Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   KI234_RS04160 Genome accession   NZ_CP076027
Coordinates   869169..869597 (+) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus sp. MZ7     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 859781..903952 869169..869597 within 0


Gene organization within MGE regions


Location: 859781..903952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI234_RS04075 (KI234_04075) sufU 859781..860245 (+) 465 WP_001010512.1 Fe-S cluster assembly sulfur transfer protein SufU -
  KI234_RS04080 (KI234_04080) sufB 860396..861793 (+) 1398 WP_001074405.1 Fe-S cluster assembly protein SufB -
  KI234_RS04085 (KI234_04085) - 861861..862910 (-) 1050 WP_001145730.1 tyrosine-type recombinase/integrase -
  KI234_RS04090 (KI234_04090) - 863107..863811 (-) 705 WP_017804779.1 type II toxin-antitoxin system PemK/MazF family toxin -
  KI234_RS04095 (KI234_04095) - 863843..864568 (-) 726 WP_000661437.1 PH domain-containing protein -
  KI234_RS04100 (KI234_04100) - 864596..865270 (-) 675 WP_000775187.1 ImmA/IrrE family metallo-endopeptidase -
  KI234_RS04105 (KI234_04105) - 865287..865619 (-) 333 WP_001055143.1 helix-turn-helix transcriptional regulator -
  KI234_RS04110 (KI234_04110) - 865882..866076 (+) 195 WP_000108122.1 helix-turn-helix transcriptional regulator -
  KI234_RS04115 (KI234_04115) - 866076..866843 (+) 768 WP_001002757.1 phage antirepressor Ant -
  KI234_RS04120 (KI234_04120) - 866844..867068 (+) 225 WP_000187184.1 hypothetical protein -
  KI234_RS04125 (KI234_04125) - 867108..867311 (+) 204 Protein_811 hypothetical protein -
  KI234_RS04130 (KI234_04130) - 867279..867524 (-) 246 WP_000122246.1 hypothetical protein -
  KI234_RS04135 (KI234_04135) - 867595..867816 (+) 222 WP_000977378.1 hypothetical protein -
  KI234_RS04140 (KI234_04140) - 867809..867970 (+) 162 WP_000066017.1 DUF1270 domain-containing protein -
  KI234_RS04145 (KI234_04145) - 868062..868322 (+) 261 WP_000291090.1 DUF1108 family protein -
  KI234_RS04150 (KI234_04150) - 868332..868553 (+) 222 WP_000815401.1 DUF2483 family protein -
  KI234_RS04155 (KI234_04155) - 868546..869169 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  KI234_RS04160 (KI234_04160) ssbA 869169..869597 (+) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  KI234_RS04165 (KI234_04165) - 869611..870285 (+) 675 WP_001124439.1 putative HNHc nuclease -
  KI234_RS04170 (KI234_04170) - 870282..870431 (+) 150 WP_001081076.1 hypothetical protein -
  KI234_RS04175 (KI234_04175) - 870424..870705 (-) 282 WP_000414755.1 hypothetical protein -
  KI234_RS04180 (KI234_04180) - 870771..871541 (+) 771 WP_000190253.1 conserved phage C-terminal domain-containing protein -
  KI234_RS04185 (KI234_04185) - 871551..872330 (+) 780 WP_000803062.1 ATP-binding protein -
  KI234_RS04190 (KI234_04190) - 872324..872482 (+) 159 WP_000256590.1 hypothetical protein -
  KI234_RS04195 (KI234_04195) - 872495..872716 (+) 222 WP_001123685.1 DUF3269 family protein -
  KI234_RS04200 (KI234_04200) - 872727..873131 (+) 405 WP_000049810.1 DUF1064 domain-containing protein -
  KI234_RS04205 (KI234_04205) - 873136..873321 (+) 186 WP_049317452.1 DUF3113 family protein -
  KI234_RS04210 (KI234_04210) - 873322..873678 (+) 357 WP_000029369.1 SA1788 family PVL leukocidin-associated protein -
  KI234_RS04215 (KI234_04215) - 873682..873924 (+) 243 WP_000131381.1 phi PVL orf 51-like protein -
  KI234_RS04220 (KI234_04220) - 873939..874190 (+) 252 WP_025174939.1 DUF1024 family protein -
  KI234_RS04225 (KI234_04225) - 874180..874353 (+) 174 WP_000028424.1 hypothetical protein -
  KI234_RS04230 (KI234_04230) - 874354..874635 (+) 282 WP_000454994.1 hypothetical protein -
  KI234_RS04235 (KI234_04235) - 874636..874797 (+) 162 WP_000889683.1 hypothetical protein -
  KI234_RS04240 (KI234_04240) - 874812..875345 (+) 534 WP_025920701.1 dUTP diphosphatase -
