Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8O61_RS16655 Genome accession   NZ_CP083804
Coordinates   3774852..3775994 (+) Length   380 a.a.
NCBI ID   WP_039007869.1    Uniprot ID   A0A514EFB3
Organism   Xanthomonas cerealis pv. cerealis strain ICMP 16317     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3769852..3780994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O61_RS16630 (K8O61_16635) - 3770064..3771251 (-) 1188 WP_039007864.1 cystathionine gamma-synthase -
  K8O61_RS16635 (K8O61_16640) - 3771334..3772704 (-) 1371 WP_039009295.1 pyridoxal-phosphate dependent enzyme -
  K8O61_RS16640 (K8O61_16645) - 3772905..3773129 (-) 225 WP_009575526.1 YdcH family protein -
  K8O61_RS16645 (K8O61_16650) - 3773468..3774364 (-) 897 WP_039007866.1 OmpA family protein -
  K8O61_RS16650 (K8O61_16655) - 3774361..3774753 (-) 393 WP_058195869.1 DUF4398 domain-containing protein -
  K8O61_RS16655 (K8O61_16660) pilU 3774852..3775994 (+) 1143 WP_039007869.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8O61_RS16660 (K8O61_16665) maiA 3776098..3776757 (-) 660 WP_058195870.1 maleylacetoacetate isomerase -
  K8O61_RS16665 (K8O61_16670) - 3776846..3777835 (-) 990 WP_039007873.1 fumarylacetoacetate hydrolase family protein -
  K8O61_RS16670 (K8O61_16675) - 3778211..3779572 (+) 1362 WP_047325324.1 DEAD/DEAH box helicase -
  K8O61_RS16675 (K8O61_16680) queD 3779706..3780068 (+) 363 WP_237655112.1 6-carboxytetrahydropterin synthase QueD -
  K8O61_RS16680 (K8O61_16685) - 3780332..3780703 (+) 372 WP_237655113.1 phasin family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42165.44 Da        Isoelectric Point: 6.3746

>NTDB_id=608000 K8O61_RS16655 WP_039007869.1 3774852..3775994(+) (pilU) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNMFKQRGEVGLVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFNNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKDKNERRRPATEVLLNTPMIRDLLRRGQVHEIKQAMEDSLEEGMETFDQCL
YRMVKQGQIEQEEALRAADSRDGLALKFRLSEGASGEHDPYADYDAGGGASSPRITHGFG

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=608000 K8O61_RS16655 WP_039007869.1 3774852..3775994(+) (pilU) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGGATATCGGCTACTTCTTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACGGGCGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTGAACATGGCCATCGCGCTGCAGGACGCCGGCCGC
TTCCGGGTCAACATGTTCAAGCAGCGCGGCGAGGTCGGCCTGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAACTGCACCTGCCGCAGGTGCTGAAGGACGTGATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACACTTTCAACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGACGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCAACGCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTTTCGCAGCG
GCTGGTCAAGGACAAGAACGAGCGCCGCCGCCCGGCCACCGAGGTGCTGCTGAACACGCCAATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGCAGGCGATGGAGGACTCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TACCGGATGGTCAAGCAGGGCCAGATCGAGCAGGAGGAAGCACTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGCCAGCGGCGAACACGACCCTTACGCCGACTACGACGCCGGCGGCGGCGCCAGCTCGCCGC
GCATCACCCACGGCTTCGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514EFB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

94.474

0.484
  pilU Vibrio cholerae strain A1552

49.153

93.158

0.458
  pilU Acinetobacter baylyi ADP1

48.459

93.947

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.832

100

0.439
  pilT Pseudomonas aeruginosa PAK

42.012

88.947

0.374
  pilT Acinetobacter baumannii D1279779

41.124

88.947

0.366
  pilT Acinetobacter nosocomialis M2

41.124

88.947

0.366
  pilT Acinetobacter baumannii strain A118

41.124

88.947

0.366
  pilT Legionella pneumophila strain Lp02

40.828

88.947

0.363
  pilT Legionella pneumophila strain ERS1305867

40.828

88.947

0.363