Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KW556_RS20270 Genome accession   NZ_CP079823
Coordinates   4331338..4332447 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain HD6454     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4326338..4337447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW556_RS20240 (KW556_20160) - 4326775..4327782 (-) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -
  KW556_RS20245 (KW556_20165) glnK 4328042..4328380 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  KW556_RS20250 (KW556_20170) arfB 4328582..4328998 (+) 417 WP_139430011.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  KW556_RS20255 (KW556_20175) - 4329045..4329536 (+) 492 WP_259584147.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  KW556_RS20260 (KW556_20180) - 4329897..4330322 (-) 426 WP_005340360.1 hypothetical protein -
  KW556_RS20265 (KW556_20185) yaaA 4330517..4331290 (-) 774 WP_064338818.1 peroxide stress protein YaaA -
  KW556_RS20270 (KW556_20190) pilU 4331338..4332447 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  KW556_RS20275 (KW556_20195) pilT 4332476..4333510 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  KW556_RS20280 (KW556_20200) - 4333550..4334251 (+) 702 WP_019445603.1 YggS family pyridoxal phosphate-dependent enzyme -
  KW556_RS20285 (KW556_20205) proC 4334393..4335217 (+) 825 WP_259584149.1 pyrroline-5-carboxylate reductase -
  KW556_RS20290 (KW556_20210) - 4335229..4335780 (+) 552 WP_005357946.1 YggT family protein -
  KW556_RS20295 (KW556_20215) yggU 4335780..4336082 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  KW556_RS20300 (KW556_20220) - 4336126..4336545 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=589803 KW556_RS20270 WP_005340356.1 4331338..4332447(-) (pilU) [Aeromonas veronii strain HD6454]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=589803 KW556_RS20270 WP_005340356.1 4331338..4332447(-) (pilU) [Aeromonas veronii strain HD6454]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
TCTCAAGGTAAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCTCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCTTGCAGTTCGCCGAGACAGGCCACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTGCCGCAAGACAAGCACCGCCAATTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATTGTCGCCCAGCA
GCTGGTGCCAAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCATCGCCTCAAGGAGGTAATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTTGCCCACGCTGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACAGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36