Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA358_RS15020 Genome accession   NZ_CP083612
Coordinates   3248440..3249579 (+) Length   379 a.a.
NCBI ID   WP_224209924.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1536     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243440..3254579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA358_RS14990 (LA358_14990) - 3243629..3244009 (-) 381 WP_224209920.1 diacylglycerol kinase -
  LA358_RS14995 (LA358_14995) - 3244153..3245214 (+) 1062 WP_026456871.1 3-deoxy-7-phosphoheptulonate synthase -
  LA358_RS15000 (LA358_15000) - 3245296..3246081 (-) 786 WP_224209921.1 ATP-binding cassette domain-containing protein -
  LA358_RS15005 (LA358_15005) btuC 3246068..3247075 (-) 1008 WP_224209922.1 vitamin B12 ABC transporter permease BtuC -
  LA358_RS15010 (LA358_15010) - 3247178..3247909 (-) 732 WP_224209923.1 substrate-binding periplasmic protein -
  LA358_RS15015 (LA358_15015) - 3248024..3248380 (+) 357 WP_005337656.1 DMT family protein -
  LA358_RS15020 (LA358_15020) pilU 3248440..3249579 (+) 1140 WP_224209924.1 type IVa pilus ATPase TapW Machinery gene
  LA358_RS15025 (LA358_15025) - 3249655..3250104 (-) 450 WP_224209925.1 methyltransferase family protein -
  LA358_RS15030 (LA358_15030) alr 3250268..3251494 (+) 1227 WP_224209926.1 alanine racemase -
  LA358_RS15035 (LA358_15035) - 3251558..3252502 (-) 945 WP_201981390.1 TerC family protein -
  LA358_RS15040 (LA358_15040) oppF 3252799..3253794 (-) 996 WP_224209927.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42335.40 Da        Isoelectric Point: 6.4287

>NTDB_id=606640 LA358_RS15020 WP_224209924.1 3248440..3249579(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1536]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLTETPLNPGDVANIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=606640 LA358_RS15020 WP_224209924.1 3248440..3249579(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1536]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGACCGAGACGCCCCTCAATCCGGGAGATGTGGCGAACATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCGCGCTTTGA
GGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGCCTGGTGCTGTTCGTCGGGGGTACCGGTT
CAGGCAAATCCACCTCACTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTAGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGGGAAATTCGTGATCGCGAGACCATGGAGCATG
CCCTGGCCTTCTCGGAAACCGGTCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGGCGCCCGCAATTGCTCAACGATCTCGGCAACAACCTCAAGGCATTTGTCTCCCAACG
GCTGGTGAAAACCACAGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTTGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGCCTGAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGATGAAGAAGAAGCAGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAAGACGATCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364