Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   K8O86_RS10295 Genome accession   NZ_CP082850
Coordinates   2363353..2363787 (-) Length   144 a.a.
NCBI ID   WP_003632053.1    Uniprot ID   D3HRR3
Organism   Legionella longbeachae strain FDAARGOS_1481     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358353..2368787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O86_RS10270 (K8O86_10255) tsaB 2359378..2360028 (+) 651 WP_003632058.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  K8O86_RS10275 (K8O86_10260) - 2360038..2360229 (+) 192 WP_012979013.1 Trm112 family protein -
  K8O86_RS10280 (K8O86_10265) - 2360508..2361044 (+) 537 Protein_2024 IS5 family transposase -
  K8O86_RS10285 (K8O86_10270) - 2361217..2362617 (-) 1401 WP_003632056.1 APC family permease -
  K8O86_RS10290 (K8O86_10275) - 2362948..2363271 (-) 324 WP_003632054.1 BolA family transcriptional regulator -
  K8O86_RS10295 (K8O86_10280) pilA2 2363353..2363787 (-) 435 WP_003632053.1 pilin Machinery gene
  K8O86_RS10300 (K8O86_10285) - 2364080..2365324 (-) 1245 WP_003632052.1 6-phosphofructokinase -
  K8O86_RS10305 (K8O86_10290) letS 2365365..2368097 (-) 2733 WP_012979015.1 two-component system sensor histidine kinase LetS Regulator

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14827.97 Da        Isoelectric Point: 8.4860

>NTDB_id=603021 K8O86_RS10295 WP_003632053.1 2363353..2363787(-) (pilA2) [Legionella longbeachae strain FDAARGOS_1481]
MKQKGFTLIELMIVVAIIGVLAAIAIPAYQDYVVRARVTEGLSLASAAKTTVSENASSGAAQLSQGWVAPAQTPIVNNIA
VDNGTGVITITYTALAQGITLTLTPRSGGNALQAGAPPTDAITWTCTVNNPATNNRYVPANCRS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=603021 K8O86_RS10295 WP_003632053.1 2363353..2363787(-) (pilA2) [Legionella longbeachae strain FDAARGOS_1481]
ATGAAGCAAAAAGGTTTTACTTTAATTGAGTTGATGATAGTAGTGGCAATCATTGGTGTTCTCGCCGCAATAGCTATTCC
GGCCTATCAGGATTATGTAGTAAGAGCTCGCGTTACGGAAGGATTAAGCCTTGCCTCCGCAGCAAAAACTACAGTTTCTG
AAAACGCATCAAGCGGTGCTGCCCAATTAAGTCAAGGCTGGGTCGCACCTGCACAGACACCTATCGTTAACAATATCGCT
GTAGACAATGGGACTGGCGTAATTACAATAACTTACACTGCGTTAGCGCAGGGTATTACACTTACTTTAACGCCTCGATC
TGGCGGTAATGCTTTACAGGCCGGCGCTCCGCCAACCGATGCAATTACGTGGACTTGTACAGTTAATAATCCTGCTACAA
ATAATCGATATGTCCCTGCGAATTGCCGTTCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3HRR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

57.143

100

0.583
  pilA2 Legionella pneumophila str. Paris

57.143

100

0.583
  pilA Ralstonia pseudosolanacearum GMI1000

52.229

100

0.569
  comP Acinetobacter baylyi ADP1

47.945

100

0.486
  pilA/pilA1 Eikenella corrodens VA1

38.415

100

0.438
  pilA Vibrio parahaemolyticus RIMD 2210633

42.029

95.833

0.403
  pilE Neisseria gonorrhoeae MS11

36.25

100

0.403
  pilA Pseudomonas aeruginosa PAK

35.185

100

0.396
  pilA Acinetobacter baumannii strain A118

38.194

100

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.806

100

0.368