Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   LE165_RS09495 Genome accession   NZ_CP084434
Coordinates   2005302..2005727 (+) Length   141 a.a.
NCBI ID   WP_267263077.1    Uniprot ID   -
Organism   Staphylococcus lugdunensis strain RMLUG2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1999497..2041078 2005302..2005727 within 0


Gene organization within MGE regions


Location: 1999497..2041078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LE165_RS09450 (LE165_09465) - 1999497..2000879 (-) 1383 WP_267263070.1 recombinase family protein -
  LE165_RS09455 (LE165_09470) - 2000937..2001761 (-) 825 WP_267263071.1 DUF5067 domain-containing protein -
  LE165_RS09460 (LE165_09475) - 2001761..2002240 (-) 480 WP_267263072.1 ImmA/IrrE family metallo-endopeptidase -
  LE165_RS09465 (LE165_09480) - 2002254..2002574 (-) 321 WP_267263073.1 helix-turn-helix transcriptional regulator -
  LE165_RS09470 (LE165_09485) - 2002738..2002983 (+) 246 WP_267263074.1 helix-turn-helix transcriptional regulator -
  LE165_RS09475 (LE165_09490) - 2003003..2003707 (+) 705 WP_267263075.1 Bro-N domain-containing protein -
  LE165_RS09480 (LE165_09495) - 2003897..2004076 (+) 180 WP_267263076.1 hypothetical protein -
  LE165_RS09485 (LE165_09500) - 2004145..2004630 (+) 486 WP_241448634.1 siphovirus Gp157 family protein -
  LE165_RS09490 (LE165_09505) - 2004631..2005305 (+) 675 WP_012991002.1 ERF family protein -
  LE165_RS09495 (LE165_09510) ssbA 2005302..2005727 (+) 426 WP_267263077.1 single-stranded DNA-binding protein Machinery gene
  LE165_RS09500 (LE165_09515) - 2005742..2006416 (+) 675 WP_267263078.1 putative HNHc nuclease -
  LE165_RS09505 (LE165_09520) - 2006409..2007215 (+) 807 WP_267263079.1 phage replisome organizer N-terminal domain-containing protein -
  LE165_RS09510 (LE165_09525) - 2007215..2007574 (+) 360 WP_267263080.1 hypothetical protein -
  LE165_RS09515 (LE165_09530) - 2007567..2008802 (+) 1236 WP_267263081.1 DnaB helicase C-terminal domain-containing protein -
  LE165_RS09520 (LE165_09535) - 2008799..2009026 (+) 228 WP_267263082.1 hypothetical protein -
  LE165_RS09525 (LE165_09540) - 2009016..2009258 (+) 243 WP_062078553.1 hypothetical protein -
  LE165_RS09530 (LE165_09545) - 2009267..2009674 (+) 408 WP_267263083.1 RusA family crossover junction endodeoxyribonuclease -
  LE165_RS09535 (LE165_09550) - 2009671..2009985 (+) 315 WP_014533256.1 hypothetical protein -
  LE165_RS09540 (LE165_09555) - 2009988..2010239 (+) 252 WP_012990993.1 hypothetical protein -
  LE165_RS09545 (LE165_09560) - 2010245..2010667 (+) 423 WP_267263084.1 DUF3310 domain-containing protein -
  LE165_RS09550 (LE165_09565) - 2010671..2011069 (+) 399 WP_267263085.1 hypothetical protein -
  LE165_RS09555 (LE165_09570) - 2011062..2011340 (+) 279 WP_267263086.1 hypothetical protein -
  LE165_RS09560 (LE165_09575) - 2011503..2011739 (+) 237 WP_267263087.1 hypothetical protein -
  LE165_RS09565 (LE165_09580) - 2011736..2011864 (+) 129 Protein_1840 DUF1024 family protein -
  LE165_RS09570 (LE165_09585) - 2011884..2012204 (+) 321 WP_062078560.1 MazG-like family protein -
  LE165_RS09575 (LE165_09590) - 2012304..2012627 (+) 324 WP_241448666.1 DUF1523 family protein -
  LE165_RS09580 (LE165_09595) - 2012635..2012784 (+) 150 WP_241448645.1 DUF1381 domain-containing protein -
  LE165_RS09585 (LE165_09600) rinB 2012777..2012959 (+) 183 WP_241448646.1 transcriptional activator RinB -
  LE165_RS09590 (LE165_09605) - 2012956..2013105 (+) 150 WP_012990987.1 hypothetical protein -
  LE165_RS09595 (LE165_09610) - 2013102..2013518 (+) 417 WP_012990986.1 hypothetical protein -
  LE165_RS09600 (LE165_09615) - 2013821..2014240 (+) 420 WP_241448647.1 helix-turn-helix domain-containing protein -
  LE165_RS09605 (LE165_09620) - 2014227..2015489 (+) 1263 WP_241448648.1 PBSX family phage terminase large subunit -
  LE165_RS09610 (LE165_09625) - 2015490..2016926 (+) 1437 WP_267263088.1 phage portal protein -
  LE165_RS09615 (LE165_09630) - 2016871..2017863 (+) 993 WP_267263089.1 phage head morphogenesis protein -
  LE165_RS09620 (LE165_09635) - 2017960..2018556 (+) 597 WP_062078570.1 phage scaffolding protein -
  LE165_RS09625 (LE165_09640) - 2018578..2019402 (+) 825 WP_267263090.1 N4-gp56 family major capsid protein -
  LE165_RS09630 (LE165_09645) - 2019419..2019700 (+) 282 WP_150907584.1 Rho termination factor N-terminal domain-containing protein -
  LE165_RS09635 (LE165_09650) - 2019700..2020011 (+) 312 WP_150907582.1 phage head-tail connector protein -
  LE165_RS09640 (LE165_09655) - 2020004..2020339 (+) 336 WP_062078578.1 phage head closure protein -
  LE165_RS09645 (LE165_09660) - 2020326..2020739 (+) 414 WP_150907580.1 HK97-gp10 family putative phage morphogenesis protein -
  LE165_RS09650 (LE165_09665) - 2020752..2021174 (+) 423 WP_062078582.1 DUF3168 domain-containing protein -
  LE165_RS09655 (LE165_09670) - 2021178..2021723 (+) 546 WP_062078584.1 hypothetical protein -
  LE165_RS09660 (LE165_09675) - 2021787..2022290 (+) 504 WP_267263091.1 tail assembly chaperone -
  LE165_RS09665 (LE165_09680) - 2022338..2022625 (+) 288 WP_062078588.1 hypothetical protein -
  LE165_RS09670 (LE165_09685) - 2022629..2025724 (+) 3096 WP_267263092.1 terminase -
  LE165_RS09675 (LE165_09690) - 2025737..2026666 (+) 930 WP_150907576.1 phage tail domain-containing protein -
  LE165_RS09680 (LE165_09695) - 2026678..2028534 (+) 1857 WP_150907575.1 SGNH/GDSL hydrolase family protein -
  LE165_RS09685 (LE165_09700) - 2028546..2031206 (+) 2661 WP_267263093.1 peptidase G2 autoproteolytic cleavage domain-containing protein -
  LE165_RS09690 (LE165_09705) - 2031206..2032735 (+) 1530 WP_267263094.1 BppU family phage baseplate upper protein -
  LE165_RS09695 (LE165_09710) - 2032740..2033075 (+) 336 WP_062078600.1 hypothetical protein -
  LE165_RS09700 (LE165_09715) - 2033077..2033220 (+) 144 WP_062078602.1 XkdX family protein -
  LE165_RS09705 (LE165_09720) - 2033252..2033551 (+) 300 WP_062078604.1 DUF2951 family protein -
  LE165_RS09710 (LE165_09725) - 2033689..2035569 (+) 1881 WP_267263095.1 glucosaminidase domain-containing protein -
  LE165_RS09715 (LE165_09730) - 2035580..2038147 (+) 2568 WP_267263096.1 BppU family phage baseplate upper protein -
  LE165_RS09720 (LE165_09735) - 2038204..2038638 (+) 435 WP_267263097.1 phage holin -
  LE165_RS09725 (LE165_09740) - 2038638..2040074 (+) 1437 WP_267263098.1 SH3 domain-containing protein -
  LE165_RS09730 (LE165_09745) - 2040231..2040473 (+) 243 WP_071561373.1 hypothetical protein -
  LE165_RS12805 - 2040473..2040886 (+) 414 WP_410483037.1 helix-turn-helix domain-containing protein -
  LE165_RS09740 (LE165_09755) - 2040941..2041078 (-) 138 Protein_1875 FAD-binding protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15945.56 Da        Isoelectric Point: 5.2281

