Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LEO74_RS02155 Genome accession   NZ_CP084352
Coordinates   475160..476866 (-) Length   568 a.a.
NCBI ID   WP_146061427.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 71339     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470160..481866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO74_RS02125 (LEO74_02125) mutT 470837..471250 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  LEO74_RS02130 (LEO74_02130) yacG 471311..471505 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  LEO74_RS02135 (LEO74_02135) zapD 471515..472237 (-) 723 WP_162498668.1 cell division protein ZapD -
  LEO74_RS02140 (LEO74_02140) coaE 472275..472889 (-) 615 WP_226031324.1 dephospho-CoA kinase -
  LEO74_RS02145 (LEO74_02145) pilD 472908..473780 (-) 873 WP_224481187.1 A24 family peptidase Machinery gene
  LEO74_RS02150 (LEO74_02150) pilC 473803..475044 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  LEO74_RS02155 (LEO74_02155) pilB 475160..476866 (-) 1707 WP_146061427.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LEO74_RS02160 (LEO74_02160) - 476873..477304 (-) 432 WP_041216993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LEO74_RS02165 (LEO74_02165) nadC 477627..478484 (-) 858 WP_224481186.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LEO74_RS02170 (LEO74_02170) - 478490..478960 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  LEO74_RS02175 (LEO74_02175) ampD 479108..479680 (+) 573 WP_171279817.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LEO74_RS02180 (LEO74_02180) pdhR 479956..480720 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62481.87 Da        Isoelectric Point: 5.4342

>NTDB_id=610774 LEO74_RS02155 WP_146061427.1 475160..476866(-) (pilB) [Aeromonas hydrophila strain 71339]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=610774 LEO74_RS02155 WP_146061427.1 475160..476866(-) (pilB) [Aeromonas hydrophila strain 71339]
ATGACCTCCAGCCCGAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCCCAGCGAAAACCTTTTGTGACCTTTCTCATCGAAAACGAAATCCTCGATAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATTGAAAAACATCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCTACCAATGTGTCGGCACTGGAGGACTTTGGCTTCAGCTTCGGGTTGCACACCGAGGCTCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATT
TCCGAACTGGAGGTGTCGGATGAGGGGTCGCGACTCGACGAGAGCGTCAATACAGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGATAGCAACTCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTCTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCTGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGACGTAGTCATGGTCGGTGAAATCCGGGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCCGAGGCTGAACTGCTTGAGCTGGGATTTACC
CAGCAGCAGCTGGCCGCAGGCTTGAGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375