) 528 WP_042610484.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62597.63 Da        Isoelectric Point: 6.0450

>NTDB_id=621132 LIS66_RS22760 WP_042610478.1 5144018..5145718(+) (pilB) [Pseudomonas sp. HN2]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=621132 LIS66_RS22760 WP_042610478.1 5144018..5145718(+) (pilB) [Pseudomonas sp. HN2]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAATTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGTGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATTGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTCAATTGCCTCGACAAAGAGACCCAGCCCAAGGGCCTG
GTTAGCGAAAAACTGGTTCGCCAGCACTGCGCCCTGCCCCTGTGGCGACGTGGCAACAAATTGTTCGTGGGTCTATCCGA
CCCGAGCAATCAGCAGGCAATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCTTCGGGCCTGGAAGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATCGATGACAACAAGCAGGACGTTATCGGCGGCCTCGATGCGGACGATGCTCCAGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAGAAAAACTACCGGG
TACGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATCGCCGCACGTTTG
AAGGTCATGGCCAGCCTCGATATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCTTGGGTTACGAGGCCGAACAAAAAGCCTTGTACCTCGCTGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGGCCCACCGGTTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGCCAGG
GGCTGGATTTCGCTCAGGCCCTGCGTTCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCAGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAGATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.567
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

94.523

0.369