>NTDB_id=611253 LE165_RS09495 WP_267263077.1 2005302..2005727(+) (ssbA) [Staphylococcus lugdunensis strain RMLUG2]
MINRVVLVGRLTKDPEFRTTPNGVSVATFTLAVNRTFTNAQGEREADFINVVVFRKQADNVNNYLNKGNLAGVDGRMQSR
SYENQEGKRVFVTEVVADSVQFLEPKNNKQSSNQPQQQNRQAQSDNNQFDNGLEEFSDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=611253 LE165_RS09495 WP_267263077.1 2005302..2005727(+) (ssbA) [Staphylococcus lugdunensis strain RMLUG2]
ATGATAAATAGAGTTGTATTAGTAGGAAGATTAACAAAAGACCCAGAATTCAGAACAACGCCAAATGGTGTAAGTGTTGC
TACCTTTACACTAGCAGTAAATCGTACATTCACAAATGCACAAGGAGAACGTGAGGCAGATTTTATTAACGTTGTAGTGT
TTAGAAAACAAGCAGATAACGTCAATAACTATTTAAACAAAGGTAATTTAGCCGGTGTTGACGGAAGAATGCAATCGCGA
AGTTATGAAAACCAGGAAGGTAAAAGAGTGTTTGTTACAGAAGTAGTAGCAGATAGTGTTCAGTTTTTAGAACCTAAAAA
TAACAAACAATCAAGCAACCAACCACAACAACAAAATAGACAAGCGCAAAGTGATAACAACCAATTTGATAATGGTTTAG
AAGAATTTTCAGATCTTCCGTTTTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

78.505

75.887

0.596

  ssb Latilactobacillus sakei subsp. sakei 23K

62.264

75.177

0.468

  ssbB Bacillus subtilis subsp. subtilis str. 168

55.752

80.142

0.447

  ssbA Streptococcus mutans UA159

41.844

100

0.418

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.844

100

0.418

  ssbB/cilA Streptococcus mitis NCTC 12261

39.716

100

0.397

  ssbB/cilA Streptococcus pneumoniae TIGR4

39.716

100

0.397

  ssbB/cilA Streptococcus pneumoniae Rx1

39.007

100

0.39

  ssbB/cilA Streptococcus pneumoniae D39

39.007

100

0.39

  ssbB/cilA Streptococcus pneumoniae R6

39.007

100

0.39

  ssbB/cilA Streptococcus mitis SK321

39.007

100

0.39