Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSYCIT7_RS03640 Genome accession   NZ_CP073636
Coordinates   829281..830975 (-) Length   564 a.a.
NCBI ID   WP_003430729.1    Uniprot ID   A0A8T8LZH3
Organism   Pseudomonas syringae Cit 7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824281..835975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYCIT7_RS03615 (PSYCIT7_003615) - 825764..826222 (+) 459 WP_003371256.1 hypothetical protein -
  PSYCIT7_RS03620 (PSYCIT7_003620) yacG 826361..826570 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYCIT7_RS03625 (PSYCIT7_003625) coaE 826567..827190 (-) 624 WP_003350248.1 dephospho-CoA kinase -
  PSYCIT7_RS03630 (PSYCIT7_003630) pilD 827187..828059 (-) 873 WP_003430731.1 A24 family peptidase Machinery gene
  PSYCIT7_RS03635 (PSYCIT7_003635) pilC 828061..829278 (-) 1218 WP_003371261.1 type II secretion system F family protein Machinery gene
  PSYCIT7_RS03640 (PSYCIT7_003640) pilB 829281..830975 (-) 1695 WP_003430729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYCIT7_RS03645 (PSYCIT7_003645) - 831202..831636 (+) 435 WP_003371267.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSYCIT7_RS03650 (PSYCIT7_003650) - 831887..832099 (-) 213 Protein_721 winged helix-turn-helix transcriptional regulator -
  PSYCIT7_RS03655 (PSYCIT7_003655) - 832147..832368 (+) 222 WP_003371270.1 glutathione binding-like protein -
  PSYCIT7_RS03660 (PSYCIT7_003660) - 832713..833300 (+) 588 WP_172831880.1 DUF433 domain-containing protein -
  PSYCIT7_RS03665 (PSYCIT7_003665) - 833348..833893 (-) 546 WP_003371273.1 DUF1523 family protein -
  PSYCIT7_RS03670 (PSYCIT7_003670) - 834013..835011 (+) 999 WP_003371275.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62594.85 Da        Isoelectric Point: 6.3336

>NTDB_id=559641 PSYCIT7_RS03640 WP_003430729.1 829281..830975(-) (pilB) [Pseudomonas syringae Cit 7]
MTDAVLTGLAKQLVLAELLTESVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDTGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEEK
IGTFKIYGPVGCEHCNSGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFHDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=559641 PSYCIT7_RS03640 WP_003430729.1 829281..830975(-) (pilB) [Pseudomonas syringae Cit 7]
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTACTTACTGAATCTGTTGCGCAACAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAACTGGTCAAAAGTCTGACACTGG
CAGAGATGGCTTCCGACCAGTTTGGCGTGCCATTCATGGACCTCGCCAGTCTGGACAAAGAAAGCCAGCCTAAAGGCCTA
GTGAGTGAAAAGCTGGTTCGACAGCATCATGCCTTGCCGCTGTGGCGACGTGGGAACAAGCTGTTCATTGGCATATCGGA
CCCGACCAATCATCAGGCAGTCACCGATATTCAGTTCAGCACCGGCCTGAATACCGAAGCGATTCTGGTAGAAGATGACA
AGCTGACCACTGCAATAGATCGCTTTTTTGACAGTGATACCGGTCTAGGTAATCTGGAAGATGTCGATCTGGGCTTGGAT
ATCGAACCGGCCGATGGCAAGGAAACCTCTCTAGCTACACAGAATGATGCTGACGATGCTCCAGTAGTGCGATTTGTCAA
CAAAATGCTTATGGATGCCATCCGATTAGGATCATCCGACTTGCACTTCGAGCCCTACGAAAAGATCTTCCGTGTACGCT
TGCGTACCGATGGCATCCTGCACGAGGTCGCCAGACCGCCGATCCATCTGGCAAACCGTATTGCTGCGCGCCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCTAT
CGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAAAAGATTGTGATGCGGATCCTAGACCCGACCAGTGCGCAGA
TGGGTATTGATGCTCTGGGCTACGAGCCAGAACAAAAGGCACTGTATCTGGAAGCGCTGAAACAGCCGCAGGGCATGATT
CTCGTAACTGGTCCCACTGGGTCCGGCAAGACTGTTTCGCTGTATACAGGCCTGAATATTCTCAATACCGTAGACATCAA
CATTTCTACAGCCGAAGACCCGGTGGAGATTAACCTGGAAGGTATCAACCAAGTCAACGTCAATCCGCGTCAGGGGCTGG
ACTTTTCTCAGGCATTGCGCGCGTTCCTGCGTCAAGATCCCGACGTAATTATGGTCGGCGAGATTCGCGACTTGGAAACT
GCCGAAATCGCCATCAAGGCGTCACAGACCGGCCACATGGTGCTGTCAACCCTGCACACCAACAGTGCGGCAGAAACCCT
GACTCGCCTGCATCATATGGGTGTCGCAGCATTCAACATTGCTACAGCAATCAACCTGATTATTGCGCAGCGCCTTGCGC
GCAAGTTGTGCAGCCACTGCAAGAAAGAACTCGATATTCCTCGCGAGACACTGATTCGAGAAGGTTTTCCTGAAGAGAAG
ATTGGCACTTTCAAGATTTATGGCCCAGTGGGCTGTGAGCATTGCAATAGTGGCTATAGAGGCAGGGTCGGTATTTACGA
AGTCGTCAAGAAAACAGCGGAACTGGAGCGCATAATCATGGAAGAAGGCAACTCATTAGAGATTTCCAGGCAGATGCGTA
AGGACGGCTTCCACGACTTGCGAACCTCGGGCCTTTCGAAAGCTATGCAGGGCATCACCAGCCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.319

100

0.576
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.398

100

0.555
  pilB Vibrio cholerae strain A1552

50.534

99.645

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.357

99.291

0.5
  pilB Vibrio campbellii strain DS40M4

49.293

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

38.693

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362