Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KCG51_RS07790 Genome accession   NZ_CP073119
Coordinates   1632659..1633789 (+) Length   376 a.a.
NCBI ID   WP_250580379.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1626053..1638829 1632659..1633789 within 0


Gene organization within MGE regions


Location: 1626053..1638829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG51_RS07765 (KCG51_07775) - 1626053..1626202 (-) 150 WP_254342364.1 hypothetical protein -
  KCG51_RS07770 - 1626199..1628469 (-) 2271 WP_254342365.1 hypothetical protein -
  KCG51_RS07775 (KCG51_07785) lepB 1628808..1629827 (-) 1020 WP_250580372.1 signal peptidase I -
  KCG51_RS07780 (KCG51_07790) lepA 1629827..1631620 (-) 1794 WP_250580374.1 translation elongation factor 4 -
  KCG51_RS07785 (KCG51_07795) - 1631753..1632454 (-) 702 WP_250580376.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  KCG51_RS07790 (KCG51_07800) pilU 1632659..1633789 (+) 1131 WP_250580379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCG51_RS07795 (KCG51_07805) - 1633863..1635269 (-) 1407 WP_250580380.1 dihydrolipoyl dehydrogenase -
  KCG51_RS07800 (KCG51_07810) - 1635523..1636260 (-) 738 WP_250580381.1 redoxin family protein -
  KCG51_RS07805 (KCG51_07815) - 1636449..1637234 (-) 786 WP_250580382.1 1-acylglycerol-3-phosphate O-acyltransferase -
  KCG51_RS07810 (KCG51_07820) mutM 1637299..1638126 (-) 828 WP_250581319.1 DNA-formamidopyrimidine glycosylase -
  KCG51_RS07815 (KCG51_07825) - 1638170..1638829 (-) 660 WP_250580383.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42365.45 Da        Isoelectric Point: 5.8993

>NTDB_id=558172 KCG51_RS07790 WP_250580379.1 1632659..1633789(+) (pilU) [Neisseria subflava strain HP0069]
MFDENTPGAKEELFAWLRHMNKYKGSDLFITTNFPPAMKVDGKITRLSNEPLSAEKCMEIAFSIMSSKQIEEFSSTNECN
FAISLPDTSRFRVNAMVQRGATALVFRSITSNIPKFETLNLPPILKDIALRKRGLIIFVGGTGSGKSTSLASLIDYRNEN
SFDHIITIEDPIEFIHQHKNCIITQREIGVDTENWPIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHA
NSTNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPRESGRGRVAAVEVLLNSPIIADLIQKGEVHNIKEMMKKST
GMGMITFDQALYDLYENGDISYQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=558172 KCG51_RS07790 WP_250580379.1 1632659..1633789(+) (pilU) [Neisseria subflava strain HP0069]
ATGTTTGACGAAAATACTCCGGGCGCAAAAGAAGAATTGTTCGCTTGGCTACGCCATATGAACAAATACAAAGGCTCCGA
CCTTTTCATTACCACCAATTTCCCGCCAGCCATGAAAGTGGACGGCAAAATCACGCGACTAAGCAATGAGCCGCTAAGTG
CTGAAAAATGTATGGAAATCGCTTTTTCGATTATGTCTTCCAAACAAATCGAAGAATTTTCTTCCACCAACGAATGTAAC
TTCGCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTCAATGCAATGGTTCAACGCGGTGCAACTGCATTGGTGTTTCG
CTCCATTACCAGCAATATCCCGAAATTTGAAACGCTCAATCTGCCCCCTATTCTGAAAGACATTGCCCTCAGAAAACGCG
GCCTGATTATTTTTGTCGGCGGTACAGGTTCGGGCAAGTCCACTTCCCTCGCCTCCCTTATCGACTATCGAAACGAAAAC
AGCTTCGACCATATTATTACCATCGAAGACCCGATTGAGTTTATCCACCAACACAAAAACTGCATCATCACCCAACGTGA
AATCGGTGTAGATACAGAAAATTGGCCGATTGCGCTGAAAAACACCCTGCGCCAAGCGCCGGATGTTATTTTGATTGGCG
AAATTCGCGACCGTGAAACCATGGACTACGCTATTTCCTTCGCGGAAACCGGCCATTTGTGTATGGCGACACTGCATGCC
AACAGTACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTACCCAGTTGCTGACCGACTTGTC
GCTCAACCTGCAAGCCTTTATTTCACAACGTCTGATTCCCCGTGAAAGCGGCCGGGGTCGTGTGGCGGCGGTCGAAGTTT
TGCTCAATTCGCCGATTATTGCCGACTTGATTCAAAAAGGCGAAGTCCACAACATCAAAGAAATGATGAAAAAATCAACT
GGCATGGGCATGATCACTTTTGACCAAGCCTTGTATGATTTGTATGAAAACGGCGATATCAGCTATCAAGACGCCATTAA
AAATGCCGACTCCGCCCATGACTTGCGCTTAGACATTCAATTACGAAGCCGTCGTGCGCAAAATGCCGGTCCTGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.227

93.617

0.564
  pilU Acinetobacter baylyi ADP1

58.309

91.223

0.532
  pilU Vibrio cholerae strain A1552

52.925

95.479

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.243

88.564

0.383
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Legionella pneumophila strain Lp02

41.975

86.17

0.362
  pilT Legionella pneumophila strain ERS1305867

41.975

86.17

0.362
  pilT Acinetobacter baylyi ADP1

41.463

87.234

0.362