Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KCG56_RS05390 Genome accession   NZ_CP073116
Coordinates   1123934..1125064 (+) Length   376 a.a.
NCBI ID   WP_036490451.1    Uniprot ID   A0A9X9R050
Organism   Neisseria subflava strain TT0073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1118934..1130064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG56_RS05370 (KCG56_05415) - 1119152..1120030 (+) 879 WP_254322145.1 DMT family transporter -
  KCG56_RS05375 (KCG56_05420) lepB 1120085..1121104 (-) 1020 WP_036490457.1 signal peptidase I -
  KCG56_RS05380 (KCG56_05425) lepA 1121104..1122897 (-) 1794 WP_002226499.1 translation elongation factor 4 -
  KCG56_RS05385 (KCG56_05430) - 1123030..1123731 (-) 702 WP_254322146.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  KCG56_RS05390 (KCG56_05435) pilU 1123934..1125064 (+) 1131 WP_036490451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCG56_RS05395 (KCG56_05440) - 1125144..1126550 (-) 1407 WP_254322147.1 dihydrolipoyl dehydrogenase -
  KCG56_RS05400 (KCG56_05445) - 1126802..1127539 (-) 738 WP_188209826.1 redoxin family protein -
  KCG56_RS05405 (KCG56_05450) - 1127728..1128513 (-) 786 WP_254322148.1 1-acylglycerol-3-phosphate O-acyltransferase -
  KCG56_RS05410 (KCG56_05455) mutM 1128578..1129405 (-) 828 WP_188209828.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42451.60 Da        Isoelectric Point: 5.9015

>NTDB_id=558078 KCG56_RS05390 WP_036490451.1 1123934..1125064(+) (pilU) [Neisseria subflava strain TT0073]
MFDENTPGAKEELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKIMRLTDEPLSAEKCMEIAFSIMSSKQIEEFSTTNECN
FAISLPDTSRFRVNAMVQRGATALVFRSITSNIPKFETLNLPPILKDIALRKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFDHIITIEDPIEFIHQHKNCIITQREIGVDTENWPIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHA
NSTNQALDRIINFFPEERRNQLLTDLSLNLQAFISQRLIPREGGRGRVAAVEVLLNSPIIADLIRKGEVHNIKEMMKKST
GMGMITFDQALYDLYENGDISYQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=558078 KCG56_RS05390 WP_036490451.1 1123934..1125064(+) (pilU) [Neisseria subflava strain TT0073]
ATGTTTGACGAAAATACTCCGGGCGCAAAAGAAGAATTGTTCACTTGGCTACGCCACATGAACAAATACAAAGGCTCCGA
CCTTTTCATTACCACCAATTTCCCGCCGGCCATGAAAGTGGACGGCAAAATCATGCGGCTGACCGATGAGCCGTTAAGCG
CTGAAAAATGTATGGAAATCGCTTTTTCGATTATGTCTTCCAAACAAATCGAAGAATTTTCTACCACCAACGAATGCAAC
TTCGCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTCAATGCCATGGTTCAACGCGGCGCAACTGCTTTGGTGTTCCG
CTCCATTACCAGCAATATCCCTAAATTTGAAACGCTCAATCTACCGCCGATTCTGAAAGACATCGCCCTCAGAAAACGCG
GTCTGGTTATTTTTGTCGGTGGTACAGGTTCGGGCAAGTCCACTTCCCTCGCCTCCCTTATCGACTATCGAAATGAAAAC
AGCTTCGACCACATTATCACCATCGAAGATCCGATTGAATTTATCCACCAACATAAAAACTGCATTATCACCCAACGCGA
AATCGGCGTAGATACCGAAAATTGGCCGATTGCGCTGAAAAATACCCTGCGCCAAGCGCCGGATGTTATCCTGATTGGCG
AAATCCGCGACCGCGAGACCATGGACTACGCCATTTCCTTCGCGGAAACCGGCCATTTGTGTATGGCGACACTGCACGCC
AACAGTACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGTAACCAATTGCTAACCGATTTATC
GCTCAACCTGCAAGCCTTTATTTCACAACGCCTGATTCCCCGCGAAGGCGGACGGGGACGCGTAGCGGCTGTCGAGGTTT
TGCTCAATTCGCCCATTATTGCCGACTTGATTCGAAAAGGCGAAGTCCACAACATCAAAGAAATGATGAAAAAATCAACC
GGCATGGGCATGATTACTTTTGACCAAGCCTTGTATGATTTGTATGAAAACGGCGATATCAGCTATCAAGACGCCATTAA
AAATGCCGACTCTGCCCATGATTTGCGCTTAGACATTCAATTACGAAGCCGTCGCGCACAAAATGCCGGTCCGGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.08

93.617

0.572
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

53.482

95.479

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.844

88.564

0.388
  pilT Acinetobacter baumannii strain A118

43.519

86.17

0.375
  pilT Acinetobacter baumannii D1279779

43.519

86.17

0.375
  pilT Acinetobacter nosocomialis M2

43.519

86.17

0.375
  pilT Acinetobacter baylyi ADP1

42.378

87.234

0.37
  pilT Legionella pneumophila strain Lp02

42.593

86.17

0.367
  pilT Legionella pneumophila strain ERS1305867

42.593

86.17

0.367