Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   SAI4T8_RS10115 Genome accession   NC_020533
Coordinates   2018681..2019106 (-) Length   141 a.a.
NCBI ID   WP_000934384.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus ST228     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1984851..2024766 2018681..2019106 within 0


Gene organization within MGE regions


Location: 1984851..2024766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAI4T8_RS09860 scn 1984851..1985201 (-) 351 WP_000702263.1 complement inhibitor SCIN-A -
  SAI4T8_RS09865 - 1985712..1986047 (-) 336 Protein_1866 SH3 domain-containing protein -
  SAI4T8_RS09870 (SAI4T8_1014720) sak 1986698..1987189 (-) 492 WP_000920038.1 staphylokinase -
  SAI4T8_RS09875 (SAI4T8_1014730) - 1987380..1988135 (-) 756 WP_000861038.1 CHAP domain-containing protein -
  SAI4T8_RS09880 - 1988147..1988401 (-) 255 WP_000611512.1 phage holin -
  SAI4T8_RS09885 - 1988453..1988560 (+) 108 WP_031762631.1 hypothetical protein -
  SAI4T8_RS14590 pepG1 1988613..1988747 (-) 135 WP_000226108.1 type I toxin-antitoxin system toxin PepG1 -
  SAI4T8_RS09895 (SAI4T8_1014740) sea 1988898..1989671 (-) 774 WP_000750412.1 staphylococcal enterotoxin type A -
  SAI4T8_RS09900 - 1990044..1990418 (-) 375 WP_000340977.1 hypothetical protein -
  SAI4T8_RS09905 - 1990474..1990761 (-) 288 WP_001262621.1 hypothetical protein -
  SAI4T8_RS09910 - 1990807..1990959 (-) 153 WP_001000058.1 hypothetical protein -
  SAI4T8_RS09915 (SAI4T8_1014750) - 1990952..1994734 (-) 3783 WP_015445885.1 phage tail spike protein -
  SAI4T8_RS09920 (SAI4T8_1014760) - 1994750..1996234 (-) 1485 WP_000567390.1 phage tail domain-containing protein -
  SAI4T8_RS09925 (SAI4T8_1014770) - 1996231..2000760 (-) 4530 WP_015445887.1 phage tail tape measure protein -
  SAI4T8_RS15030 - 2000817..2000954 (-) 138 WP_001549167.1 hypothetical protein -
  SAI4T8_RS09935 - 2001005..2001355 (-) 351 WP_001096355.1 hypothetical protein -
  SAI4T8_RS14595 - 2001405..2001635 (-) 231 Protein_1881 Ig-like domain-containing protein -
  SAI4T8_RS09940 (SAI4T8_1014780) - 2001671..2002315 (-) 645 WP_000268741.1 major tail protein -
  SAI4T8_RS09945 - 2002316..2002723 (-) 408 WP_000565498.1 hypothetical protein -
  SAI4T8_RS09950 - 2002720..2003124 (-) 405 WP_000114225.1 HK97 gp10 family phage protein -
  SAI4T8_RS09955 - 2003121..2003483 (-) 363 WP_000755150.1 head-tail adaptor protein -
  SAI4T8_RS09960 - 2003467..2003751 (-) 285 WP_000150936.1 phage head-tail adapter protein -
  SAI4T8_RS09965 - 2003741..2004025 (-) 285 WP_000238236.1 hypothetical protein -
  SAI4T8_RS09970 (SAI4T8_1014790) - 2004045..2005190 (-) 1146 WP_000154555.1 phage major capsid protein -
  SAI4T8_RS09975 (SAI4T8_1014800) - 2005214..2005951 (-) 738 WP_000861914.1 head maturation protease, ClpP-related -
  SAI4T8_RS09980 (SAI4T8_1014810) - 2005935..2007122 (-) 1188 WP_000025274.1 phage portal protein -
  SAI4T8_RS09985 (SAI4T8_1014820) - 2007138..2008799 (-) 1662 WP_015445889.1 terminase large subunit -
  SAI4T8_RS09990 - 2008796..2009140 (-) 345 WP_000402904.1 hypothetical protein -
  SAI4T8_RS09995 - 2009270..2009569 (-) 300 WP_000988336.1 HNH endonuclease -
  SAI4T8_RS10000 - 2009801..2010217 (-) 417 WP_000590126.1 hypothetical protein -
  SAI4T8_RS10005 - 2010245..2010445 (-) 201 WP_000265041.1 DUF1514 family protein -
  SAI4T8_RS10010 - 2010445..2010579 (-) 135 Protein_1896 transcriptional regulator -
  SAI4T8_RS10015 - 2010586..2010789 (-) 204 WP_041174300.1 hypothetical protein -
  SAI4T8_RS10020 - 2010786..2010980 (-) 195 WP_000132920.1 hypothetical protein -
  SAI4T8_RS10025 - 2011035..2011181 (-) 147 Protein_1899 DUF1381 domain-containing protein -
  SAI4T8_RS10030 (SAI4T8_1014830) - 2011218..2011754 (-) 537 WP_000185680.1 dUTPase -
  SAI4T8_RS10035 - 2011747..2011995 (-) 249 WP_041174218.1 DUF1024 family protein -
