Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSYCIT7_RS03635 Genome accession   NZ_CP073636
Coordinates   828061..829278 (-) Length   405 a.a.
NCBI ID   WP_003371261.1    Uniprot ID   -
Organism   Pseudomonas syringae Cit 7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823061..834278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYCIT7_RS03605 (PSYCIT7_003605) - 823279..823746 (+) 468 WP_003371252.1 hypothetical protein -
  PSYCIT7_RS03610 (PSYCIT7_003610) - 824186..825658 (+) 1473 WP_003430733.1 tetratricopeptide repeat protein -
  PSYCIT7_RS03615 (PSYCIT7_003615) - 825764..826222 (+) 459 WP_003371256.1 hypothetical protein -
  PSYCIT7_RS03620 (PSYCIT7_003620) yacG 826361..826570 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYCIT7_RS03625 (PSYCIT7_003625) coaE 826567..827190 (-) 624 WP_003350248.1 dephospho-CoA kinase -
  PSYCIT7_RS03630 (PSYCIT7_003630) pilD 827187..828059 (-) 873 WP_003430731.1 A24 family peptidase Machinery gene
  PSYCIT7_RS03635 (PSYCIT7_003635) pilC 828061..829278 (-) 1218 WP_003371261.1 type II secretion system F family protein Machinery gene
  PSYCIT7_RS03640 (PSYCIT7_003640) pilB 829281..830975 (-) 1695 WP_003430729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYCIT7_RS03645 (PSYCIT7_003645) - 831202..831636 (+) 435 WP_003371267.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSYCIT7_RS03650 (PSYCIT7_003650) - 831887..832099 (-) 213 Protein_721 winged helix-turn-helix transcriptional regulator -
  PSYCIT7_RS03655 (PSYCIT7_003655) - 832147..832368 (+) 222 WP_003371270.1 glutathione binding-like protein -
  PSYCIT7_RS03660 (PSYCIT7_003660) - 832713..833300 (+) 588 WP_172831880.1 DUF433 domain-containing protein -
  PSYCIT7_RS03665 (PSYCIT7_003665) - 833348..833893 (-) 546 WP_003371273.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43990.99 Da        Isoelectric Point: 10.2269

>NTDB_id=559640 PSYCIT7_RS03635 WP_003371261.1 828061..829278(-) (pilC) [Pseudomonas syringae Cit 7]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQSVFAGFGADLPAFTLMVIGLSKIVQEWWLLIVGLLFSGFFLFK
RAYKKSQKFRDGLDRLLLKAPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=559640 PSYCIT7_RS03635 WP_003371261.1 828061..829278(-) (pilC) [Pseudomonas syringae Cit 7]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTGGACAAGAAAGGCACTAAAACCAGTGGAGAACT
GAGCGGCCATAACCTGGCACTTGTAAAAGCACAACTGCGCAAGCAAGGCATCAATCCGACCAAAGTGCGCAAGAAGTCCG
CTTCGATCTTCGGTAAGGGCAAGAAAATAAAGCCACTGGATATAGCTTTTTTTTCTCGGCAGATGGCCACAATGATGAAA
GCCGGCGTTCCATTGCTGCAGTCGTTTGACATCATCAGCGAGGGTGCTGAAAACCCGAACATGCGTACGCTCGTGGGTTC
ACTCAAGCAGGAGGTGTCGGCCGGCAACAGCTTTGCGACGGCATTGCGACAGAAACCGGAATATTTCGATGACTTGTTTT
GCAACTTGGTAGATGCCGGTGAGCAAGCAGGGGCACTTGAGAGTCTGCTGGACCGAGTGGCAAGCTATAAGGAAAAGACG
GAAAAGCTCAAGGCTAAAATAAAAAAAGCAATGACGTACCCAATCGCCGTGTTGATCGTAGCGATTATTGTATCAGGTAT
TTTGTTGATCAAAGTGGTACCGCAGTTTCAATCCGTGTTTGCTGGTTTTGGTGCGGACTTACCTGCGTTCACTCTAATGG
TTATTGGTCTTTCAAAAATTGTTCAGGAGTGGTGGCTGTTAATCGTTGGCCTATTGTTTTCAGGATTTTTTTTATTTAAA
CGGGCTTACAAAAAATCTCAAAAATTTCGCGACGGACTTGATCGTCTTTTGCTAAAGGCACCACTCATTGGGCCTCTTAT
TTTCAAATCTTCGGTAGCTCGCTATGCGCGCACACTTGCTACGACTTTTGCCGCTGGTGTACCGCTTGTGGAGGCGTTGG
ACTCGGTTGCTGGAGCCACAGGCAATGTAGTGTTCAAAAATGCAGTCAATAAGGTCAAGCAAGACGTTTCCACAGGTATG
CAATTGAATTTCTCGATGCGATCTACGGGAGTATTCCCAAGCCTTGCGATCCAGATGACAGCCATTGGCGAAGAATCCGG
CGCCCTGGACACCATGCTCGACAAGGTAGCTACTTATTACGAGGATGAAGTCGATAACATGGTCGACAACCTGACCAGCC
TGATGGAACCTATGATCATGGCCTTTTTGGGAGTGATCGTCGGCGGCCTTGTTATTGCCATGTATCTCCCCATCTTCAAA
CTCGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

60.341

100

0.612
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

52.217

100

0.523
  pilC Vibrio campbellii strain DS40M4

43.5

98.765

0.43
  pilG Neisseria gonorrhoeae MS11

42.714

98.272

0.42
  pilG Neisseria meningitidis 44/76-A

42.714

98.272

0.42
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Thermus thermophilus HB27

38.404

99.012

0.38