Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LAZ82_RS01535 Genome accession   NZ_CP083589
Coordinates   331688..332833 (-) Length   381 a.a.
NCBI ID   WP_411909976.1    Uniprot ID   -
Organism   Herbaspirillum frisingense strain AU14559     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326688..337833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ82_RS01505 (LAZ82_01505) mutT 327221..327634 (+) 414 WP_102663292.1 8-oxo-dGTP diphosphatase MutT -
  LAZ82_RS01510 (LAZ82_01510) yacG 327722..327907 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  LAZ82_RS01515 (LAZ82_01515) zapD 327937..328692 (-) 756 WP_006713184.1 cell division protein ZapD -
  LAZ82_RS01520 (LAZ82_01520) coaE 328886..329506 (-) 621 WP_234295275.1 dephospho-CoA kinase -
  LAZ82_RS01525 (LAZ82_01525) pilD 329516..330379 (-) 864 WP_234295276.1 prepilin peptidase Machinery gene
  LAZ82_RS01530 (LAZ82_01530) - 330460..331701 (-) 1242 WP_234295277.1 type II secretion system F family protein -
  LAZ82_RS01535 (LAZ82_01535) pilF 331688..332833 (-) 1146 WP_411909976.1 GspE/PulE family protein Machinery gene
  LAZ82_RS01540 (LAZ82_01540) - 332946..334241 (-) 1296 WP_234295279.1 HlyC/CorC family transporter -
  LAZ82_RS01545 (LAZ82_01545) - 334356..335354 (-) 999 WP_326491462.1 glyoxylate/hydroxypyruvate reductase A -
  LAZ82_RS01550 (LAZ82_01550) - 335351..336208 (-) 858 WP_234295280.1 IclR family transcriptional regulator -
  LAZ82_RS01555 (LAZ82_01555) - 336477..337562 (+) 1086 WP_234295281.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 41268.02 Da        Isoelectric Point: 9.1752

>NTDB_id=606379 LAZ82_RS01535 WP_411909976.1 331688..332833(-) (pilF) [Herbaspirillum frisingense strain AU14559]
MPVMQAQTPAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHFFRVRLRVDGVLHELAQPPLTLRDKLATRLKILARL
DIAEKRLPQDGKMRLALDRRSIDLRVSTLPTQFGEKIVLRILDTAQASFSIAELGYEPAQQEALLQAIARPHGLVLMTGP
TGSGKTVSLYACLQRLNRPGVNIATAEDPVEINLPGINQVGINERAGLDFATALRAFLRQDPDVLMVGEIRDLETADIAV
KASQTGHLVLSTLHTNDAPSTLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAPGKPSTYVPQGCPACNQSGYRG
RTGIYQVMPVSAAMQALILAQAGTLELARQAQAEGIIDLRRAGLLKVLRGETSMGEILAGT

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=606379 LAZ82_RS01535 WP_411909976.1 331688..332833(-) (pilF) [Herbaspirillum frisingense strain AU14559]
ATGCCAGTCATGCAAGCTCAAACCCCGGCGGTGGCCGATGACGATGTCCCGGTAGTACGCCTGCTGCACAAGCTGCTGGC
CGACGCCGTCGGCATGGGCGCCTCAGACCTGCATTTTGAACCCTTCGAACATTTCTTCCGCGTACGCCTGCGCGTCGATG
GCGTGCTGCACGAACTGGCGCAGCCACCGCTGACACTGCGCGACAAGCTGGCTACCCGTCTGAAGATCCTGGCGCGTCTG
GACATCGCCGAAAAGCGCCTGCCCCAGGACGGCAAGATGCGCCTGGCACTGGACCGGCGCAGCATCGACCTGCGCGTCTC
CACCCTGCCCACCCAGTTCGGCGAAAAGATCGTCCTGCGCATTCTCGATACCGCCCAGGCCAGCTTCTCCATCGCCGAAC
TGGGCTATGAACCGGCCCAGCAGGAAGCGCTGCTGCAAGCCATCGCCCGACCGCATGGACTGGTGCTGATGACCGGCCCC
ACCGGCTCGGGCAAGACGGTCTCCCTGTACGCCTGCCTGCAGCGGCTCAACCGCCCCGGGGTCAACATCGCCACCGCCGA
GGACCCGGTGGAAATCAACCTGCCCGGCATCAACCAGGTCGGCATCAACGAACGCGCCGGGCTGGACTTCGCCACGGCGC
TGCGCGCCTTCCTGCGCCAGGACCCGGACGTGCTGATGGTGGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCGGTC
AAGGCGTCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACGCCCCCTCGACCCTCACGCGCCTGCTCAA
CATGGGGGTGCCGGCCTACAACATCGCTGCGTCGGTGAGCCTGATCGTGGCACAGCGGCTGGTACGCAAGCTGTGCCGCT
GCCGGCGTCCGGCGCCGGGCAAGCCATCCACCTATGTCCCGCAAGGCTGCCCGGCCTGCAACCAGAGCGGCTATCGCGGC
CGCACCGGCATCTACCAGGTGATGCCGGTCAGCGCCGCCATGCAGGCGCTGATCCTGGCCCAGGCCGGCACGCTGGAGCT
GGCACGCCAGGCGCAGGCCGAGGGCATCATCGACCTGCGCCGCGCCGGACTGCTCAAGGTCCTGCGCGGCGAAACCAGCA
TGGGAGAGATCCTTGCCGGCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(25-290)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.041

100

0.572
  pilB Vibrio parahaemolyticus RIMD 2210633

54.974

100

0.551
  pilB Acinetobacter baylyi ADP1

54.856

100

0.549
  pilB Vibrio campbellii strain DS40M4

54.712

100

0.549
  pilB Acinetobacter baumannii D1279779

54.427

100

0.549
  pilB Vibrio cholerae strain A1552

54.569

100

0.549
  pilB Legionella pneumophila strain ERS1305867

50.643

100

0.517
  pilB Haemophilus influenzae 86-028NP

46.791

98.163

0.459
  pilB Haemophilus influenzae Rd KW20

46.524

98.163

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

42.569

100

0.444
  pilF Thermus thermophilus HB27

42.597

100

0.43
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.228

100

0.428
  pilB Glaesserella parasuis strain SC1401

49.057

83.465

0.409
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.633

100

0.396