Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MKF35_RS12425 Genome accession   NZ_CP092629
Coordinates   2687389..2687853 (+) Length   154 a.a.
NCBI ID   WP_016264268.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2682389..2692853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS12400 yacG 2682559..2682759 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MKF35_RS12405 coaE 2682756..2683367 (-) 612 WP_047105677.1 dephospho-CoA kinase -
  MKF35_RS12410 pilD 2683364..2684236 (-) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MKF35_RS12415 pilC 2684237..2685454 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  MKF35_RS12420 pilB 2685458..2687158 (-) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKF35_RS12425 pilA 2687389..2687853 (+) 465 WP_016264268.1 pilin Machinery gene
  MKF35_RS12430 - 2687935..2689314 (+) 1380 WP_229027906.1 O-antigen ligase family protein -
  MKF35_RS12440 nadC 2689494..2690342 (-) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16089.44 Da        Isoelectric Point: 9.0145

>NTDB_id=567236 MKF35_RS12425 WP_016264268.1 2687389..2687853(+) (pilA) [Pseudomonas aeruginosa strain HU20]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKNPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=567236 MKF35_RS12425 WP_016264268.1 2687389..2687853(+) (pilA) [Pseudomonas aeruginosa strain HU20]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGAATCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCTAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.26

94.805

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

31.818

100

0.409
  pilA Vibrio cholerae C6706

36.943

100

0.377
  pilA Vibrio cholerae strain A1552

36.943

100

0.377
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.943

100

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.926

96.753

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37