Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KHW13_RS03565 Genome accession   NZ_CP074411
Coordinates   803525..804742 (-) Length   405 a.a.
NCBI ID   WP_096134052.1    Uniprot ID   -
Organism   Pseudomonas syringae strain Susan762     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 798525..809742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW13_RS25645 - 799829..800065 (-) 237 WP_327397062.1 DUF3094 family protein -
  KHW13_RS03535 (KHW13_03535) - 799950..800483 (-) 534 WP_213568008.1 MOSC domain-containing protein -
  KHW13_RS03540 (KHW13_03540) - 800480..801109 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  KHW13_RS03545 (KHW13_03545) - 801237..801698 (+) 462 WP_213568009.1 hypothetical protein -
  KHW13_RS03550 (KHW13_03550) yacG 801825..802034 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  KHW13_RS03555 (KHW13_03555) coaE 802031..802654 (-) 624 WP_004415092.1 dephospho-CoA kinase -
  KHW13_RS03560 (KHW13_03560) pilD 802651..803523 (-) 873 WP_096134051.1 A24 family peptidase Machinery gene
  KHW13_RS03565 (KHW13_03565) pilC 803525..804742 (-) 1218 WP_096134052.1 type II secretion system F family protein Machinery gene
  KHW13_RS03570 (KHW13_03570) pilB 804745..806439 (-) 1695 WP_096134053.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW13_RS03575 (KHW13_03575) - 806665..807060 (+) 396 WP_096134054.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KHW13_RS03580 (KHW13_03580) - 807248..808528 (+) 1281 WP_347256466.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44297.23 Da        Isoelectric Point: 10.1709

>NTDB_id=564711 KHW13_RS03565 WP_096134052.1 803525..804742(-) (pilC) [Pseudomonas syringae strain Susan762]
MASKAVKVSVYTWEGVDKKGGKLSGEVTGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFISFFMFK
RTYKRSQSFRDSVDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDNMTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=564711 KHW13_RS03565 WP_096134052.1 803525..804742(-) (pilC) [Pseudomonas syringae strain Susan762]
ATGGCCAGCAAGGCAGTTAAAGTCAGCGTCTACACGTGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGAGAAGT
GACAGGGCACAATCTCGCGCTCGTTAAGGCTCAGTTGCGCAAACAAGGCATCAATTTCACCAAGGTACGTAAAAAGCCCG
TTTCCATCTTTGGTAAAGGCAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCTCGCCAGATGGCAACCATGATGAAG
GCAGGTGTGCCACTGCTGCAGTCATTTGACATCATTTCCGAGGGCGCAGAAAACCCGAACATGCGTGCGCTCGTCGGTTC
ACTAAAGCAGGAGGTTTCAGCAGGGAACAGCTTTGCGACCGCTCTGCGAAAGAAACCGGAATACTTCGACGACCTGTTCT
GCAACCTGGTCGATGCCGGCGAGCAAGCGGGTGCACTAGAGAGCCTGTTGGATCGCGTGGCGAGCTACAAGGAAAAAACG
GAAAGGCTAAAGGCCAAAATCAAGAAGGCGATGACGTATCCTACCGCGGTATTAATTGTCGCCTTTATCGTGTCAGGTAT
TTTGTTGATTAAGGTCGTGCCGCAGTTCCAGTCTGTCTTCGCTGGGTTTAACGCGGAATTACCAGCGTTCACATTGATGG
TCATCGCGCTTTCGGAGGTCGTTCAGCAATGGTGGCTGGCGATAGTCGTATTGTTTTTTATCAGCTTTTTCATGTTTAAG
CGTACATACAAACGGTCTCAAAGTTTTCGTGACAGCGTCGACCGCTTTCTTCTGAAAGTGCCCATTATCGGACCGCTGAT
TTTCAAGTCGTCCGTCGCACGTTATGCACGCACGCTTGCGACAACCTTTGCGGCAGGCGTGCCGTTGGTTGAGGCACTAG
ATTCGGTTGCCGGAGCCACCGGGAACGTCGTATTCAGAAACGCGGTCAATCAGGTCAAACAGGATGTCTCTACTGGCATG
CAACTGAATTTCTCGATGCGGTCAACAGGCGTATTTCCAAGCCTGGCAATACAAATGACAGCAATCGGCGAAGAGTCCGG
TGCGCTTGACGCCATGCTCGATAAAGTCGCTACCTACTATGAAGAGGAAGTCGACAACATGGTCGACAACATGACCAGTC
TGATGGAGCCGATGATCATGGCGGTGCTGGGAGTTATTGTCGGCGGCCTTGTGATTGCCATGTACCTGCCCATCTTCAAA
CTTGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.168

99.753

0.58
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilG Neisseria gonorrhoeae MS11

41.687

99.506

0.415
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Thermus thermophilus HB27

38.06

99.259

0.378