Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHW13_RS03570 Genome accession   NZ_CP074411
Coordinates   804745..806439 (-) Length   564 a.a.
NCBI ID   WP_096134053.1    Uniprot ID   -
Organism   Pseudomonas syringae strain Susan762     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 799745..811439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW13_RS25645 - 799829..800065 (-) 237 WP_327397062.1 DUF3094 family protein -
  KHW13_RS03535 (KHW13_03535) - 799950..800483 (-) 534 WP_213568008.1 MOSC domain-containing protein -
  KHW13_RS03540 (KHW13_03540) - 800480..801109 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  KHW13_RS03545 (KHW13_03545) - 801237..801698 (+) 462 WP_213568009.1 hypothetical protein -
  KHW13_RS03550 (KHW13_03550) yacG 801825..802034 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  KHW13_RS03555 (KHW13_03555) coaE 802031..802654 (-) 624 WP_004415092.1 dephospho-CoA kinase -
  KHW13_RS03560 (KHW13_03560) pilD 802651..803523 (-) 873 WP_096134051.1 A24 family peptidase Machinery gene
  KHW13_RS03565 (KHW13_03565) pilC 803525..804742 (-) 1218 WP_096134052.1 type II secretion system F family protein Machinery gene
  KHW13_RS03570 (KHW13_03570) pilB 804745..806439 (-) 1695 WP_096134053.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW13_RS03575 (KHW13_03575) - 806665..807060 (+) 396 WP_096134054.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KHW13_RS03580 (KHW13_03580) - 807248..808528 (+) 1281 WP_347256466.1 O-antigen ligase family protein -
  KHW13_RS03585 (KHW13_03585) - 808640..809926 (+) 1287 WP_096134055.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  KHW13_RS03590 (KHW13_03590) - 809976..810521 (-) 546 WP_004415097.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62583.88 Da        Isoelectric Point: 6.2561

>NTDB_id=564712 KHW13_RS03570 WP_096134053.1 804745..806439(-) (pilB) [Pseudomonas syringae strain Susan762]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCNHCKKELDIPRETLIREGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=564712 KHW13_RS03570 WP_096134053.1 804745..806439(-) (pilB) [Pseudomonas syringae strain Susan762]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAATTGGTCCTGGCCGAGCTACTTACTGAAACGGCTGCTCAGCAGGC
TTACCAACAGGCGCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTTG
CAGAAATGGCCTCGGACCAGTTTGGCGTGCCCTTCATGGACCTTGCCAGTCTCGACAAGGAAAGCCAACCCAAAGGGTTG
GTCAGTGAAAAGCTGATTCGCCAGCATCATGCACTTCCGTTGTGGCGGCGTGGTAACAAGCTGTTTGTAGGCATCTCGGA
CCCGACCAACCATCAGGCGGTCACAGACATACAGTTCAGCACCGGCCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATAGCCATAGACCGTTTTTTTGACACTGAAAGCGGCCTTGGCGAGTTGGCAGATATCGACCTTGGCCTTGAT
ATCGAGCCTGCGAGTGACAAGGAAACATCACTGGCAACTCAGAGTGATGCCGATGATGCCCCGGTCGTGCGCTTCGTCAA
CAAGATGCTGATGGACGCCATACGGCTCGGTTCGTCCGACTTGCACTTTGAACCCTACGAAAAAATCTTTCGCGTGCGCC
TGCGTACCGACGGCATTTTGCATGAAGTGGCTAGGCCACCAATCCATCTGGCCAACCGGATTGCCGCACGGCTCAAGGTT
ATGGCCAGTCTCGATATCTCTGAGCGGCGTAAACCTCAAGACGGCAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCGAT
CGACTTCCGCATGAACACACTGCCAACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACCAGCGCGCAGA
TGGGCATTGATGCACTGGGCTACGAACCGGAGCAAAAGGCGCTTTATATGGAGGCCTTGAAACAGCCCCAAGGCATGATT
CTTGTGACCGGCCCGACTGGGTCAGGCAAAACCGTTTCGCTATACACCGGCCTGAACATTCTCAACACCGTAGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAGGGTATCAATCAGGTCAATGTCAATCCGCGACAGGGTCTGG
ACTTTTCCCAGGCATTGCGTGCCTTTCTGCGTCAAGATCCCGACGTGATCATGGTCGGTGAGATCCGGGACCTGGAAACC
GCCGAAATAGCCATCAAAGCCTCGCAAACCGGTCACATGGTGTTATCGACCCTGCACACCAACAGCGCTGCGGAAACGCT
GACTCGCCTGCACCATATGGGCGTCGCAGCGTTCAACATCGCTACAGCAATCAATCTCATCATTGCCCAGCGGCTGGCAC
GCAAGCTATGTAATCATTGCAAGAAAGAACTCGACATACCTCGTGAAACACTCATACGAGAGGGCTTCCCGGAAGAAAAA
ATAGGTACGTTCAAAATCTACGGCCCTGTTGGCTGCGAGCATTGCAATGGCGGCTATAGGGGCAGGGTCGGGATCTACGA
AGTGGTCAAGAAAACACCTGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATCTCCAGGCAGATGCGAA
AGGACGGCTTCAATGACTTGAGGACCTCCGGCCTGTCGAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

100

0.587
  pilB Acinetobacter baylyi ADP1

57.473

99.645

0.573
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.802

99.468

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.361

98.227

0.495
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilF Neisseria gonorrhoeae MS11

47.865

99.645

0.477
  pilF Thermus thermophilus HB27

39.399

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369