Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFZ68_RS01950 Genome accession   NZ_CP074084
Coordinates   392659..394350 (-) Length   563 a.a.
NCBI ID   WP_284647937.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd1411     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 387659..399350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFZ68_RS01920 (KFZ68_01920) mutT 388212..388619 (-) 408 WP_069530749.1 8-oxo-dGTP diphosphatase MutT -
  KFZ68_RS01925 (KFZ68_01925) yacG 388679..388888 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  KFZ68_RS01930 (KFZ68_01930) zapD 388897..389634 (-) 738 WP_005297594.1 cell division protein ZapD -
  KFZ68_RS01935 (KFZ68_01935) coaE 389702..390304 (-) 603 WP_068945536.1 dephospho-CoA kinase -
  KFZ68_RS01940 (KFZ68_01940) pilD 390306..391184 (-) 879 WP_284647935.1 A24 family peptidase Machinery gene
  KFZ68_RS01945 (KFZ68_01945) pilC 391367..392593 (-) 1227 WP_284647936.1 type II secretion system F family protein Machinery gene
  KFZ68_RS01950 (KFZ68_01950) pilB 392659..394350 (-) 1692 WP_284647937.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFZ68_RS01955 (KFZ68_01955) pilA 394350..394745 (-) 396 WP_182079581.1 pilin Machinery gene
  KFZ68_RS01960 (KFZ68_01960) ampD 395131..395709 (+) 579 WP_086367527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KFZ68_RS01965 (KFZ68_01965) pdhR 396069..396842 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62511.14 Da        Isoelectric Point: 5.4754

>NTDB_id=563089 KFZ68_RS01950 WP_284647937.1 392659..394350(-) (pilB) [Photobacterium damselae strain Pdd1411]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTVVSVHDYDFEACCKAVYQRD
LVLRHRALPLCQSANTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDEERDQATDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=563089 KFZ68_RS01950 WP_284647937.1 392659..394350(-) (pilB) [Photobacterium damselae strain Pdd1411]
ATGCACACTACTCTCGCCTCGATTCTACACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGTATATTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATTTTTGGTCTCACCGTCGTTTCGGTTCATGACTACGACTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
TTGGTATTACGTCATCGCGCACTACCGTTATGCCAAAGCGCCAATACGCTATTTTTAGGGGTCAGCGATCCATGCAACAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGACGCAATATTGAACCGCTACTACTAGAAAACAAACAGCTCGAATCAG
CTATCCGTCGTATTTATGGTCGTGAGTTAGGCGAAACAACCAATATGGCCAGTCGAGATGTCAGCGACGATGATCTGAAT
AACTTAGTCGATATTGGTGATGAAGAGCGAGATCAAGCGACAGATCTAGGTCAAGAAAACGAACCTGTTACCCGCTATAT
CAACCAAGTACTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATTCATTTTGAACCTTATGAGACCAGTTATCGGATCC
GATTTCGTTGTGATGGGATCTTGCACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTCTCAACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTTCGGGTTTCTACCCTACCTACTTTATGGGGGGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATGACAGGGCCGACGGGCAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATCTCTACCGCTGAAGATCCGGTAGAGATCAACTTACCTGGCATTAATCAGGTACACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTGGGGGAAATCCGCGATCTGGAA
ACTGCCTCTATTGCCGTTAAAGCAGCGCAAACTGGACACTTAGTGCTATCGACCTTGCACACCAATTCTGCTGCCGAAAC
CATCACCCGCTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGCCGACTTGCTCAAGCTCTGGTTGATGGTGCGACTACCTTAGAGCTGGAAAATATCGCAGTAGAGG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.634

100

0.616
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Vibrio campbellii strain DS40M4

60.391

100

0.604
  pilB Legionella pneumophila strain ERS1305867

50

96.27

0.481
  pilB Acinetobacter baylyi ADP1

48.723

97.336

0.474
  pilB Acinetobacter baumannii D1279779

50.958

92.718

0.472
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.499

92.185

0.364
  pilB Haemophilus influenzae 86-028NP

41.856

86.146

0.361
  pilB Haemophilus influenzae Rd KW20

41.856

86.146

0.361