Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8O95_RS15340 Genome accession   NZ_CP082887
Coordinates   3321435..3322574 (+) Length   379 a.a.
NCBI ID   WP_026457954.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3316435..3327574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O95_RS15310 (K8O95_15280) - 3316591..3316971 (-) 381 WP_042069907.1 diacylglycerol kinase -
  K8O95_RS15315 (K8O95_15285) - 3317115..3318176 (+) 1062 WP_026456871.1 3-deoxy-7-phosphoheptulonate synthase -
  K8O95_RS15320 (K8O95_15290) - 3318291..3319076 (-) 786 WP_223378264.1 ATP-binding cassette domain-containing protein -
  K8O95_RS15325 (K8O95_15295) btuC 3319063..3320070 (-) 1008 WP_042028584.1 vitamin B12 ABC transporter permease BtuC -
  K8O95_RS15330 (K8O95_15300) - 3320173..3320904 (-) 732 WP_223378266.1 substrate-binding periplasmic protein -
  K8O95_RS15335 (K8O95_15305) - 3321019..3321375 (+) 357 WP_005337656.1 DMT family protein -
  K8O95_RS15340 (K8O95_15310) pilU 3321435..3322574 (+) 1140 WP_026457954.1 type IVa pilus ATPase TapW Machinery gene
  K8O95_RS15345 (K8O95_15315) - 3322650..3323099 (-) 450 WP_223378268.1 methyltransferase family protein -
  K8O95_RS15350 (K8O95_15320) alr 3323263..3324489 (+) 1227 WP_124240141.1 alanine racemase -
  K8O95_RS15355 (K8O95_15325) - 3324552..3325496 (-) 945 WP_223378270.1 TerC family protein -
  K8O95_RS15360 (K8O95_15330) oppF 3325794..3326789 (-) 996 WP_042028578.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42308.37 Da        Isoelectric Point: 6.4287

>NTDB_id=603377 K8O95_RS15340 WP_026457954.1 3321435..3322574(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1459]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLTETPLNPGDVASIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=603377 K8O95_RS15340 WP_026457954.1 3321435..3322574(+) (pilU) [Aeromonas enteropelogenes strain FDAARGOS_1459]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCTCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGACCGAGACGCCCCTCAATCCGGGAGATGTGGCGAGCATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCGCGCTTTGA
GGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGCCTGGTGCTGTTCGTCGGGGGTACCGGTT
CAGGCAAATCCACCTCGCTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTAGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCGGAAACCGGTCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCAGAAGAGCGGCGCCCACAACTGCTCAACGATCTCGGCAACAACCTCAAGGCATTTGTCTCCCAACG
GCTGGTGAAAACCACAGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTTGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGCCTGAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGATGAAGAAGAAGCAGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGTTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AAAAAGACGATCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364