Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQY33_RS02000 Genome accession   NZ_CP076733
Coordinates   409570..409971 (-) Length   133 a.a.
NCBI ID   WP_284633965.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd2008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 404570..414971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQY33_RS01980 (KQY33_01980) coaE 404913..405512 (-) 600 WP_284633961.1 dephospho-CoA kinase -
  KQY33_RS01985 (KQY33_01985) pilD 405514..406392 (-) 879 WP_106338474.1 A24 family peptidase Machinery gene
  KQY33_RS01990 (KQY33_01990) pilC 406575..407801 (-) 1227 WP_284633963.1 type II secretion system F family protein Machinery gene
  KQY33_RS01995 (KQY33_01995) pilB 407867..409558 (-) 1692 WP_284633964.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KQY33_RS02000 (KQY33_02000) pilA 409570..409971 (-) 402 WP_284633965.1 pilin Machinery gene
  KQY33_RS02005 (KQY33_02005) ampD 410359..410937 (+) 579 WP_068969016.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KQY33_RS02010 (KQY33_02010) pdhR 411297..412070 (+) 774 Protein_374 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KQY33_RS02015 (KQY33_02015) aceE 412151..414811 (+) 2661 WP_068945542.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13781.88 Da        Isoelectric Point: 5.8012

>NTDB_id=577806 KQY33_RS02000 WP_284633965.1 409570..409971(-) (pilA) [Photobacterium damselae strain Pdd2008]
MKGQKGFTLIELMIVVAIIGVLSAIAIPAYKDYVAKSEVASAMATMKALVTPAELVIQETGKISGGLATLGTSAGANTLG
TIDIETATSAAKLTFTFNGGSLNDKKISLQRSDTGWSCQHDTDVDVKGCIQTN

Nucleotide


Download         Length: 402 bp        

>NTDB_id=577806 KQY33_RS02000 WP_284633965.1 409570..409971(-) (pilA) [Photobacterium damselae strain Pdd2008]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGATCATTGGTGTGTTATCTGCGATTGCGAT
TCCTGCTTACAAAGATTATGTCGCTAAGAGTGAAGTAGCATCAGCAATGGCAACAATGAAAGCTCTAGTAACACCCGCAG
AGTTAGTCATCCAAGAGACAGGGAAAATCAGTGGAGGGTTAGCGACTTTAGGTACATCAGCTGGAGCTAATACTTTAGGT
ACCATTGATATTGAGACAGCAACAAGTGCTGCAAAATTAACATTCACATTCAATGGTGGTTCATTAAACGATAAAAAAAT
ATCTCTCCAACGATCTGATACCGGCTGGTCATGTCAACATGATACAGATGTTGATGTAAAGGGGTGTATTCAGACTAACT
AA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

54.545

99.248

0.541
  pilA Pseudomonas aeruginosa PAK

45.946

100

0.511
  pilA Vibrio cholerae strain A1552

42.254

100

0.451
  pilA Vibrio cholerae C6706

42.254

100

0.451
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.254

100

0.451
  pilA Acinetobacter baumannii strain A118

43.066

100

0.444
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.971

100

0.399
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.098

100

0.391