Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   THIMO_RS04985 Genome accession   NC_019940
Coordinates   1130700..1131953 (-) Length   417 a.a.
NCBI ID   WP_015279999.1    Uniprot ID   L0GX20
Organism   Thioflavicoccus mobilis 8321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1125700..1136953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIMO_RS04965 (Thimo_1045) ubiA 1127390..1128295 (+) 906 WP_015279995.1 4-hydroxybenzoate octaprenyltransferase -
  THIMO_RS04970 (Thimo_1046) - 1128532..1128804 (-) 273 WP_015279996.1 hypothetical protein -
  THIMO_RS04975 (Thimo_1047) coaE 1128976..1129590 (-) 615 WP_015279997.1 dephospho-CoA kinase -
  THIMO_RS04980 (Thimo_1048) pilD 1129587..1130465 (-) 879 WP_015279998.1 A24 family peptidase Machinery gene
  THIMO_RS04985 (Thimo_1049) pilC 1130700..1131953 (-) 1254 WP_015279999.1 type II secretion system F family protein Machinery gene
  THIMO_RS04990 (Thimo_1050) pilB 1131959..1133674 (-) 1716 WP_015280000.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THIMO_RS04995 (Thimo_1051) - 1134321..1135154 (-) 834 WP_015280001.1 NYN domain-containing protein -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 45138.30 Da        Isoelectric Point: 9.9413

>NTDB_id=55433 THIMO_RS04985 WP_015279999.1 1130700..1131953(-) (pilC) [Thioflavicoccus mobilis 8321]
MAQVSLNKAAAKPKPVEAAAVFAWEGTDRRGKKLKGELRAPDMTAVRAELRRQGVSPTKVRKKPKPLFGGGKRKIKSADL
AVFTRQLATMMSAGVPLVQAFDIVGRGHENPAMQDLILAIKADIESGTGMAVALRKYPRYFDDLVCNLVAAGEQAGVLDT
LLDKIATYKEKTESIKGKIKKALFYPAAVIAVAVIVTTIILLFVIPQFKELFTSFGADLPAFTLLVIAISDFVHEWWWGI
GIGLVIAGFALKEAFQRSPKLREMLDRLSLKIPVIGQILNKAAIARFARTLSTMFSAGVPLVEALDSVSGATGNIVYANA
VQRMREDVATGQALQLTMRQQDLFPHMVIQMTAIGEESGALDEMLGKVADFFEEQVDNAVDALSSLLEPLIMVVIGSLVG
GLVIAMYLPIFKLAAVV

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=55433 THIMO_RS04985 WP_015279999.1 1130700..1131953(-) (pilC) [Thioflavicoccus mobilis 8321]
GTGGCTCAGGTATCGTTGAACAAAGCGGCCGCAAAGCCGAAGCCGGTCGAAGCGGCCGCCGTCTTTGCTTGGGAAGGCAC
CGACCGACGCGGCAAGAAACTCAAGGGCGAGTTGCGCGCCCCGGACATGACGGCGGTGCGCGCCGAGCTGCGCCGCCAAG
GCGTGAGCCCAACGAAGGTCCGCAAGAAGCCCAAGCCGCTCTTCGGGGGCGGCAAGCGCAAGATCAAGAGCGCCGACCTG
GCCGTCTTCACCCGGCAGCTCGCGACCATGATGTCGGCCGGCGTGCCCTTGGTCCAGGCGTTCGACATCGTCGGGCGCGG
CCATGAAAATCCAGCGATGCAGGATCTGATCCTCGCGATCAAGGCCGATATCGAAAGCGGCACCGGCATGGCCGTGGCCC
TGCGCAAATATCCCCGCTATTTCGACGATCTGGTCTGCAATCTGGTCGCCGCCGGCGAGCAGGCCGGCGTCTTGGATACG
CTGCTCGACAAGATCGCCACGTACAAGGAAAAGACCGAATCGATCAAGGGCAAAATCAAGAAGGCCCTATTCTATCCGGC
GGCCGTCATCGCGGTGGCCGTGATCGTGACGACGATCATCCTGCTGTTCGTCATCCCCCAGTTCAAGGAGCTCTTCACGA
GCTTCGGTGCCGACCTGCCGGCCTTCACGCTACTGGTGATCGCCATCTCCGACTTCGTGCACGAGTGGTGGTGGGGGATC
GGTATCGGACTCGTCATCGCCGGCTTTGCCCTGAAAGAAGCCTTCCAGCGATCACCCAAACTCCGCGAGATGCTCGACCG
CCTTTCGCTCAAGATCCCGGTGATCGGCCAAATACTGAACAAGGCCGCCATCGCCCGCTTCGCCAGGACTCTCTCGACCA
TGTTCTCCGCCGGCGTCCCCCTGGTCGAGGCGCTGGACTCGGTCTCCGGGGCGACGGGCAACATCGTCTACGCCAATGCC
GTGCAAAGGATGCGCGAGGATGTCGCCACGGGTCAGGCGTTGCAGTTGACGATGCGCCAGCAGGATCTCTTTCCCCACAT
GGTCATCCAGATGACAGCGATCGGCGAGGAATCAGGTGCACTGGACGAGATGCTCGGCAAGGTCGCCGATTTCTTCGAAG
AGCAGGTCGACAATGCGGTCGACGCCTTGAGCAGCCTCCTCGAGCCGCTGATCATGGTCGTCATCGGCAGCCTGGTCGGC
GGCCTGGTCATCGCGATGTACCTGCCCATCTTCAAGCTCGCCGCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(287-409)

Type II secretion system (T2SS), protein F (T2SSF)

(83-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GX20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.794

95.444

0.561
  pilC Acinetobacter baylyi ADP1

56.757

97.602

0.554
  pilC Legionella pneumophila strain ERS1305867

57.538

95.444

0.549
  pilC Acinetobacter baumannii D1279779

55.062

97.122

0.535
  pilG Neisseria gonorrhoeae MS11

45.614

95.683

0.436
  pilG Neisseria meningitidis 44/76-A

45.088

95.204

0.429
  pilC Vibrio campbellii strain DS40M4

42.929

94.964

0.408
  pilC Vibrio cholerae strain A1552

41.667

94.964

0.396
  pilC Thermus thermophilus HB27

36.779

99.76

0.367

Multiple sequence alignment