Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L35_RS08985 Genome accession   NZ_CP077311
Coordinates   1903478..1904587 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1405     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1898478..1909587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L35_RS08955 (I6L35_08955) - 1899379..1899798 (-) 420 WP_113740088.1 DUF4426 domain-containing protein -
  I6L35_RS08960 (I6L35_08960) yggU 1899842..1900144 (-) 303 WP_021230942.1 DUF167 family protein YggU -
  I6L35_RS08965 (I6L35_08965) - 1900144..1900695 (-) 552 WP_124930250.1 YggT family protein -
  I6L35_RS08970 (I6L35_08970) proC 1900707..1901531 (-) 825 WP_216980068.1 pyrroline-5-carboxylate reductase -
  I6L35_RS08975 (I6L35_08975) - 1901674..1902375 (-) 702 WP_216980069.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L35_RS08980 (I6L35_08980) pilT 1902415..1903449 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  I6L35_RS08985 (I6L35_08985) pilU 1903478..1904587 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  I6L35_RS08990 (I6L35_08990) yaaA 1904635..1905408 (+) 774 WP_100646812.1 peroxide stress protein YaaA -
  I6L35_RS08995 (I6L35_08995) - 1905603..1906028 (+) 426 WP_005340360.1 hypothetical protein -
  I6L35_RS09000 (I6L35_09000) - 1906388..1906879 (-) 492 WP_216980070.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  I6L35_RS09005 (I6L35_09005) arfB 1906905..1907321 (-) 417 WP_216980071.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  I6L35_RS09010 (I6L35_09010) glnK 1907523..1907861 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  I6L35_RS09015 (I6L35_09015) - 1908120..1909127 (+) 1008 WP_216980262.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=580754 I6L35_RS08985 WP_005340356.1 1903478..1904587(+) (pilU) [Aeromonas sp. FDAARGOS 1405]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=580754 I6L35_RS08985 WP_005340356.1 1903478..1904587(+) (pilU) [Aeromonas sp. FDAARGOS 1405]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTAAATGGCCATCTGGTGTCGCTGGGGGGTGAGGCGCTCGACAAGAAGGGGGCACTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGTGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGCCATCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTACCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCTCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36