  SAI4T8_RS10040 - 2011988..2012296 (-) 309 WP_001614813.1 hypothetical protein -
  SAI4T8_RS10045 - 2012293..2012640 (-) 348 WP_000979209.1 YopX family protein -
  SAI4T8_RS10050 - 2012637..2013038 (-) 402 WP_000695762.1 hypothetical protein -
  SAI4T8_RS10055 - 2013051..2013299 (-) 249 WP_012068339.1 SAV1978 family virulence-associated passenger protein -
  SAI4T8_RS10060 - 2013299..2013553 (-) 255 WP_000022735.1 DUF3310 domain-containing protein -
  SAI4T8_RS10065 - 2013639..2013824 (-) 186 WP_001187230.1 DUF3113 family protein -
  SAI4T8_RS10070 - 2013829..2014233 (-) 405 WP_031895320.1 DUF1064 domain-containing protein -
  SAI4T8_RS10075 - 2014230..2014655 (-) 426 WP_000451861.1 phage N-6-adenine-methyltransferase -
  SAI4T8_RS10080 - 2014665..2014886 (-) 222 WP_001123688.1 DUF3269 family protein -
  SAI4T8_RS10085 - 2014890..2015105 (-) 216 WP_001024405.1 hypothetical protein -
  SAI4T8_RS10090 (SAI4T8_1014840) - 2015102..2016343 (-) 1242 WP_015445891.1 DnaB helicase C-terminal domain-containing protein -
  SAI4T8_RS10095 - 2016340..2016696 (-) 357 WP_001132243.1 hypothetical protein -
  SAI4T8_RS10100 (SAI4T8_1014850) - 2016696..2017451 (-) 756 WP_001037657.1 DnaD domain protein -
  SAI4T8_RS10105 (SAI4T8_1014860) - 2017444..2018118 (-) 675 WP_015445893.1 putative HNHc nuclease -
  SAI4T8_RS10110 (SAI4T8_1014870) - 2018119..2018670 (-) 552 WP_001004506.1 NUMOD4 domain-containing protein -
  SAI4T8_RS10115 ssbA 2018681..2019106 (-) 426 WP_000934384.1 single-stranded DNA-binding protein Machinery gene
  SAI4T8_RS10120 (SAI4T8_1014880) - 2019106..2019729 (-) 624 WP_000139720.1 DUF1071 domain-containing protein -
  SAI4T8_RS10125 - 2019722..2019943 (-) 222 WP_000815403.1 DUF2483 family protein -
  SAI4T8_RS10130 - 2019953..2020213 (-) 261 WP_000291076.1 DUF1108 family protein -
  SAI4T8_RS10135 - 2020305..2020466 (-) 162 WP_000066021.1 DUF1270 family protein -
  SAI4T8_RS10140 - 2020478..2020741 (-) 264 WP_001124198.1 helix-turn-helix domain-containing protein -
  SAI4T8_RS15140 - 2020891..2020989 (-) 99 Protein_1923 hypothetical protein -
  SAI4T8_RS10150 - 2020986..2021312 (-) 327 WP_001025595.1 hypothetical protein -
  SAI4T8_RS10155 (SAI4T8_1014890) - 2021368..2022000 (+) 633 WP_001814660.1 hypothetical protein -
  SAI4T8_RS10160 - 2022015..2022155 (-) 141 WP_000939500.1 hypothetical protein -
  SAI4T8_RS10165 (SAI4T8_1014900) groL 2022790..2024406 (-) 1617 WP_000240642.1 chaperonin GroEL -
  SAI4T8_RS10170 groES 2024482..2024766 (-) 285 WP_000917289.1 co-chaperone GroES -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15784.48 Da        Isoelectric Point: 8.4826

>NTDB_id=56974 SAI4T8_RS10115 WP_000934384.1 2018681..2019106(-) (ssbA) [Staphylococcus aureus subsp. aureus ST228]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNNADSIEDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=56974 SAI4T8_RS10115 WP_000934384.1 2018681..2019106(-) (ssbA) [Staphylococcus aureus subsp. aureus ST228]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGATTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAACAACGCAG
ACTCTATAGAGGATCTTCCTTTTTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

73.729

83.688

0.617
  ssb Latilactobacillus sakei subsp. sakei 23K

50

100

0.603
  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

75.177

0.44
  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.844

100

0.418
  ssbA Streptococcus mutans UA159

46.087

81.56

0.376
  ssbB/cilA Streptococcus mitis NCTC 12261

44.915

83.688

0.376
  ssbB/cilA Streptococcus pneumoniae TIGR4

44.915

83.688

0.376
  ssbB/cilA Streptococcus pneumoniae Rx1

44.068

83.688

0.369
  ssbB/cilA Streptococcus pneumoniae D39

44.068

83.688

0.369
  ssbB/cilA Streptococcus pneumoniae R6

44.068

83.688

0.369
  ssbB/cilA Streptococcus mitis SK321

44.068

83.688

0.369

Multiple sequence alignment