Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA321_RS19150 Genome accession   NZ_CP083625
Coordinates   4144893..4146032 (+) Length   379 a.a.
NCBI ID   WP_026457954.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1510     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4139893..4151032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA321_RS19120 (LA321_19120) - 4140083..4140463 (-) 381 WP_042069907.1 diacylglycerol kinase -
  LA321_RS19125 (LA321_19125) - 4140607..4141668 (+) 1062 WP_026456871.1 3-deoxy-7-phosphoheptulonate synthase -
  LA321_RS19130 (LA321_19130) - 4141749..4142534 (-) 786 WP_042069908.1 ATP-binding cassette domain-containing protein -
  LA321_RS19135 (LA321_19135) btuC 4142521..4143528 (-) 1008 WP_042028584.1 vitamin B12 ABC transporter permease BtuC -
  LA321_RS19140 (LA321_19140) - 4143631..4144362 (-) 732 WP_042069910.1 ABC transporter substrate-binding protein -
  LA321_RS19145 (LA321_19145) - 4144477..4144833 (+) 357 WP_005337656.1 DMT family protein -
  LA321_RS19150 (LA321_19150) pilU 4144893..4146032 (+) 1140 WP_026457954.1 type IVa pilus ATPase TapW Machinery gene
  LA321_RS19155 (LA321_19155) - 4146103..4146552 (-) 450 WP_042069911.1 isoprenylcysteine carboxylmethyltransferase family protein -
  LA321_RS19160 (LA321_19160) alr 4146716..4147942 (+) 1227 WP_042028580.1 alanine racemase -
  LA321_RS19165 (LA321_19165) - 4148136..4149080 (-) 945 WP_042069912.1 TerC family protein -
  LA321_RS19170 (LA321_19170) oppF 4149378..4150373 (-) 996 WP_042069914.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42308.37 Da        Isoelectric Point: 6.4287

>NTDB_id=606758 LA321_RS19150 WP_026457954.1 4144893..4146032(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1510]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLTETPLNPGDVASIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=606758 LA321_RS19150 WP_026457954.1 4144893..4146032(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1510]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGACCGAGACGCCCCTCAATCCGGGAGATGTGGCGAGCATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAAATCCCGCGCTTTGA
GGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGCCTGGTGCTGTTCGTCGGCGGTACCGGTT
CAGGCAAATCCACCTCACTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTAGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CCCTGGCCTTCTCGGAAACCGGCCACCTGGCTATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCGGAAGAACGGCGCCCGCAACTGCTCAACGATCTCGGCAACAACCTCAAGGCATTTGTCTCCCAACG
GCTGGTGAAGACCACGGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTTGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGTCTGAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGATAGCGCCCTG
TTCGATCTGGTAGTGGAAGGGGTGATCGATGAAGAAGAAGCGGTGAAGAACGCCGACTCGGCCAATAACCTGCGCCTCAA
GATCAAGCTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGATG
AGAAAGACGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364