Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KY495_RS04210 Genome accession   NZ_CP080378
Coordinates   954841..956028 (-) Length   395 a.a.
NCBI ID   WP_219882503.1    Uniprot ID   -
Organism   Massilia sp. PAMC28688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949841..961028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KY495_RS04180 (KY495_04180) - 950565..951308 (+) 744 WP_229518493.1 ComF family protein -
  KY495_RS04185 (KY495_04185) trmL 951328..951798 (+) 471 WP_219882498.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  KY495_RS04190 (KY495_04190) - 951893..952201 (+) 309 WP_219882499.1 hypothetical protein -
  KY495_RS04195 (KY495_04195) - 952188..953666 (+) 1479 WP_219882500.1 S8 family serine peptidase -
  KY495_RS04200 (KY495_04200) - 953856..954329 (-) 474 WP_219882501.1 adenylyltransferase/cytidyltransferase family protein -
  KY495_RS24205 - 954464..954736 (-) 273 WP_307728240.1 M23 family metallopeptidase -
  KY495_RS04210 (KY495_04210) pilU 954841..956028 (-) 1188 WP_219882503.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KY495_RS04215 (KY495_04215) - 956183..957124 (+) 942 WP_219882504.1 hydrogen peroxide-inducible genes activator -
  KY495_RS04220 (KY495_04220) ubiA 957132..957986 (+) 855 WP_219882505.1 4-hydroxybenzoate octaprenyltransferase -
  KY495_RS04225 (KY495_04225) - 958062..958388 (+) 327 WP_219882506.1 YqjD family protein -
  KY495_RS04230 (KY495_04230) - 958392..958769 (+) 378 WP_219882507.1 phage holin family protein -
  KY495_RS04235 (KY495_04235) - 958762..959070 (+) 309 WP_219882508.1 hypothetical protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43987.27 Da        Isoelectric Point: 6.4020

>NTDB_id=592402 KY495_RS04210 WP_219882503.1 954841..956028(-) (pilU) [Massilia sp. PAMC28688]
MSTTFGPAEAQAYIHKLLTVMHHQGGSDLFISADFPPSMKHQGAMKPMSQQKLTGDVTRALALSLMNDKQTREFEQEMEC
NFAISLPNVCRFRVNVFVQQQCVGMVVRTIASEIPNFEKLDLPDVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NSAGHIITVEDPVEYVHKNKGCLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNSNQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTATIGEMILKGNFQGIKEIMHKS
RELGMCTFDQALYELYNKGHIGYDEAIRNADSANGLRLQIKLRGERKEPGVEAKNASADLSMAIEEEPEEEEPGH

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=592402 KY495_RS04210 WP_219882503.1 954841..956028(-) (pilU) [Massilia sp. PAMC28688]
ATGTCCACGACCTTTGGCCCGGCCGAAGCCCAGGCTTATATCCACAAGCTGCTCACCGTAATGCACCACCAGGGCGGCTC
CGACCTGTTCATCTCGGCCGACTTCCCACCCAGTATGAAGCACCAGGGCGCGATGAAGCCCATGAGCCAGCAAAAGCTCA
CCGGCGACGTCACGCGTGCCCTGGCGCTGTCGCTCATGAACGACAAGCAGACGCGCGAATTCGAGCAGGAGATGGAGTGC
AATTTCGCCATCTCGCTGCCCAATGTGTGCCGCTTTCGCGTCAACGTCTTTGTGCAGCAGCAGTGCGTCGGCATGGTGGT
GCGTACCATCGCGTCCGAAATTCCCAATTTTGAAAAGCTGGACCTGCCCGACGTGCTGAAAGACGTCATCATGACCAAGC
GCGGGCTGGTGCTGGTGGTGGGCGGTACCGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTACCGCAATGCC
AATTCGGCGGGCCACATCATTACCGTGGAAGACCCGGTGGAGTACGTCCACAAGAACAAGGGCTGCCTGATCACGCACCG
CGAAGTGGGCGTGGACACGCATTCATGGCACCACGCGCTCAAGAACACGCTGCGTCAGGCGCCGGACGTGATCCTGATCG
GCGAAATCCGCGATACCGAAACCATGGAGCACGCCATTGCGTTTGCCGAGACGGGCCACCTGTGCCTGGGTACCCTGCAC
GCCAACAATTCCAACCAGACCATGGACCGGATCATCAACTTCTTCCCGGAAGAGCGCCGCAACCAGCTGCTGATGGACCT
GTCGTCCAACCTGCGCGCCATTGTCTCGCAGCGCCTGGTGCGCACCGAAGACGGCAAGGGACGCAAGGCAGCCATTGAAA
TCCTGCTCAATACGGCCACCATCGGCGAGATGATCCTGAAGGGGAATTTCCAGGGCATCAAGGAGATCATGCACAAGTCG
CGCGAACTGGGCATGTGCACCTTCGACCAGGCGCTCTACGAGCTCTATAACAAGGGCCACATCGGCTACGATGAAGCGAT
CCGCAATGCAGATTCCGCCAACGGCTTGCGCCTCCAGATCAAGCTGCGGGGCGAGCGCAAGGAGCCCGGCGTGGAAGCGA
AAAATGCGTCGGCCGACCTGTCGATGGCGATCGAAGAGGAGCCGGAAGAGGAAGAGCCGGGCCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.201

95.949

0.539
  pilU Acinetobacter baylyi ADP1

53.672

89.62

0.481
  pilU Vibrio cholerae strain A1552

49.577

89.873

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

85.316

0.365
  pilT Acinetobacter baylyi ADP1

43.243

84.304

0.365
  pilT Acinetobacter baumannii D1279779

43.598

83.038

0.362
  pilT Acinetobacter nosocomialis M2

43.598

83.038

0.362
  pilT Acinetobacter baumannii strain A118

43.598

83.038

0.362
  pilT Legionella pneumophila strain Lp02

43.465

83.291

0.362
  pilT Legionella pneumophila strain ERS1305867

43.465

83.291

0.362