Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LEO74_RS02150 Genome accession   NZ_CP084352
Coordinates   473803..475044 (-) Length   413 a.a.
NCBI ID   WP_011707570.1    Uniprot ID   A0KPV8
Organism   Aeromonas hydrophila strain 71339     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468803..480044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO74_RS02120 (LEO74_02120) - 469644..470828 (+) 1185 WP_226031323.1 tetratricopeptide repeat protein -
  LEO74_RS02125 (LEO74_02125) mutT 470837..471250 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  LEO74_RS02130 (LEO74_02130) yacG 471311..471505 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  LEO74_RS02135 (LEO74_02135) zapD 471515..472237 (-) 723 WP_162498668.1 cell division protein ZapD -
  LEO74_RS02140 (LEO74_02140) coaE 472275..472889 (-) 615 WP_226031324.1 dephospho-CoA kinase -
  LEO74_RS02145 (LEO74_02145) pilD 472908..473780 (-) 873 WP_224481187.1 A24 family peptidase Machinery gene
  LEO74_RS02150 (LEO74_02150) pilC 473803..475044 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  LEO74_RS02155 (LEO74_02155) pilB 475160..476866 (-) 1707 WP_146061427.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LEO74_RS02160 (LEO74_02160) - 476873..477304 (-) 432 WP_041216993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LEO74_RS02165 (LEO74_02165) nadC 477627..478484 (-) 858 WP_224481186.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LEO74_RS02170 (LEO74_02170) - 478490..478960 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  LEO74_RS02175 (LEO74_02175) ampD 479108..479680 (+) 573 WP_171279817.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45422.58 Da        Isoelectric Point: 9.8637

>NTDB_id=610773 LEO74_RS02150 WP_011707570.1 473803..475044(-) (pilC) [Aeromonas hydrophila strain 71339]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=610773 LEO74_RS02150 WP_011707570.1 473803..475044(-) (pilC) [Aeromonas hydrophila strain 71339]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGTGGGGTGAACCGCAAGGGCCA
GAAAGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGTTATTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCCCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTTCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCAGC
CATGCGCGAGCTCATGGGGCAGATTGCCGCCGATGTGGAGACCGGCACCCCTATGTCCGAGGCGCTGCGCCGCCATCCCC
GTCATTTTGACGATCTCTACTGCGATCTGGTGGAGGCTGGCGAACAGTCCGGCGCTCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCTGAAGCACTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCACCATGGTCATCCTGGT
GGCCATTGTTGTTACCTCCATCCTGCTGCTGTTTGTCATTCCCCAGTTCGAGGACATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCATCTTCACCCAATTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATTTTTGGCGGCATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCATACTGACCAT
CCCGGTGGTTGGCATGATATTGCACAAGGCCGCCATGGCCCGCTTCGCCCGCACCCTCTCCACTACCTTTTCCGCCGGTA
TCCCGCTGGTGGATGCCTTGGTCTCTGCGGCAGGCGCCTCCGGCAACTATGTCTACCGTACTGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGATCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KPV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.348

96.126

0.446
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366