Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A9R16_RS03035 Genome accession   NZ_CP080624
Coordinates   607520..608689 (+) Length   389 a.a.
NCBI ID   WP_065971564.1    Uniprot ID   A0A1C2FZE1
Organism   Acidiferrobacter thiooxydans strain ZJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 602520..613689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9R16_RS03015 (A9R16_003035) - 602558..603715 (+) 1158 WP_065971568.1 acyl-CoA dehydrogenase family protein -
  A9R16_RS03020 (A9R16_003040) - 603712..606114 (+) 2403 WP_141689354.1 PAS domain S-box protein -
  A9R16_RS03025 (A9R16_003045) - 606138..606338 (+) 201 WP_065971566.1 DUF2905 family protein -
  A9R16_RS03030 (A9R16_003050) - 606395..607399 (+) 1005 WP_267254322.1 glycerate kinase -
  A9R16_RS03035 (A9R16_003055) pilU 607520..608689 (+) 1170 WP_065971564.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A9R16_RS03040 (A9R16_003060) - 608686..609192 (-) 507 WP_083996103.1 CBS domain-containing protein -
  A9R16_RS03045 (A9R16_003065) - 609237..610121 (-) 885 WP_065971562.1 fructosamine kinase family protein -
  A9R16_RS03050 (A9R16_003070) - 610413..610724 (+) 312 WP_065971561.1 hypothetical protein -
  A9R16_RS03055 (A9R16_003075) - 610891..611229 (-) 339 WP_065971560.1 HU family DNA-binding protein -
  A9R16_RS03060 (A9R16_003080) - 611430..611696 (+) 267 WP_065971559.1 FmdB family zinc ribbon protein -
  A9R16_RS03065 (A9R16_003085) - 611697..612338 (+) 642 WP_176716005.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 42963.22 Da        Isoelectric Point: 7.0064

>NTDB_id=594401 A9R16_RS03035 WP_065971564.1 607520..608689(+) (pilU) [Acidiferrobacter thiooxydans strain ZJ]
MTPEQATASLHGLLRFMLSKKASDLFLSVDFPPAIKLDGKMTPAGQQRLTPIHTKAFAHAIMNDKQRQEFEAEKECNFAI
SPPDIGRFRVNVFVQQGHVGLVLRTITSKIPTFDDLKLPAVLRDVCLTKRGLVILVGGTGSGKSTSLAAMIDYRNEHSHG
HIITIEDPVEYVHRHKNCLITHREVGADTHNWFAALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCMATLHANSA
NQALDRIVNFFPEERRNQLLMDLSLNARAIVSQRLIPRADGQGRAAAVEVLLNTPLVADLIFKGEVHSLKEVMAKSREQG
MQTFDQALFDLYENGAISYEDALRNADSVNELRLAIKLRGKGAPGTAQGSPEFRVMADPEPSATLTEAG

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=594401 A9R16_RS03035 WP_065971564.1 607520..608689(+) (pilU) [Acidiferrobacter thiooxydans strain ZJ]
ATGACACCCGAACAAGCCACCGCCAGCCTGCACGGCCTGCTGCGATTCATGTTGAGCAAGAAGGCTTCCGATCTGTTCCT
ATCCGTCGATTTTCCGCCCGCCATAAAGCTCGACGGCAAGATGACGCCGGCCGGCCAGCAGCGCCTTACGCCGATCCATA
CTAAGGCATTCGCCCATGCCATCATGAACGACAAGCAGCGCCAGGAATTCGAGGCCGAAAAGGAATGCAATTTCGCGATA
AGCCCGCCCGACATTGGCCGCTTTCGTGTCAACGTATTCGTTCAGCAGGGGCATGTGGGGTTGGTGCTGCGGACGATCAC
CAGCAAGATTCCGACGTTCGATGACCTGAAGCTCCCCGCGGTGTTGCGTGATGTGTGTCTTACGAAGCGCGGCCTCGTGA
TCCTGGTCGGCGGCACCGGTTCCGGGAAATCGACCTCGCTTGCGGCGATGATCGATTACCGCAATGAACACTCCCACGGC
CATATCATCACTATCGAAGATCCGGTGGAGTACGTGCATCGCCACAAGAACTGCCTCATTACCCATCGCGAGGTCGGGGC
CGACACGCATAACTGGTTCGCGGCGTTGAAGAACACGCTGCGCCAAGCCCCGGATGTGATTTTGATCGGCGAGATCCGGG
ATCGCGAGACGATGGAGTATGCGATCGCCTTTGCCGAGACCGGGCACCTGTGCATGGCCACCCTGCACGCCAACAGCGCC
AATCAGGCCCTGGACCGGATCGTGAATTTTTTCCCCGAGGAACGGCGCAATCAGCTGTTGATGGACCTGTCGTTGAATGC
GCGCGCCATCGTCTCCCAGCGGCTCATCCCGCGCGCCGACGGACAGGGGCGTGCGGCGGCGGTCGAGGTCTTGCTCAATA
CGCCCCTGGTGGCGGATCTCATCTTCAAGGGCGAGGTCCATTCTTTGAAGGAGGTGATGGCGAAGTCACGCGAGCAGGGC
ATGCAGACCTTCGATCAGGCGTTGTTCGACCTCTACGAGAACGGCGCCATCAGCTATGAGGATGCGCTGCGCAATGCCGA
TTCCGTCAACGAGCTGCGGCTTGCCATAAAGCTGCGTGGGAAGGGTGCCCCGGGCACGGCCCAGGGGTCCCCGGAATTTC
GTGTGATGGCCGACCCCGAGCCCTCCGCCACGCTGACTGAGGCGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2FZE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.717

88.946

0.558
  pilU Acinetobacter baylyi ADP1

57.429

89.974

0.517
  pilU Vibrio cholerae strain A1552

54.857

89.974

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.477

88.432

0.393
  pilT Pseudomonas aeruginosa PAK

43.413

85.861

0.373
  pilT Pseudomonas stutzeri DSM 10701

43.114

85.861

0.37
  pilT Acinetobacter baumannii D1279779

43.03

84.833

0.365
  pilT Acinetobacter baumannii strain A118

43.03

84.833

0.365
  pilT Acinetobacter baylyi ADP1

43.03

84.833

0.365
  pilT Acinetobacter nosocomialis M2

43.03

84.833

0.365