  KI234_RS04245 (KI234_04245) - 875382..875588 (+) 207 WP_000195827.1 DUF1381 domain-containing protein -
  KI234_RS04250 (KI234_04250) - 875585..875779 (+) 195 Protein_836 hypothetical protein -
  KI234_RS04255 (KI234_04255) - 875776..875979 (+) 204 WP_001072795.1 hypothetical protein -
  KI234_RS04260 (KI234_04260) - 875972..876208 (+) 237 WP_000608278.1 hypothetical protein -
  KI234_RS04265 (KI234_04265) - 876201..876374 (+) 174 WP_000595257.1 transcriptional activator RinB -
  KI234_RS04270 (KI234_04270) - 876375..876521 (+) 147 WP_000990005.1 hypothetical protein -
  KI234_RS04275 (KI234_04275) - 876545..876967 (+) 423 WP_000162701.1 RinA family phage transcriptional activator -
  KI234_RS04280 (KI234_04280) - 877155..877649 (+) 495 WP_001038244.1 terminase small subunit -
  KI234_RS04285 (KI234_04285) - 877652..878947 (+) 1296 WP_117172029.1 PBSX family phage terminase large subunit -
  KI234_RS04290 (KI234_04290) - 878958..880496 (+) 1539 WP_214958327.1 phage portal protein -
  KI234_RS04295 (KI234_04295) - 880503..881498 (+) 996 WP_001133041.1 minor capsid protein -
  KI234_RS04300 (KI234_04300) - 881571..881741 (+) 171 WP_061732709.1 hypothetical protein -
  KI234_RS04305 (KI234_04305) - 881876..882490 (+) 615 WP_000354305.1 DUF4355 domain-containing protein -
  KI234_RS04310 (KI234_04310) - 882504..883478 (+) 975 WP_000438511.1 phage major capsid protein -
  KI234_RS04315 (KI234_04315) - 883500..883787 (+) 288 WP_214958328.1 hypothetical protein -
  KI234_RS04320 (KI234_04320) - 883796..884128 (+) 333 WP_214958329.1 phage head-tail connector protein -
  KI234_RS04325 (KI234_04325) - 884125..884427 (+) 303 WP_001268312.1 hypothetical protein -
  KI234_RS04330 (KI234_04330) - 884427..884774 (+) 348 WP_001017815.1 HK97-gp10 family putative phage morphogenesis protein -
  KI234_RS04335 (KI234_04335) - 884786..885169 (+) 384 WP_000188642.1 hypothetical protein -
  KI234_RS04340 (KI234_04340) - 885188..885769 (+) 582 WP_000002577.1 phage major tail protein, TP901-1 family -
  KI234_RS04345 (KI234_04345) - 885831..886196 (+) 366 WP_001100161.1 tail assembly chaperone -
  KI234_RS04350 (KI234_04350) - 886226..886570 (+) 345 WP_000105584.1 hypothetical protein -
  KI234_RS04355 (KI234_04355) - 886587..890054 (+) 3468 WP_072472461.1 hypothetical protein -
  KI234_RS04360 (KI234_04360) - 890067..891014 (+) 948 WP_000350675.1 phage tail family protein -
  KI234_RS04365 (KI234_04365) - 891023..892921 (+) 1899 WP_000152718.1 SGNH/GDSL hydrolase family protein -
  KI234_RS04370 (KI234_04370) - 892936..894846 (+) 1911 WP_031928995.1 hypothetical protein -
  KI234_RS04375 (KI234_04375) - 894846..896669 (+) 1824 WP_072472460.1 phage baseplate upper protein -
  KI234_RS04380 (KI234_04380) - 896669..897046 (+) 378 WP_000705914.1 DUF2977 domain-containing protein -
  KI234_RS04385 (KI234_04385) - 897056..897229 (+) 174 WP_001790193.1 XkdX family protein -
  KI234_RS04390 (KI234_04390) - 897269..897568 (+) 300 WP_000466777.1 DUF2951 domain-containing protein -
  KI234_RS04395 (KI234_04395) - 897705..899603 (+) 1899 WP_214958330.1 glucosaminidase domain-containing protein -
  KI234_RS04400 (KI234_04400) - 899616..900854 (+) 1239 WP_069990338.1 BppU family phage baseplate upper protein -
  KI234_RS04405 (KI234_04405) - 900860..901255 (+) 396 WP_000398874.1 hypothetical protein -
  KI234_RS04410 (KI234_04410) - 901311..901586 (+) 276 WP_000351121.1 phage holin -
  KI234_RS04415 (KI234_04415) - 901573..902985 (+) 1413 WP_001141515.1 N-acetylmuramoyl-L-alanine amidase -
  KI234_RS04420 (KI234_04420) eta 903110..903952 (+) 843 WP_001065781.1 exfoliative toxin A -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=571453 KI234_RS04160 WP_000934385.1 869169..869597(+) (ssbA) [Staphylococcus sp. MZ7]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=571453 KI234_RS04160 WP_000934385.1 869169..869597(+) (ssbA) [Staphylococcus sp. MZ7]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394