Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   KIW23_RS10440 Genome accession   NZ_CP075561
Coordinates   2121701..2122639 (-) Length   312 a.a.
NCBI ID   WP_251314861.1    Uniprot ID   -
Organism   Floricoccus penangensis strain ML061-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2116701..2127639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIW23_RS10400 (KIW23_10415) - 2117144..2118079 (-) 936 WP_251314854.1 metal ABC transporter substrate-binding protein -
  KIW23_RS10405 (KIW23_10420) ispE 2118158..2119006 (-) 849 WP_251314855.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  KIW23_RS10410 (KIW23_10425) comGG 2119032..2119340 (-) 309 WP_251314856.1 competence type IV pilus minor pilin ComGG -
  KIW23_RS10415 (KIW23_10430) comGF 2119340..2119777 (-) 438 WP_251314857.1 competence type IV pilus minor pilin ComGF -
  KIW23_RS10420 (KIW23_10435) comGE 2119764..2120057 (-) 294 WP_251314858.1 competence type IV pilus minor pilin ComGE -
  KIW23_RS10425 (KIW23_10440) comGD 2120029..2120463 (-) 435 WP_083312879.1 competence type IV pilus minor pilin ComGD -
  KIW23_RS10430 (KIW23_10445) comYC 2120423..2120737 (-) 315 WP_070787377.1 competence type IV pilus major pilin ComGC Machinery gene
  KIW23_RS10435 (KIW23_10450) comYB 2120734..2121777 (-) 1044 WP_251314860.1 competence type IV pilus assembly protein ComGB Machinery gene
  KIW23_RS10440 (KIW23_10455) comGA/cglA/cilD 2121701..2122639 (-) 939 WP_251314861.1 competence type IV pilus ATPase ComGA Machinery gene
  KIW23_RS10445 (KIW23_10460) - 2122728..2123120 (-) 393 WP_233278814.1 DUF1033 family protein -
  KIW23_RS10450 (KIW23_10465) - 2123203..2123643 (-) 441 WP_251314862.1 CoA-binding protein -
  KIW23_RS10455 (KIW23_10470) polA 2123845..2126469 (-) 2625 WP_251314863.1 DNA polymerase I -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35672.06 Da        Isoelectric Point: 7.5477

>NTDB_id=569580 KIW23_RS10440 WP_251314861.1 2121701..2122639(-) (comGA/cglA/cilD) [Floricoccus penangensis strain ML061-4]
MVLEKAKIIIQLAIERSVHDIYFLPKENFYEIRYRSSKENRLHEKLSIEESLALISHFKFISGMNVGEKRRSQLGSCNYS
LDSVKKRLRLSTVGDVFGMESMVIRILYSEEDDAQFWFNGQVEIEKIINRRGLYLFSGPVGSGKTTLMYLLARLKFEGKQ
VITIEDPVEIIQKNFLQLQLNRAIGNDYDSLIKLSLRHMPDLVIVGEIRDEQTARAVIRASLTGYTVFSTIHAKSVGGAY
ERLLELGISKSEIENSVSAVIYQRLLVGKGLIDFSIGNIQNHRGEKWNEQLEKLFADGHISHDEKESEKIIT

Nucleotide


Download         Length: 939 bp        

>NTDB_id=569580 KIW23_RS10440 WP_251314861.1 2121701..2122639(-) (comGA/cglA/cilD) [Floricoccus penangensis strain ML061-4]
ATGGTGCTAGAAAAAGCAAAAATAATAATTCAATTAGCTATAGAAAGAAGTGTTCATGATATATACTTCTTACCTAAAGA
AAATTTTTATGAGATTAGATATAGATCATCCAAGGAAAATAGATTGCATGAAAAGCTTTCTATTGAAGAATCATTAGCAT
TAATTTCACACTTTAAATTTATCTCAGGTATGAATGTAGGTGAAAAGAGACGTTCGCAACTAGGATCTTGTAATTATAGT
CTCGATTCAGTAAAGAAAAGGTTACGTCTTTCAACTGTTGGTGATGTATTCGGAATGGAAAGTATGGTAATTCGAATTCT
CTATAGTGAGGAGGATGACGCACAATTTTGGTTTAATGGACAAGTAGAAATCGAAAAAATCATAAATAGACGTGGTCTCT
ATCTATTCTCAGGTCCTGTAGGGAGTGGGAAGACGACTCTGATGTACCTTTTAGCTAGATTAAAATTTGAAGGTAAGCAA
GTTATTACCATTGAAGATCCAGTTGAAATTATTCAAAAAAATTTTTTACAACTTCAATTAAATAGAGCGATAGGAAATGA
CTATGATAGTTTGATAAAACTATCTCTCAGACATATGCCTGATTTGGTTATTGTTGGAGAAATTAGAGATGAACAAACAG
CACGTGCGGTAATACGCGCTAGTTTGACTGGCTATACAGTTTTTTCAACAATTCATGCAAAGAGTGTTGGAGGTGCCTAT
GAAAGACTTTTAGAATTAGGAATAAGTAAATCAGAAATTGAAAATTCCGTATCAGCAGTTATTTATCAACGTCTTTTAGT
TGGTAAAGGCCTAATTGACTTTTCTATAGGTAATATACAAAATCATAGAGGGGAGAAATGGAATGAACAACTTGAAAAAT
TATTTGCAGATGGACATATCTCTCATGATGAGAAAGAATCCGAAAAAATTATCACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae R6

57.742

99.359

0.574
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

57.742

99.359

0.574
  comGA/cglA/cilD Streptococcus pneumoniae D39

57.742

99.359

0.574
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

57.742

99.359

0.574
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

57.742

99.359

0.574
  comYA Streptococcus mutans UA159

57.556

99.679

0.574
  comYA Streptococcus mutans UA140

57.556

99.679

0.574
  comYA Streptococcus gordonii str. Challis substr. CH1

57.419

99.359

0.571
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.949

99.679

0.558
  comGA Lactococcus lactis subsp. cremoris KW2

55.949

99.679

0.558