Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LPB400_RS08555 Genome accession   NZ_CP079818
Coordinates   1781367..1782497 (+) Length   376 a.a.
NCBI ID   WP_049328597.1    Uniprot ID   -
Organism   Neisseria perflava strain LPB0400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1774326..1787537 1781367..1782497 within 0


Gene organization within MGE regions


Location: 1774326..1787537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB400_RS08520 (LPB400_08530) rsmA 1774326..1775105 (-) 780 WP_107791931.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
  LPB400_RS08525 (LPB400_08535) - 1775194..1775793 (-) 600 WP_219088736.1 Maf family protein -
  LPB400_RS08530 (LPB400_08540) - 1775808..1776347 (-) 540 WP_070813868.1 gamma carbonic anhydrase family protein -
  LPB400_RS08535 (LPB400_08545) - 1776584..1777462 (+) 879 WP_219088738.1 DMT family transporter -
  LPB400_RS08540 (LPB400_08550) lepB 1777517..1778536 (-) 1020 WP_070584130.1 signal peptidase I -
  LPB400_RS08545 (LPB400_08555) lepA 1778536..1780329 (-) 1794 WP_002226499.1 translation elongation factor 4 -
  LPB400_RS08550 (LPB400_08560) - 1780462..1781163 (-) 702 WP_049328596.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LPB400_RS08555 (LPB400_08565) pilU 1781367..1782497 (+) 1131 WP_049328597.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPB400_RS08560 (LPB400_08570) - 1782571..1783977 (-) 1407 WP_070459864.1 dihydrolipoyl dehydrogenase -
  LPB400_RS08565 (LPB400_08575) - 1784231..1784968 (-) 738 WP_049348362.1 redoxin family protein -
  LPB400_RS08570 (LPB400_08580) - 1785157..1785942 (-) 786 WP_070459857.1 1-acylglycerol-3-phosphate O-acyltransferase -
  LPB400_RS08575 (LPB400_08585) mutM 1786007..1786834 (-) 828 WP_070460049.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  LPB400_RS08580 (LPB400_08590) - 1786878..1787537 (-) 660 WP_070459854.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42467.60 Da        Isoelectric Point: 5.6785

>NTDB_id=589656 LPB400_RS08555 WP_049328597.1 1781367..1782497(+) (pilU) [Neisseria perflava strain LPB0400]
MFNENTPDAKEELFAWLRHMNKYKGSDLFITTNFPPAMKVDGKIMRLTDEPLSAEKCMEIAFSIMSSKQIEEFSSTNECN
FAISLPDTSRFRVNAMVQRGATALVFRSITSNIPKFETLNLPPILKDIALRKRGLIIFVGGTGSGKSTSLASLIDYRNEN
SFDHIITIEDPIEFIHQHKNCIITQREIGVDTENWPIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHA
NSTNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPRESGRGRVAAVEVLLNSPIIADLIQKGEVHNIKEMMKKST
GMGMITFDQALYDLYENGDISYQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=589656 LPB400_RS08555 WP_049328597.1 1781367..1782497(+) (pilU) [Neisseria perflava strain LPB0400]
ATGTTTAACGAAAATACTCCGGATGCAAAAGAAGAATTGTTCGCTTGGCTACGCCATATGAACAAATACAAAGGCTCCGA
CCTTTTCATTACCACCAATTTCCCACCGGCCATGAAAGTGGACGGCAAAATCATGCGACTGACTGATGAGCCGCTGAGTG
CTGAAAAATGTATGGAAATCGCTTTTTCGATTATGTCTTCCAAACAAATCGAAGAATTTTCTTCCACCAACGAATGCAAC
TTCGCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTTAATGCAATGGTTCAACGCGGTGCAACTGCTTTGGTATTCCG
TTCCATTACCAGCAATATCCCGAAATTTGAAACGCTCAATCTACCCCCTATTCTGAAAGACATTGCCCTCAGAAAACGCG
GCCTGATTATTTTTGTCGGCGGTACAGGTTCGGGCAAGTCCACTTCCCTCGCCTCCCTTATCGACTATCGAAACGAAAAC
AGCTTCGACCACATTATTACCATCGAAGACCCGATTGAGTTTATCCACCAACACAAAAACTGCATCATCACCCAACGTGA
AATCGGTGTAGATACAGAAAATTGGCCGATTGCATTGAAAAATACCCTGCGCCAAGCGCCGGATGTTATCCTGATTGGTG
AAATCCGCGACCGCGAGACCATGGACTACGCCATTTCCTTCGCAGAAACCGGCCATTTGTGTATGGCAACACTGCACGCC
AACAGTACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTACCCAGTTGCTGACCGACTTATC
GCTCAACCTGCAAGCCTTTATTTCACAACGACTGATTCCCCGTGAAAGCGGACGGGGTCGTGTGGCGGCGGTCGAAGTTT
TGCTCAATTCGCCAATTATTGCCGACTTGATTCAAAAAGGCGAAGTCCACAACATCAAAGAAATGATGAAAAAATCAACC
GGCATGGGCATGATCACTTTTGACCAAGCCTTGTATGATTTGTATGAAAACGGCGATATCAGTTATCAAGACGCCATTAA
AAATGCCGACTCCGCTCATGATTTGCGCTTAGACATTCAATTACGAAGCCGTCGTGCGCAAAATGCCGGTCCGGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.227

93.617

0.564
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

52.925

95.479

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.243

88.564

0.383
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Legionella pneumophila strain Lp02

41.975

86.17

0.362
  pilT Legionella pneumophila strain ERS1305867

41.975

86.17

0.362
  pilT Acinetobacter baylyi ADP1

41.463

87.234

0.362