Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L37_RS18385 Genome accession   NZ_CP077399
Coordinates   3918994..3920133 (+) Length   379 a.a.
NCBI ID   WP_216992623.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1407     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3913994..3925133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L37_RS18355 (I6L37_18355) - 3914055..3914435 (-) 381 WP_052816484.1 diacylglycerol kinase -
  I6L37_RS18360 (I6L37_18360) - 3914577..3915641 (+) 1065 WP_010675196.1 3-deoxy-7-phosphoheptulonate synthase -
  I6L37_RS18365 (I6L37_18365) - 3915854..3916627 (-) 774 WP_216994206.1 ATP-binding cassette domain-containing protein -
  I6L37_RS18370 (I6L37_18370) btuC 3916614..3917621 (-) 1008 WP_216992622.1 vitamin B12 ABC transporter permease BtuC -
  I6L37_RS18375 (I6L37_18375) - 3917724..3918463 (-) 740 Protein_3544 substrate-binding periplasmic protein -
  I6L37_RS18380 (I6L37_18380) - 3918578..3918934 (+) 357 WP_005337656.1 DMT family protein -
  I6L37_RS18385 (I6L37_18385) pilU 3918994..3920133 (+) 1140 WP_216992623.1 type IVa pilus ATPase TapW Machinery gene
  I6L37_RS18390 (I6L37_18390) - 3920204..3920653 (-) 450 WP_216992624.1 isoprenylcysteine carboxylmethyltransferase family protein -
  I6L37_RS18395 (I6L37_18395) alr 3920817..3922043 (+) 1227 WP_042028580.1 alanine racemase -
  I6L37_RS18400 (I6L37_18400) - 3922106..3923050 (-) 945 WP_061475733.1 TerC family protein -
  I6L37_RS18405 (I6L37_18405) oppF 3923349..3924344 (-) 996 WP_216992625.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42363.41 Da        Isoelectric Point: 6.4287

>NTDB_id=581233 I6L37_RS18385 WP_216992623.1 3918994..3920133(+) (pilU) [Aeromonas sp. FDAARGOS 1407]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLTETPLNPGDVANIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSRALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=581233 I6L37_RS18385 WP_216992623.1 3918994..3920133(+) (pilU) [Aeromonas sp. FDAARGOS 1407]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGACCGAGACGCCCCTCAATCCGGGAGATGTGGCGAACATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCACGCAACATCAAGACCGAGATCCCGCGCTTTGA
AGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGCCTGGTGCTGTTCGTCGGGGGTACCGGCT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTAGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAATCAGCGCGAAGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACTCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CCCTGGCCTTCTCGGAAACCGGTCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGACGCCCGCAACTGCTCAACGATCTCGGCAACAACCTCAAGGCATTTGTCTCCCAACG
GCTGGTGAAGACCACGGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTTGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGTCTGAAGGAGGTGATGGAGAAATCCAGGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGATGAAGAAGAAGCGGTGAAAAACGCCGACTCGGCCAATAACCTGCGCCTCAA
GATCAAGTTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGATG
AGAAAGACGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364